Alleleustrious pmTFP1-EB3

Catalog Number: Introduction All ustrious pmTFP1-EB3 is a mammalian expression vector that encodes a fusion of human EB3 N-terminal to mTFP1. EB3, a ~31kD protein, is also known as microtubule-associated protein, RP/EB family, member 3 (MAPRE3), and APC binding protein. This product is a great marker for cytoplasmic microtubules. The properties of All ustrious mTFP1 are superior to those of the commonly used Aequorea victoria variant ECFP and which can be easily detected in the mitotic spindle of mammalian cells using standard CFP filter sets. In addition, the fluorescent signal from mTFP1 is significantly brighter than that from EGFP using standard GFP filter sets. mTFP1 also makes an excellent fluorescence resonance energy transfer (FRET) donor to yellow or orange fluorescent proteins. EB3 encodes a protein of 282 amino acids with 54% identity of EB1, both proteins were found to associate with the cytoplasmic microtubule network. EB3 is preferentially expressed in brain tissue and appears together with APCL, a CNS-specific APC (adenomatous polyposis coli) tumor suppressor. APCL-EB3 interaction may be specific to the CNS, possibly involving stability and extension of microtubules of neuritogenesis.
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ABP-FP-TEB3

Source Engineered variant of Clavularia sp. fluorescent protein cFP484. Recommended Use To further optimize detection, the use of filter sets specific to the mTFP1 excitation and emission spectra is recommended. Features More than 3-fold brighter than ECFP More than twice as photostable as ECFP Single emission peak Single-exponential fluorescence lifetime ( = 3.2 ns) Can be co-imaged with red and yellow FPs Low sensitivity to acidic pH (fluorescence pKa=4.3) Mammalian expression vector ready to transfect your favorite cells True monomer that will not aggregate or cause non-specific interactions Reconstitution 5 g provided in lyophilized powder form. Reconstitute with 5 L of nuclease-free water for a final concentration of 1 g/L. Storage Store at -20C or at -80C for long-term preservation.

Human CMV Immediate-Early Promoter (CMV Promoter)........1-589 EB3.............................................652-1494 Linker......1495-1515 mTFP1.1516-2226 SV40 PolyA Signal.....................2378-2428 bla Promoter...............................2967-3071 Ampicillin Resistance Gene......3056-3916 pUC Origin..................................4065-4707 Upstream Sequencing Primer: Universal CMV Promoter Primer Downstream Sequencing Primer: SV40 Primer:GCTTT ATTTG TGAAA TTTGT GATGC TATTG C BLA SV40 Poly A mTFP1 AMPR pUC Ori PCMV

pmTFP1-EB3 4789bp

EB3

Linker

References: Ai HW, Hazelwood KL, Davidson MW, Campbell RE. Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nature Methods. 2008 5(5): 401-03. Ai HW, Henderson JN, Remington SJ, Campbell RE. Directed evolution of a monomeric, bright, and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging. Biochem J. 2006. Shaner NC, Steinbach PA, Tsien RY. A guide to choosing fluorescent proteins. Nat Methods. 2005 2(12):905-09. Nakagawa, H., Koyama, K., Murata, Y., Morito, M., Akiyama, T., Nakamura, Y. " EB3, a novel member of the EB1 family preferentially expressed in the central nervous system, binds to a CNS-specific APC homologue." Oncogene 2000 19(2) 210-6. Juwana JP, Henderikx P, Mischo A, et al.. "EB/RP gene family encodes tubulin binding proteins.". Int. J. Cancer 1999 81 (2): 27584

