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ABSTRACT

TheIllinoisJuniorAcademyofScience
Thisform/papermaynotbetakenwithoutIJASauthorization.

CATEGORY

EnvironmentalScience

STATEREGION#

SCHOOL

NilesNorthHighSchool

IJASSCHOOL#

6038

CITY/ZIP

Skokie,IL60076

SCHOOLPHONE#

8476262256

SPONSOR

ChristineCamel

MARKONE:

EXPERIMENTALINVESTIGATION

DESIGNINVESTIGATION

NAMEOFSCIENTIST*

ZacharyLuna

GRADE

12

NAMEOFSCIENTIST

GRADE

NAMEOFSCIENTIST

GRADE

NAMEOFSCIENTIST

GRADE

*Ifthisprojectisawardedamonetaryprize,thecheckwillbewritteninthisscientist'sname,anditwillbe
his/herresponsibilitytodistributetheprizemoneyequallyamongallparticipatingscientists.

PROJECTTITLE

TheEffectofDifferentSolutionsofNitrogenonMicrobeInducedOil
DegradationinLakeMichigan


Purpose:Thepurposeofthislaboratoryexperimentistolearnabouttheconditionsthatwouldcauseoil
eatingbacterialocaltoLakeMichigantomultiply.Thegreatertheabundanceoftheoileatingbacteriathe
moreoilthebacteriawillbeabletoingest.Sincethesemicrobesareverysensitivetotheenvironmentthey
livein,testswouldbeusedtoseeifconditionslikewarmtemperaturesandincreasednitrogencancause
theseoileatingbacteriatomultiply.Testswillbeperformedonoillacedsoilsamplesderivedfromthe
MidwestnearLakeMichigan.
Procedure:ThisprocedureinvolvedcalculatingtheabsorbancelevelswithaSpectrophotometerof
contaminatedsoilsamplesascomparedtothesamplesafteroildegradingmicrobeswereintroducedwith
outsidevariables.Thesevariableswereappliedeitherbybeingmixedintothesamplesorsimplysprinkledon
top.ThevariablesintroducedincludeStrontiumNitrate,UreaandAmmoniumHydroxide.Theoilcontents
inpercentagebasedoffoftheabsorbanceforeachsampleafter7dayswasthencomparedtotheoriginaloil
contentspercentage.

Conclusion:
Overall,thedatadidsupportthehypothesis.AmmoniumHydroxidewasseenasthemosteffective
agentforaidingthemicrobesoilconsumption.Thissolutionwasthemosteffectivewhenitwasappliedbybeing
mixedintothegravelorsimplyplacedonthetop.TheamountofAmmoniumHydroxideusedwasalsocorrelated
totheamountorUreaandStrontiumnitratebypriceondosage.Eachdoseaddedtothesamplesfromeachsolution
costed.0078cents.ThismeansthatalthoughAmmoniumHydroxideisthemostexpensiveproductbeingabout
717$perton,itisstillthemoreeffectivesolutionforthemicrobesefficiencyevenifitisusedinlesseramounts.


1)LimitAbstractto3paragraphs(about200wordsorless).a)Purposewhatyousetouttoinvestigateb)
Procedurehowyoudid
itc)Conclusionbasedonyourresults.Labeleachparagraph.
2)Mustbetyped,singlespacedonthefrontofthisform.Donotwriteonthebackofthisform.
3)ThreecopiesofyourcompletepaperarerequiredattheStateScienceProjectExposition.
FourcopiesofyourcompletepaperarerequiredfortheStatePaperSessionCompetition.

Thisformmustbeused.Thisformmustbedisplayedonthefrontoftheexhibitorsdisplayboard.Itmay
bereducedtohalfasheetofpaper8.5inches(vertical)X5.5inches(horizontal).

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