Penalty Failure to turn in by due date will result in 5 point reduction in grade.
Directions Give brief, but complete answers to each of the following questions.
Place answers in spiral bound notebook. Skip a space between each answer.
1. a. What anticoagulant is used to collect samples for most routine hematology tests?
b. How does this anticoagulant work?
4. List the three hemoglobins found in normal adult blood and state how much of each is present.
8. Name three conditions that may interfere with the cyanmethemoglobin method for hemoglobin.
9. a. What are the dimensions of a Newbauer hemacytometer on one side of the counting chamber?
b. What is the depth of the Newbauer hemacytometer?
c. What is the depth factor used in calculations?
12. What is the generic formula for calculating a manual blood cell count using a Newbauer
hemacytometer?
13. a. What is the formula used to correct a WBC count for the presence of NRBC’s?
b. When must a WBC count be corrected for NRBCs?
14. For each of the following RBC indices, write the formula for calculation and the normal reference
range.
a. MCV
b. MCH
c. MCHC
16. a. What term is used to describe a variation in RBC size within a single patient sample?
b. What term is used to describe a variation in RBC shape within a single patient sample?
18. State how each of the following will affect a patient’s sed rate (increased, decreased):
a. presence of macrocytes
b. tilt tube
c. air bubble in tube
d. incubation temperature > RT
e. increased plasma acute phase reactants
20. On a normal peripheral smear, how many platelets should be seen per oil immersion field?
21. How do you estimate the number of platelets using a peripheral smear?
22. State how each of the following conditions will affect a patient’s platelet count (increase, decrease).
a. specimen not well mixed upon collection
b. Polycythemia vera
c. normal response to blood loss
d. myelofibrosis
e. post-splenectomy
f. DIC
25. What two stains are present in the Wright stain used for peripheral blood smears?
26. How do you estimate the number of WBCs using a peripheral smear?
27. Name the cell type seen on a peripheral smear described by each of the following:
a. nucleus contains several lobes connected by strands of chromatin, abundant cytoplasm with
granules
b. nucleus stains deep purple and may be round or slightly notched, small amount of “robin’s egg
blue” cytoplasm
c. nucleus is horse-shoe shape with delicate chromatin, blue-gray cytoplasm which has “ground
glass” appearance
d. nucleus is band or sausage shape, abundant cytoplasm with granules
e. bi-lobed nucleus, cytoplasm contains many acidophilic granules
29. What are the two main types (principle) of automated cell counters?
30. Using the Coulter principle, what five analytes are actually measured by the instrument?
33. List the three categories of cells displayed on a WBC histogram (from left to right).
34. How is cell sizing of the WBCs performed on automated counter? Be specific.
37. Give three reasons why the above curve would only show a smooth curve.
41. Name the two sites normally used for bone marrow aspiration in an adult.
42. List the stages of development of the red blood cell from most immature to the mature red blood cell.
Use both sets of names.
44. Retics
a. What stain is used to perform a retic stain?
b. A technologist counts 25 retics in 700 RBCs on a patient with a hematocrit of 28 %. What is the
patient’s corrected retic count?
46. Name the substance that normally attaches to the beta chains of hemoglobin A to prevent the binding
of oxygen in the tissues.
47. For each method of hemoglobin electrophoresis, give the pH of the buffer used and list the
hemoglobins in order of migration starting at the anode.
48. Kleihauer-Betke
a. What cells are resistant to denaturation by acid and stain orange using eosin?
b. Why is the K-B stain usually performed?
49. Give the scientific name for each abnormal RBC type.
a. Helmet cell
b. Burr cell
c. Teardrop cell
d. Cell fragment
e. Target cell
f. Target cell with target connected to cell membrane
g. Sickle cell
h. Mouth cell
i. Round cell with no central pallor
j. Cigar shaped cells
53. What term refers to a decrease in the number of all blood cell types?
55. Which anemia is associated with the following finding on the peripheral smear?
a. hypersegmented PMNs
b. basophilic stippling
c. target cells
d. Pappenheimer bodies
e. drepanocytes
f. Heinz bodies
56. What amino acid substitution in the globin chains is seen with each of the following. Be specific.
a. sickle cell anemia
b. Hemoglobin C disease
59. List the stages of development in the granulocytic series in the order of maturation.
61. List the stages of development in the monocytic series in the order of maturation.
62. List the stages of development in the lymphocytic series in the order of maturation.
66. For each of the following classification of acute leukemia, give the name.
a. M1
b. M2
c. M3
d. M4
e. M5
f. M6
g. M7
h. L1
i. L2
j. L3
73. Name the type of malignant disorder associated with the following:
a. hypogammaglobulinemia
b. Philadelphia chromosome
c. presence of Bence Jones proteins in urine
d. Mycosis fungoides
e. monoclonal increase in IgG
f. monoclonal increase in IgM
g. presence of Reed-Sternberg cells
74. State the chromosomal translocation that occurs with the Philadelphia chromosome.
75. State the normal value for the following Hematology tests.
a. RBC
b. WBC
c. Platelet
d. Hemoglobin
e. Hematocrit
f. RDW
g. Diff
h. Retic