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Describe a method to find the rate of respiration in insect.

(5 marks)

Set up the apparatus as shown, make sure that the apparatus in airtight.
Sodium hydroxide in added into both test tubes to remove carbon dioxide
produced by the respiring insect and to have similar initial pressures in both
Place the insect in one test tube and glass beads of equal mass in the other
test tube.
Dip the test tubes in a water bath to keep temperature constant. As
temperature affects enzyme activity by maintaining a constant temperature
rate of respiration would remain constant throughout.
Measure the time taken for the fluid in the manometer to move a fixed
Rate of respiration = 1/ time taken
Take repeat readings by adjusting the screw clip and letting air in.