Rune A. Hartvig†, Manuel A.

Programa de Lic. en Edu. Básica con énfasis en Ciencias Naturales y Edu. Ambiental

Interfacial Complexes
between a Protein and
Lipophilic Ions at an
Oil−Water Interface
Rune A. Hartvig†, Manuel A. Mendez, Marco van de Weert, Lene
Jorgensen, Jesper Østergaard, Hubert H. Girault and Henrik Jensen.
Department of Pharmaceutics and Analytical Chemistry, Faculty of
Pharmaceutical Sciences, University of Copenhagen,

Yamit Castañeda
Lic. en Ciencias Naturales.
Seminario de Química.
Universidad del Tolima.
2010

Seminario de Química- Facultad de Ciencias-Universidad del Tolima Colombia

Keywords: complexation degree, detection lipophilic ions BESI-MS

 Programa de Lic. en Edu. Básica con énfasis en Ciencias Naturales y Edu. Ambiental

ABSTRACT

The interaction between an intact protein and two lipophilic

ions at an oil−water interface has been investigated using
cyclic voltammetry, impedance based techniques and a
newly developed method in which the biphasic oil−water
system is analyzed by biphasic electrospray ionization
mass spectrometry (BESI-MS), using a dual-channel
electrospray emitter. It is found that the protein forms
interfacial complexes with the lipophilic ions and that it
specifically requires the presence of the oil−water interface
to be formed under the experimental conditions.

Seminario de Química- Facultad de Ciencias-Universidad del Tolima Colombia

Programa de Lic. en Edu. Básica con énfasis en Ciencias Naturales y Edu. Ambiental

Furthermore, impedance based techniques and BESI-MS

with a common ion to polarize the interface indicated
that the Galvani potential difference across the oil−water
interface significantly influences the interfacial
complexation degree. The ability to investigate
protein−ligand complexes formed at polarized
liquid−liquid interfaces is thus a new analytical method
for assessing potential dependent interfacial
complexation using a structure elucidating detection
principle

Seminario de Química- Facultad de Ciencias-Universidad del Tolima Colombia

Programa de Lic. en Edu. Básica con énfasis en Ciencias Naturales y Edu. Ambiental

Seminario de Química- Facultad de Ciencias-Universidad del Tolima Colombia

 Programa de Lic. en Edu. Básica con énfasis en Ciencias Naturales y Edu. Ambiental

BIBLIOGRAFIA

1.Formation and study of single metal ion–phospholipid

complexes in biphasic electrospray ionization mass
spectrometry. Michel Prudent, Manuel A. Méndez, Daniel F. Jana,
Clémence Corminboeuf and Hubert H. Girault..Metallomics. 2010
2. Imágenes por:
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Seminario de Química- Facultad de Ciencias-Universidad del Tolima Colombia