"The following reagents will be necessary for the preparation of

normal human urine (Kark, et al. 1964):

Albumin powder (egg or bovine)

Creatinine

Distilled water

Potassium chloride

Sodium chloride

Sodium phosphate (monobasic)

Urea

A class of 30 students, working in groups of two, would require a

class total of at least 1 liter of artificial urine for specific
gravity and dipstick testing. The following instruction are for the
preparation of approximately 2 liters of normal urine; half can be
stored or used for abnormal urine studies.

To 1.5 liters of distilled water add 36.4 g of urea and mix until all
the crystals are dissolved. Then add 15.0 g of sodium chloride, 9.0 g
of potassium chloride and 9.6 g of sodium phosphate; mix until the
solution is clear. Check the pH with indicator paper or a pH meter to
ensure the pH is within the 5 to 7 pH range for normal urine; if the
solution is out of this pH range the pH may be lowered with 1N
hydrochloric acid or raised with 1N sodium hydroxide.

Next, place a urine hydrometer into the solution and dilute with water
until the solution is within the specific gravity range of 1.015 to
1.025. This solution will serve as the storage stock solution of
?normal urine solution? and may be kept refrigerated for several weeks
or frozen in plastic containers for months. Before use, the stock
solution should be warmed to room temperature. Then, to ensure a
similarity to human urine, 4.0 g of creatinine and 100 mg of albumin
may be slowly mixed into the 2 liters of the so-called normal urine
solution. "