-Add 10-20ul of sample to same amount of 2X loading buffer (1:1), mix well, and
include Protein Marker.
2X Loading buffer:
100 mM Tris-HCL (pH6.8)
200 mM DTT
4% SDS
0.2% Bromophenol blue
20% glycerol
(DTT should be added just before the buffer is used, from 1M stock)
-Boiling for 5-10 min
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