MAKALAH METODE LABORATORIUM

FERTILITAS
Metode Indirect Enzym-Linked Immunosorbent Assay
dan Aplikasinya Pada Penelitian Kesehatan Reproduksi

Oleh:

ANINDYA HAPSARI (011514653001)

Pembimbing:
Dr. Widjiati, M.Si., Drh

PROGRAM PASCA SARJANA ILMU KESEHATAN REPRODUKSI

UNIVERSITAS AIRLANGGA
2016

KATA PENGANTAR
Puji syukur kehadirat Allah SWT karena atas rakhmat dan petunjuk-Nya
semata, tugas pembuatan Makalah Metode Laboratorium Fertilitas dengan judul
Metode Indirect Enzym-Linked Immunosorbent Assay dan Aplikasinya Pada
Penelitian Kesehatan Reproduksi ini dapat penulis selesaikan. Makalah ini
penulis susun sebagai tugas mata kuliah Metode Laboratorium Fertilitas.
Pada penulisan makalah ini, penulis ingin menyampaikan ucapan terima
kasih kepada, Dr. Widjiati, MSi., Drh. selaku dosen pengampu Mata Kuliah

Metode Laboratorium Fertilitas atas bimbingan dan petunjuk beliau dalam

penyelesaian makalah ini.
Penulis menyadari bahwa makalah ini masih jauh dari kata sempurna
sehingga kritik dan saran yang membangun penulis harapkan dari semua pihak.

Surabaya, April 2016

Penulis

DAFTAR ISI
Halaman
SAMPUL
i

HALAMAN

DEPAN......................................................................................
KATA

ii

PENGANTAR........................................................................................
..............
DAFTAR

iii

ISI.........................................................................................................
............
DAFTAR

iv

LAMPIRAN...........................................................................................
........
BAB

PENDAHULUAN...................................................................................
..............
1.1

Latar

Belakang...............................................................................................
..............
1.2

Rumusan

Masalah.................................................................................................
.......
1.3

Tujuan....................................................................................................
.......................
1.3.1

Tujuan

Umum....................................................................................................
........
1.3.2

Tujuan

Khusus...................................................................................................
........
1.4

Manfaat.................................................................................................
........................
1.4.1

Manfaat

Teoritis...................................................................................................
.....
1.4.2

Manfaat

Praktis...................................................................................................
......
BAB

TINJAUAN

PUSTAKA.......................................................................................
2.1
Metode

Immunoassay........................................................................................
...........
2.2

Definisi

ELISA.....................................................................................................
........
2.3

Sejarah

Penggunaan

Metode

ELISA............................................................................
2.4
Prinsip
Dasar

Metode

ELISA......................................................................................
2.5
Jenis

Metode

ELISA....................................................................................................
2.6 Alat dan Bahan yang Digunakan dalam Metode Indirect

ELISA...............................
2.7

Tahapan

Metode

Indirect

2
1
4

ELISA.................................................................................
2.8
Interpretasi
Hasil

Indirect

ELISA................................................................................
2.9
Aplikasi
Metode

Indirect

7
1

ELISA.................................................................................
2.10
Kelebihan
dan
Kekurangan
Metode

Indirect

8
2

0
2

PEMBAHASAN.....................................................................................

.............
BAB

PENUTUP.............................................................................................

...............
4.1

ELISA...............................................
BAB

Kesimpulan........................................................................................

...........................
DAFTAR

PUSTAKA..............................................................................................

.........
LAMPIRAN...........................................................................................

...........................

9
DAFTAR LAMPIRAN

Jurnal 1.

Inhibin B Menurunkan Konsentrasi

Follicle

Halaman
39

Stimulating Hormone (FSH) pada Tikus Putih

(Rattus

norvegicus):

Kontrasepsi

Upaya

Hormon

Pengembangan
Pria

Berbasis

Peptida....................................................................
.....................
Jurnal 2. Endoglin (cd105) and S100A13 as Markers of
Active
Jurnal

Angiogenesis

44

in

Endometriosis...................................................
3. Usefulness of BP180 NC16a Enzyme-Linked

61

Immunosorbent Assay in the Serodiagnosis of

Pemphigoid
Between

Gestationis

Pemphigoid

Urticarial

and

in

Gestationis

Papuler

and

Differentiating
and
Plaques

Pruritic
of

Pregnancy...............................
Jurnal 4. Use of Recombinant Antigens for Early Postnatal
Diagnosis

of

67

Congenital

Toxoplasmosis.........................................................
.
Jurnal 5. Use of an Immunoglobulin G Avidity Assay Based
on Recombinant Antigens for Diagnosis of Primary
Toxoplasma

gondii

Infection

Pregnancy..............................................

during

76