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Designing a Lab

Caitlin Barrett

Research Question: Does the addition of one or more worms (Lumbricus terrestris) affect
the amount of CO2 produced within an enclosed bell jar?

Hypothesis: The addition of worms to the bell jar would increase the amount of CO2
produced due to the respiration of the worms. The worms would absorb the oxygen when it
inhales and send out carbon dioxide when it exhales would would increase the amount
present within the enclosed area. Due to the fact that it is in an enclosed area with no outer
source of oxygen, if left in the bell jar for an extended amount of time the worms will die as
the jar will end filled with carbon dioxide.

Variables:
Independent: the worm(s)
Dependent: CO2

Controlled: the amount of worms


the size of the jar
the amount of time the worm(s) are left for
the size of the worms
the type of worms

Apparatus:
10 worms (Lumbricus terrestris)
A bell jar
A CO2 detector attached to a computer
4 petrie dishes to place the worms in (one for each trial)
Stop watch

Method:
1.Place one worm in a petrie dish and place it inside the enclosed bell jar with the
CO2 detector inside as well.
2.Allow the worm to stay inside the bell jar for 30 minutes and allow the CO2
detector to collect the data for this time period
When the time is up, record the results on a data table and remove the worm from
the bell jar
3.Place 2 worms in the second petrie dish and place back under the bell jar for 30
minutes with the CO2 detector collecting data
4.Once again, when the time is complete, remove the petrie dish with the worms on
it and record the data found from this trial
5.Repeat steps 1 and 2 with three worms and four worms and record the data
6. When all 10 worms have been used compare the data and see the differences in
CO2 production based on the number of worms present in the bell jar.

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