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmTFP1-EB3
Sequence
1 61 121 181 241 301 361 421 481 541 601 661 721 781 841 901 961 1021 1081 1141 1201 1261 1321 1381 1441 1501 1561 1621 1681 1741 1801 1861 1921 1981 2041 2101 2161 2221 2281 2341 2401 2461 2521 2581 2641 2701 2761 2821 2881 2941 3001 3061 3121 3181 3241 3301 3361 3421 3481 3541 3601 3661 3721 3781 3841 3901 3961 4021 4081 4141 4201 4261 4321 4381 TAGTTATTAA CGTTACATAA GACGTCAATA ATGGGTGGAG AAGTACGCCC CATGACCTTA CATGGTGATG ATTTCCAAGT GGACTTTCCA ACGGTGGGAG CCGGACTCAG AATGTGTACT GTCAACGACT TACTGCCAGT CAGGCCAAAC AAGATGGGTG AATTTTGAGT TACAACCCTC CAGATCTTCA ACAGGCCCAA CTCCGGAAGA GAACTCAACC GACTTCTACT AACAGCCCTG CCCCCTGAGG CCACCGGTCG GACATGAAGA GGCGAGGGCG GGAGCCCCCC GCCTTCACCA TACTCTTGGG ATCTCCATGG CCCAACGGCC TACGTGCGCG GGCCACCACC GACTATCACT GTGACCGTTT AAGTAAGCGG TGCTTTAAAA TGTTGTTAAC TTTCACAAAT TGTATCTTAA GCGTGACCGC TTCTCGCCAC TCCGATTTAG GTAGTGGGCC TTAATAGTGG TTGATTTATA AAAAATTTAA GGGAAATGTG GCTCATGAGA TATTCAACAT TGCTCACCCA GGGTTACATC ACGTTTTCCA TGACGCCGGG GTACTCACCA TGCTGCCATA ACCGAAGGAG TTGGGAACCG AGCAATGGCA GCAACAATTA CCTTCCGGCT TATCATTGCA GGGGAGTCAG GATTAAGCAT ACTTCATTTT AATCCCTTAA ATCTTCTTGA GCTACCAGCG TGGCTTCAGC CCACTTCAAG GGCTGCTGCC GGATAAGGCG TAGTAATCAA CTTACGGTAA ATGACGTATG TATTTACGGT CCTATTGACG TGGGACTTTC CGGTTTTGGC CTCCACCCCA AAATGTCGTA GTCTATATAA ATCTCGAGCT CCACATCTGT CCCTGCACCT TCATGGACAT TAGAGCATGA TTGACAAAAT TTATTCAGTG TGCTGGCGCG ACAAATCCAA AAAACATGCA ATCCTCCATC AACAGCTGGT TCAGCAAACT TTATCTCAGG ACGATGAGAT CCACCATGGT TCAAGCTGAA AGGGCAAGCC TGCCCTTCTC AGTACCCCGA AGCGCACCAT AGGAGGACTC CCGTGATGCA ACGGCGTGCT GCGTTGACTT TTGTGGACCA ACGAGAGCGC CCGCGACTCT AACCTCCCAC TTGTTTATTG AAAGCATTTT CGCGTCCTGT TACACTTGCC GTTCGCCGGC TGCTTTACGG ATCGCCCTGA ACTCTTGTTC AGGGATTTTG CGCGAATTTT CGCGGAACCC CAATAACCCT TTCCGTGTCG GAAACGCTGG GAACTGGATC ATGATGAGCA CAAGAGCAAC GTCACAGAAA ACCATGAGTG CTAACCGCTT GAGCTGAATG ACAACGTTGC ATAGACTGGA GGCTGGTTTA GCACTGGGGC GCAACTATGG TGGTAACTGT TAATTTAAAA CGTGAGTTTT GATCCTTTTT GTGGTTTGTT AGAGCGCAGA AACTCTGTAG AGTGGCGATA CAGCGGTCGG TTACGGGGTC ATGGCCCGCC TTCCCATAGT AAACTGCCCA TCAATGACGG CTACTTGGCA AGTACATCAA TTGACGTCAA ACAACTCCGC GCAGAGCTGG CAAGCTTCGA GACCAGTGAA CAACTATACC GCTCTTCCCC ATACATCCAC CATTCCTGTA GTTTAAGAAA GCAGGGCCAG GAAACTCATT GACCTCTGGC AGCCCGAAAT GGACTTGAAG TCGTGACATC CATCATTGGC TGAAGAGCAT GAGCAAGGGC GATGGAGGGC CTACGACGGC CTACGACATT CGACATCCCC GACCTTCGAG CTTCATCTAC GAAGAAGACC GAAGGGCGAC CAAGACCATC CCGCATCGAG CGTGGCCCGC AGATCATAAT ACCTCCCCCT CAGCTTATAA TTTCACTGCA AGCGGCGCAT AGCGCCCTAG TTTCCCCGTC CACCTCGACC TAGACGGTTT CAAACTGGAA CCGATTTCGG AACAAAATAT CTATTTGTTT GATAAATGCT CCCTTATTCC TGAAAGTAAA TCAACAGCGG CTTTTAAAGT