ABSTRACT
This research work determined the bioactive constituents of some medicinal plants and their
effectiveness in the area of pharmacology or pharmaceutical. The phytochemical screening carried on
the leaves extract of A. indica, H. rosasinensis, I. cylindrical revealed the presence of some active
ingredients such as Alkaloids, Tannins, Saponins, and Phenols also glycosides, steroids, terpenoids,
flavonoids and phlobatanins are present in this extract. The result demonstrated that the presence of
phytochemical components in the leaves extract. It was observed that Alkanoids has the following
values, 0.52g in A. Indica, 0.51g in H. Rosasinensis, 0.42g in I. cylindrical, while Tannins also has the
following value 9.00g in A. indica, 8.40g in H. Rosasinensis, 9.20g in I. Cylindrical. Saponins has the
value of 1.99 in A. indica, 1.80 in H. rosasinensis, 1.30 in I. cylindrica. Flavonoids have the value of
0.62 in A. indica, 0.38 in H. rosasinensis, 0.32 in I cylindrica. Phenol has the values of 0.024 in A.
Indica, 0.600 in H. rosasinensis, .050 in I. cylindrica.
INTRODUCTION
From the inception of the existence of earth, plant has been of great importance to the animal kingdom. It
contributes either as a source of food or shelter. In the primitive ages, plants were also used for clothing
therefore provides the basic needs of humans and animals as well. Out of these plants medicinal plants are
common and are source of much attention in Africa. (Akenova, et al., 1996).
Uniformity of the quality of drug is also of great importance as far as the therapeutically active constituent of
raw material is concern.
This makes it possible for pharmacist to prescribe Numerical values through which commodities are assessed
and are used to ensure uniformity of standards.(Amen, 1996)
Medical plant (Akinmoladun, et al., 2007) is defined as one, which contains substance that can be used for
therapeutic purposes and its precursor for the synthesis of useful drugs. They contain nutrients that can heal the
body (Frease and Evans, 1985).
Material like these plants that has cellular structure is referred to as organized drug in pharmacy and those with
non-cellular structure as unorganized or a cellular drug (Akinpelu, et al., 2006).
Medicinal plants are termed as crude drugs of natural or biological origin by pharmacists to describe whole plant
or plant parts having medicinal properties (Okwu et al., 2006).
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K. E. Ayeni and Yahaya S,A: Continental J. Pharmaceutical Sciences 4: 47 - 50, 2010
The mixture is covered and allowed to stand for 4 hours. The mixture is then filtered and the extract is
allowed to become concentrated in a water bath until it reaches ¼ of the original volume. Concentrated
NH4OH is added until the precipitation is complete. The whole solution is allowed to settle and the
precipitate is collected and washed with dilute NH4OH and the filtered. The residue is alkaloid which is
then dried and weighed.
b) Tannins: Quantity of tannins is determined by using the spectrophotometer method. 0.5g of plant
sample is weighed into 50ml plastic bottle 50ml of distilled is added and stirred for 1h. the sample is
filtered into a 50ml volumentric flask and made up to mark 5ml of the filtered sample is then pipetted
out into test-tube and mixed with 2ml of 0.1m FeCl3 in 0.1m HCl and 0.008m K4Fe (CN)6 3H2O. The
absorbance is measured with a spectrophotometer at 395nm wavelength within 10min.
c) Saponins: The sample were ground and 20g of each plant sample is put into a conical flask and
100ml of 20% C2H5OH is added to the plant sample. The sample is heated over a hot water bath for 4
hours with continuous stirring at about 550C. The mixture is then filtered and the residue re-extracted
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K. E. Ayeni and Yahaya S,A: Continental J. Pharmaceutical Sciences 4: 47 - 50, 2010
d) with another 200ml of 20% C2H50H. Then the combined extracts are reduced to 40ml over a water
bath at about 900C.
The concentrated is then transferred into a 250mlseparation funnel and 20ml of (CH3CH2)2O is added to the
extract and shaken vigorously. The aqueous is recovered while the (CH3CH2)20 layer is discarded and the
purification process is repeated 60ml of N-C4H9OH is added and combined n-C4H9OH extracts is washed twice
with 10ml of 5% NaCl. The remaining solution is then heated in a water bath and after evaporation: the
samples are dried in the oven to a constant weight.
e) Flavonoids: 10g of plant sample is repeatedly extracted with 100ml of 80% aqueous methanol at
room temperature. The whole solution is then filtered through filter paper and the filtrate is later on
transferred into a water bath and solution is evaporated into dryness. The sample is then weighed
until a constant weight.
DISCUSSION
For the qualitative analysis results, below is the discussion. The research work that was carried out on the three
medicinal plants shows that phytochemical constituents are present in which it was summarized in Table 1.
It shows that tannins, saponins, flavonoids, terpenoid and alkaloid, phenol were present in all the three plants.
But phlobatannins were found to be absent in the three plants. Cardiac glycosides were present in A. indica and
I. cylindrica and found to be absent in H. rosasinensis.
A. indica and H. rosasinensis have the highest yield of alkaloid which is 0.5g in which I. Cylindrica has the
lower yield of alkaloid which is 0.4g. But A. Indica and I. cylindrica showed from the result to have highest
yield of Tannins which is 9.00g. Moreover A. indica, H. rosasinensis, and T. cylindrica have been founded to
have similar yielded of saponins which are 1.99, 1.80 and 1.30 percentages respectively.
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K. E. Ayeni and Yahaya S,A: Continental J. Pharmaceutical Sciences 4: 47 - 50, 2010
A. indica has the highest yield of flavonoids which is 0.62% followed by H. rosasinensis which is 0.38% and I.
cylindrica is found to have the least amount of flavonoid which is 0.32%.
Finally H. rosasnensis has the highest yield of phenols quantity and followed by A. indica and I. cylindrica
which are in similar or the same range.
CONCLUSION
This research work has revealed further potentials of these three plants in the area of pharmacology as potential
source of useful drugs. T his study therefore has provided some biochemical basis for ethno pharmacological
uses of these plants in the treatment and prevention of various diseases and disorders.
RECOMMENDATION
Since the results of the phytochemical screening have shown that the extract of the three samples contain
alkaloids, tannin, saponin, phlobatannin, terpenoid, flavonoid, cardiac glycoside and phenol.
Therefore I recommend that these extracts should be used in the production of drugs in the area of
pharmaceutical or pharmacology for the treatment of intestinal track, diarrhea, headache, skin disease and it
should be used as a source of pesticide and insecticide, also herbicide in the agricultural area so as to improve
the yield of crops and reduce pests, weeds and other organism which are competing with man.
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and antioxidant activity of extract from the leaves of Ocimum gratissimum. Sciences Research Essay 2: 163
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Okwu, D. E. and Josiah, C. (2006), Evaluation of the chemical composition of two Nigerian Medicinal plants
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Akenova, M.E. and Attackrah, A.N. (1986) Control of spear grass (imperata cylindrical {L} Bearuv in an alley
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Corresponding Author
K. E. Ayeni
Science Technology Department Federal Polytechnic, P.M.B 420, Offa
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