TCGGTCGCCG AGCATCTTAC ATAACACTGC TTTTGCACAA AAGCCATACC GCAAACTATT TGGAGGCGGA TTGCTGATAA CAGATGGTAA ATGAACGAAA CAGACCAAGT GGATCTAGGT CGTTCCACTG TTCTGCGCGT TGCCGGATCA TACCAAATAC CACCGCCTAC AGTCGTGTCT GCTGAACGGG ATTAGTTCAT TGGCTGACCG AACGCCAATA CTTGGCAGTA TAAATGGCCC GTACATCTAC TGGGCGTGGA TGGGAGTTTG CCCATTGACG TTTAGTGAAC ATTCTGCAGT AATCTGAGTC AAGATAGAAC GGCTGTGTGC AACTTCAAGG GAGAAATTAG TTCTTTGACG GACGTAGCGC GGCACAGCAG CGGCTGAGCA GGCGGCCATG CTGACAGTGG GAGCTCATCT ATCCTCTATG CAACAAGAAG GAGGAGACCA AACGTGAATG ACCAACACCA CTGACCACCG AACTACTTCA GACAAGGGCA GAGATACACC ACCGGCTGGG GTCAAGCACA TACAGGGCCA ATCCTGAACC AACTCCACCG CAGCCATACC GAACCTGAAA TGGTTACAAA TTCTAGTTGT TAAGCGCGGC CGCCCGCTCC AAGCTCTAAA CCAAAAAACT TTCGCCCTTT CAACACTCAA CCTATTGGTT TAACGCTTAC ATTTTTCTAA TCAATAATAT CTTTTTTGCG AGATGCTGAA TAAGATCCTT TCTGCTATGT CATACACTAT GGATGGCATG GGCCAACTTA CATGGGGGAT AAACGACGAG AACTGGCGAA TAAAGTTGCA ATCTGGAGCC GCCCTCCCGT TAGACAGATC TTACTCATAT GAAGATCCTT AGCGTCAGAC AATCTGCTGC AGAGCTACCA TGTTCTTCTA ATACCTCGCT TACCGGGTTG GGGTTCGTGC AGCCCATATA CCCAACGACC GGGACTTTCC CATCAAGTGT GCCTGGCATT GTATTAGTCA TAGCGGTTTG TTTTGGCACC CAAATGGGCG CGTCAGATCC CGACTGCCAC GCCATGATAT AGCTTTGTTC ACTTGAGGAA TGCTGCAAGC TGAAAGGAAA CAAACTATGA CACCTCCTAA TTCCACAGAG ATGTGGCCCC AGACTGATGC ATGGGCTGGA GCCAGGAGCA CCACAGAGGA ACCAGGACGA CAATGGGCGT GCCACGCCTT TCAACCTGGA CGTTCGCCTA AGCAGTCCTT TCGTGAAGGT TCAAGGGCGA ACGCCTCCAC AGCTGCTGCT AGAAGGCGGT ACGACAAGGA ACGGCATGGA ACATTTGTAG CATAAAATGA TAAAGCAATA GGTTTGTCCA GGGTGTGGTG TTTCGCTTTC TCGGGGGCTC TGATTAGGGT GACGTTGGAG CCCTATCTCG AAAAAATGAG AATTTAGGTG ATACATTCAA TGAAAAAGGA GCATTTTGCC GATCAGTTGG GAGAGTTTTC GGCGCGGTAT TCTCAGAATG ACAGTAAGAG CTTCTGACAA CATGTAACTC CGTGACACCA CTACTTACTC GGACCACTTC GGTGAGCGTG ATCGTAGTTA GCTGAGATAG ATACTTTAGA TTTGATAATC CCCGTAGAAA TTGCAAACAA ACTCTTTTTC GTGTAGCCGT CTGCTAATCC GACTCAAGAC ACACAGCCCA TGGAGTTCCG CCCGCCCATT ATTGACGTCA ATCATATGCC ATGCCCAGTA TCGCTATTAC ACTCACGGGG AAAATCAACG GTAGGCGTGT GCTAGCGCTA CATGGCCGTC GCTTGCATGG AGGGGCAGCC AGTGAAGTTC AGCTTTCAAG ATTCCAAGAT TGGAAAGGAT CCCAGGTGAT GACGTCCCCC CCCCTGCATT CCAAATTCTT GAAGGAACGT TGAAAGTGAA AGGATTCGCA GTACCTGGAT AATCAAGCCC CGTGATCGAG GGTGAAGGAG CGGCAACAGG CCCCGAGGGC GAAGTCCGAC GAACTTCCCC CGAGAGGATG GGAGGGCGGC GAAGCTGCCC CTACAACAAG CGAGCTGTAC AGGTTTTACT ATGCAATTGT GCATCACAAA AACTCATCAA GTTACGCGCA TTCCCTTCCT CCTTTAGGGT GATGGTTCAC TCCACGTTCT GTCTATTCTT CTGATTTAAC GCACTTTTCG ATATGTATCC AGAGTATGAG TTCCTGTTTT GTGCACGAGT GCCCCGAAGA TATCCCGTAT ACTTGGTTGA AATTATGCAG CGATCGGAGG GCCTTGATCG CGATGCCTGT TAGCTTCCCG TGCGCTCGGC GGTCTCGCGG TCTACACGAC GTGCCTCACT TTGATTTAAA TCATGACCAA AGATCAAAGG AAAAACCACC CGAAGGTAAC AGTTAGGCCA TGTTACCAGT GATAGTTACC GCTTGGAGCG

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmTFP1-EB3
4441 4501 4561 4621 4681 4741 AACGACCTAC CGAAGGGAGA GAGGGAGCTT CTGACTTGAG CAGCAACGCG TCCTGCGTTA ACCGAACTGA AAGGCGGACA CCAGGGGGAA CGTCGATTTT GCCTTTTTAC TCCCCTGATT GATACCTACA GGTATCCGGT ACGCCTGGTA TGTGATGCTC GGTTCCTGGC CTGTGGATAA GCGTGAGCTA AAGCGGCAGG TCTTTATAGT GTCAGGGGGG CTTTTGCTGG CCGTATTACC TGAGAAAGCG GTCGGAACAG CCTGTCGGGT CGGAGCCTAT CCTTTTGCTC GCCATGCAT CCACGCTTCC GAGAGCGCAC TTCGCCACCT GGAAAAACGC ACATGTTCTT

Enzyme AatII Acc113I Acc16I AccB1I AccB7I AccBSI AccI AccIII AcsI AcyI AfeI AflIII AgeI AhdI Alw21I Alw44I AlwNI Ama87I AocI Aor51HI ApaLI ApoI AseI AsnI Asp700I AspEI AspHI AvaI AviII BalI BamHI BanI BanII BbiII BbsI Bbv12I Bbv16II BcgI BclI BcoI BglI BglII BlpI BpiI BpmI Bpu1102I Bpu14I BpuAI BsaAI BsaBI BsaHI BsaI BsaMI BsaOI BsaWI Bse118I Bse21I Bse8I BseAI BseRI BsgI Bsh1285I Bsh1365I BshNI BsiEI BsiHKAI BsiI BsiMI BsmI BsoBI Bsp119I Bsp13I Bsp1407I Bsp143II Bsp1720I Bsp19I BspCI BspEI BspHI BspLU11I BsrBI BsrBRI BsrDI

Cuts 6 1 1 4 3 2 1 1 7 8 1 2 1 1 6 3 1 2 1 1 3 7 2 2 1 1 6 2 1 1 1 4 4 8 2 6 2 1 1 2 6 2 1 2 4 1 1 2 3 1 8 2 3 5 6 5 1 1 1 2 1 5 1 4 5 6 2 1 3 2 1 1 1 6 1 4 1 1 2 1 2 1 3

Positions 125 178 261 447 1135 2012 3362 3620 465 1648 2659 3890 792 1275 1955 2559 3004 640 1202 629 949 988 1253 2398 2883 2894 122 175 258 444 1132 1961 2009 3303 596 2470 4731 1503 3843 620 821 1355 3175 3260 4421 817 3171 4417 4322 613 1792 1445 596 817 3171 4417 629 949 988 1253 2398 2883 2894 7 3668 7 3668 3243 3843 620 821 1355 3175 3260 4421 613 1792 3620 1601 1497 465 1648 2659 3890 620 1355 1686 2629 122 175 258 444 1132 1961 2009 3303 1482 1949 620 821 1355 3175 3260 4421 1482 1949 3327 1077 613 1792 90 212 283 652 2083 3725 609 1082 1174 1482 1949 1311 1671 2034 3758 1174 627 1482 1949 340 1983 2700 2246 122 175 258 444 1132 1961 2009 3303 1539 3776 1201 2334 2433 1508 2231 3325 3474 4397 600 1202 1503 3547 4378 4525 1169 1503 1951 2595 3758 1445 2246 1202 1536 1875 738 1508 2231 3325 3474 4397 2246 465 1648 2659 3890 1508 2231 3325 3474 4397 620 821 1355 3175 3260 4421 3179 4563 1202 1201 2334 2433 613 1792 627 1202 2216 598 1060 2180 2545 2553 4491 1174 360 650 1514 1865 3474 1202 3003 4011 4731 2559 3004 2246 1183 3607 3789

Sequence gacgt/c agt/act tgc/gca g/gyrcc ccannnn/ntgg gagcgg gt/mkac t/ccgga r/aatty gr/cgyc agc/gct a/crygt a/ccggt gacnnn/nngtc gwgcw/c g/tgcac cagnnn/ctg c/ycgrg cc/tnagg agc/gct g/tgcac r/aatty at/taat at/taat gaann/nnttc gacnnn/nngtc gwgcw/c c/ycgrg tgc/gca tgg/cca g/gatcc g/gyrcc grgcy/c gr/cgyc gaagac gwgcw/c gaagac cgannnnnntgc t/gatca c/ycgrg gccnnnn/nggc a/gatct gc/tnagc gaagac ctggag gc/tnagc tt/cgaa gaagac yac/gtr gatnn/nnatc gr/cgyc ggtctc gaatgc cgry/cg w/ccggw r/ccggy cc/tnagg gatnn/nnatc t/ccgga gaggag gtgcag cgry/cg gatnn/nnatc g/gyrcc cgry/cg gwgcw/c ctcgtg t/ccgga gaatgc c/ycgrg tt/cgaa t/ccgga t/gtaca rgcgc/y gc/tnagc c/catgg cgat/cg t/ccgga t/catga a/catgt gagcgg gatnn/nnatc gcaatg

BsrFI BsrGI BssAI BssSI BssT1I BstBI BstD102I BstDSI BstEII BstH2I BstI BstMCI BstPI BstSFI BstSNI BstX2I BstXI BstYI BstZI Bsu36I CciNI CelII Cfr10I CfrI Csp45I CvnI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1104I Eam1105I EarI Ecl136II EclHKI EclXI Eco105I Eco130I Eco24I Eco255I Eco31I Eco47III Eco52I Eco57I Eco64I Eco81I Eco88I Eco91I EcoICRI EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I ErhI Esp1396I FauNDI FbaI FriOI FspI GsuI HaeII Hin1I HincII HindII HindIII HpaI Hsp92I Kpn2I Ksp22I Ksp632I LspI MamI MfeI MflI MluI

5 1 5 2 4 1 2 5 1 6 1 5 1 7 1 11 1 11 1 1 1 1 5 7 1 1 4 1 2 2 5 7 1 3 1 3 2 1 1 1 4 4 1 2 1 1 5 4 1 2 1 2 1 1 1 1 4 1 4 3 1 1 4 1 4 6 8 5 5 1 1 8 1 1 3 1 1 1 11 1

1169 1503 1951 2595 3758 2216 1169 1503 1951 2595 3758 3179 4563 360 650 1514 1865 627 2559 3004 360 650 1514 1865 2180 2160 598 1060 2180 2545 2553 4491 1497 1508 2231 3325 3474 4397 2160 634 926 2070 2477 3597 4275 4466 340 585 609 1082 1497 2129 3196 3213 3981 3993 4079 4090 1665 585 609 1082 1497 2129 3196 3213 3981 3993 4079 4090 2228 1445 2228 1174 1169 1503 1951 2595 3758 653 1167 1233 1599 2040 2228 3450 627 1445 2286 3265 3957 3976 1739 2112 2703 2747 4629 360 650 1514 1865 2180 653 1167 1233 1599 2040 2228 3450 2228 807 1467 3054 3843 807 1467 3054 618 1353 3843 2228 340 360 650 1514 1865 620 1355 1686 2629 3362 1539 3776 596 2228 1334 1581 2004 3161 4209 465 1648 2659 3890 1445 613 1792 2160 618 1353 1070 1739 2160 629 360 650 1514 1865 4788 360 650 1514 1865 792 1275 1955 234 1077 620 1355 1686 2629 3620 1311 1671 2034 3758 598 1060 2180 2545 2553 4491 122 175 258 444 1132 1961 2009 3303 641 723 912 2055 2347 641 723 912 2055 2347 622 2347 122 175 258 444 1132 1961 2009 3303 1202 1077 807 1467 3054 627 2246 2334 585 609 1082 1497 2129 3196 3213 3981 3993 4079 4090 2470

r/ccggy t/gtaca r/ccggy ctcgtg c/cwwgg tt/cgaa gagcgg c/crygg g/gtnacc rgcgc/y g/gatcc cgry/cg g/gtnacc c/tryag tac/gta r/gatcy ccannnnn/ntgg r/gatcy c/ggccg cc/tnagg gc/ggccgc gc/tnagc r/ccggy y/ggccr tt/cgaa cc/tnagg ttt/aaa rg/gnccy cacnnn/gtg gacnnnn/nngtc c/crygg y/ggccr c/ggccg ctcttc gacnnn/nngtc ctcttc gag/ctc gacnnn/nngtc c/ggccg tac/gta c/cwwgg grgcy/c agt/act ggtctc agc/gct c/ggccg ctgaag g/gyrcc cc/tnagg c/ycgrg g/gtnacc gag/ctc cctnn/nnnagg rg/gnccy g/gtnacc g/aattc c/cwwgg atgca/t c/cwwgg ccannnn/ntgg ca/tatg t/gatca grgcy/c tgc/gca ctggag rgcgc/y gr/cgyc gty/rac gty/rac a/agctt gtt/aac gr/cgyc t/ccgga t/gatca ctcttc tt/cgaa gatnn/nnatc c/aattg r/gatcy a/cgcgt

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmTFP1-EB3
MluNI Mph1103I MroI MroNI MscI MslI Msp17I MspA1I MunI Mva1269I NaeI NcoI NdeI NgoAIV NgoMI NheI NotI NsiI NspBII NspI NspV PaeR7I PflMI PinAI Ple19I Ppu10I PshBI Psp124BI 1 1 1 2 1 9 8 4 1 3 2 4 1 2 2 1 1 1 4 3 1 1 3 1 1 1 2 2 1601 4788 1202 1169 2595 1601 365 676 796 1373 1516 1864 3072 3431 3590 122 175 258 444 1132 1961 2009 3303 1275 3207 4148 4393 2334 1201 2334 2433 1171 2597 360 650 1514 1865 234 1169 2595 1169 2595 591 2228 4788 1275 3207 4148 4393 801 1158 4735 627 613 792 1275 1955 1503 3474 4784 7 3668 620 1355 tgg/cca atgca/t t/ccgga g/ccggc tgg/cca caynn/nnrtg gr/cgyc cmg/ckg c/aattg gaatgc gcc/ggc c/catgg ca/tatg g/ccggc g/ccggc g/ctagc gc/ggccgc atgca/t cmg/ckg rcatg/y tt/cgaa c/tcgag ccannnn/ntgg a/ccggt cgat/cg a/tgcat at/taat gagct/c Psp1406I PspEI PstI PstNHI PvuI PvuII RcaI SacI SalI SapI ScaI SfcI Sfr274I SfuI SnaBI SspBI SspI SstI StyI Van91I VneI VspI XbaI XcmI XhoI XhoII XmaIII XmnI Zsp2I 2 1 1 1 1 1 2 2 1 2 1 7 1 1 1 1 2 2 4 3 3 2 1 1 1 11 1 1 1 3241 3614 aa/cgtt 2160 g/gtnacc 638 ctgca/g 591 g/ctagc 3474 cgat/cg 1275 cag/ctg 3003 4011 t/catga 620 1355 gagct/c 639 g/tcgac 807 1468 gctcttc 3362 agt/act 634 926 2070 2477 3597 4275 4466 c/tryag 613 c/tcgag 627 tt/cgaa 340 tac/gta 2216 t/gtaca 2908 3038 aat/att 620 1355 gagct/c 360 650 1514 1865 c/cwwgg 792 1275 1955 ccannnn/ntgg 817 3171 4417 g/tgcac 7 3668 at/taat 2238 t/ctaga 2202 ccannnnn/nnnntgg 613 c/tcgag 585 609 1082 1497 2129 3196 3213 r/gatcy 3981 3993 4079 4090 2228 c/ggccg 3243 gaann/nnttc 4788 atgca/t

The following enzymes do not cut: AatI, Acc65I, AclNI, AflII, ApaI, AscI, Asp718I, AspI, AtsI, AvrII, BanIII, BbeI, BbrPI, BbuI, BfrI, BlnI, Bsa29I, BscI, BseCI, BsePI, BsiWI, BsmBI, Bsp106I, Bsp120I, Bsp68I, BspDI, BspMI, BspTI, BspXI, BssHII, Bst1107I, Bst98I, Bsu15I, Cfr42I, Cfr9I, ClaI, CpoI, CspI, Eco147I, Eco32I, Eco72I, EcoRV, EheI, Esp3I, FseI, KasI, KpnI, KspI, MspCI, NarI, NruI, PacI, PaeI, Pfl23II, PmaCI, Pme55I, PmeI, PmlI, PpuMI, PshAI, Psp5II, PspAI, PspALI, PspLI, PspOMI, RsrII, SacII, SbfI, SexAI, SfiI, Sfr303I, SgfI, SgrAI, SmaI, SmiI, SpeI, SphI, SplI, SrfI, Sse8387I, SseBI, SstII, StuI, SunI, SwaI, Tth111I, Vha464I, XmaI

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Current All ustious Fluorescent Protein Family Members: The founding member is mTFP1. mTFPG3 is a green FP with 3 amino difference from mTFP1. It has a slightly red-shifted emission spectrum and is 1.5 fold brighter compared to EGFP. While being very bright, mTFPG3 can be photobleached within ~5 sec, about 30 times faster than EGFP, suitable for certain cell-based assays that require a bright FP with very short half-life. mTFP0.7 is a precursor during the evolution of mTFP1. It has photo-switchable properties like Dronpa that cycles between fluorescent and nonfluorescent states. It may be developed into components in PALM/SIM applications.
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Basic Vectors Three vectors are available: pNCS-mWasabi, pmWasabi-N and pmWasabi-C. Subcellular Marker Vectors Twenty six vectors are available. Vectors in Viral Vectors All plasmid format vectors in Allele's Phoenix Retroviral vector or HiTiter Lentiviral Vectors.

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Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010