BIOFILM
DETOXIFICATION


A
New
Approach
To
Trea7ng
Chronic

Gastrointes7nal
Infec7ons



The
Wellness
Center
for
Research
&
Educa7on,
Inc.


Theresa
Dale,
PhD,
CCN

Tradi7onal
Naturopath


1
BIOFILM


•  What
is
Biofilm?

•  How
are
they
formed?

•  Where
do
they
grow?

•  What
disease
do
they

cause?


2
 Quote


…
If
I
needed
to
remove
either
the
medical
or

dental
component
of
my
clinic,
I
would
keep

the
Dental
because
chronic
problems
will
not

resolve
without
Biological
Dental
care…..


“Dr.
Thomas
Rau,
Director
of
Paracelsus
Clinic,

Switzerland”.


3
 BioFilm
Overview

•  Figh7ng
chronic
infec7ons
is
a
problem
that
has

become
more
and
more
difficult
with
which
to
deal.


Scien7sts
worldwide
are
now
seeing
microorganisms
as

evolved,
complex
colonies
that
have
developed
unique

and
intricate
ways
to
survive.


•  We
all
are
supposed
to
have
normal,
healthy,
symbio7c

flora
growing
in
our
GI
tract
that
helps
us
fight
off

pathogens
and
allergens.
When
the
balance
of
good

bugs
and
bad
bugs
is
disrupted,
our
immune
system

cannot
func7on
op7mally
and
chronic
infec7ons
and

allergy
issues
may
ensue.



4
 Overview

•  Remember
that
70%
of
our
immune
system
resides
in

our
gut.

When
our
immune
system
is
weak,
pathogens

find
a
way
to
set
up
residence
in
our
body
and
GI
tract.


In
order
to
evade
the
immune
system
and
survive
in
a

hos7le,
toxic
environment,
these
bugs
produce
a
slimy

substance
called
biofilm.


•  
This
biofilm
surrounds
and
protects
the
bad
bugs
like

an
impenetrable
fortress
allowing
them
to
thrive
and

reproduce.

As
they
reproduce,
they
con7nue
to

produce
their
toxic
byproducts
that
may
have

detrimental
effects
on
the
health
of
the
host
without

the
host
even
realizing
the
bugs
are
there.



5
 Overview

•  Recently,
scien7sts
are
discovering
ways
to
penetrate

these
pathogenic
biofilms
and
expose
the
bad
bugs

underneath.

They
have
discovered
the
composi7on
of

most
biofilms
consists
of
sugar
molecules
or

polysaccharides,
minerals
(such
as
calcium,
iron
and

magnesium),
DNA
and
fibrin.



•  Some
studies
have
shown
that
by
using
enzymes
that

poten7ally
degrade
components
of
the
pathogenic

biofilm,
infec7ons
in
the
lungs,
sinuses
and
wounds
are

easier
to
clear.



6
 Overview

•  BioFilm
Detox
contains
a
combina7on
of
unique

enzymes
that
have
the
ability
to
dissolve
the

sugar
and
fibrin
components
of
most
pathogenic

biofilms.



•  Biofilm
Detox
can
be
taken
on
an
empty
stomach.


•  Clinicians
may
use
this
product
on
an
empty

stomach
followed
by
appropriate
BioTox
Nosode

products,
(homeopathic
pathogens)
that
target
a

specific
bug
or
bugs.


7
 Overview

•  Naturopathic
or
Holis7c
programs
targe7ng

the
GI
tract
include
quality
enzymes
(Digest

Pro),
probio7cs,
prebio7cs,
and
nutrients

(Op7mal
Mul7
Pro,
Nano
Ionic
Mul7,
Vitamin

D,
etc.)
that
help
to
rebuild
and
heal
the
gut

lining
and
allow
our
symbio7c
flora
to
become

restored.



8
 Tes7ng

•  C‐reac7ve
protein
as
a
marker
for

inflammatory
bowel
disease.


9
 What
is
Biofilm?

•  A
biofilm
is
a
accumula7on
of
microorganism’s
(bacteria,

fungi,
protozoa
with
associated
bacteriophages
&
other

viruses)
imbedded
in
a
polysacaride
matrix
which
adheres
to

solid
biologic
or
non‐biologic
surface.



•  Biofilm
is
a
collec7on
of
microbial
communi7es
enclosed
by

a
matrix
of
extracellular
polymeric
substance
(EPS)
and

separated
by
a
network
of
open
water
channels.


•  These
communi7es
adhere
to
manmade
and
natural

surfaces,
such
as
metals
and
teeth,
typically
at
a
liquid‐solid

interface
.
Their
architecture
is
an
op7mal
environment
for

cell‐cell
interac7ons,
including
the
intercellular
exchange
of

gene7c
material,
communica7on
signals,
and
metabolites,

which
enables
diffusion
of
necessary
nutrients
to
the
biofilm

community.



•  The
matrix
is
composed
of
a
nega7vely
charged

polysaccharide
substance,
held
together
with
posi7vely

charged
metal
ions
(calcium,
magnesium,
and
iron).


Staphylococcus aureus
biofilm •  The
matrix
in
which
microbes
in
a
biofilm
are
embedded

protects
them
from
UV
exposure,
metal
toxicity,
acid

exposure,
dehydra7on
salinity,
phagocytosis,
an7bio7cs,

an7microbial
agents
and
the
immune
system.


10
 Con7nued..

•  For
example,
dental
plaque
is
biofilm.


•  Biofilms
are
clinically
important,
accoun7ng
for
over
80
percent
of

microbial
infec7ons
in
the
body.



•  Other
examples
include:
infec7ons
of
the
oral
sod
7ssues,
teeth

and
dental
implants;
middle
ear;
gastrointes7nal
tract;

urogenitaltract;
airway/lung
7ssue;
eye;
urinary
tract
prostheses;

peritoneal
membrane
and
peritoneal
dialysis
catheters,
in‐dwelling

catheters
for
hemodialysis
and
for
chronic
administra7on
of

chemotherapeu7c
agents
(Hickman
catheters);
cardiac
implants

such
as
pacemakers,
prosthe7c
heart
valves,
ventricular
assist

devices,
and
synthe7c
vascular
grads
and
stents;
prostheses,

internal
fixa7on
devices,
percutaneous
sutures;
and
tracheal
and

ven7lator
tubing.


11
 How
is
Biofilm
formed?

5 stages of biofilm development.

•  Stage
1,
ini7al
ahachment;
stage
2,
irreversible
ahachment;
stage

3,
matura7on
I;
stage
4,
matura7on
II;
stage
5,
dispersion.

•  Each
stage
of
development
in
the
diagram
is
paired
with
a

photomicrograph
of
a
developing
P.
aeruginosa
biofilm.


12





Nature:
May
2002

 Where do they grow?
• Biofilm formation appears common near polluted and toxic areas
and environments.

•  Account for the majority of all microbial infections of the human

body.

•  Device-related infections,
intravenous catheters,
joint prostheses, braces,
Root canal posts, implants, metal
fillings.

• Human body –
Mouth, teeth (perio, gingivitis),
Pancreatic-biliary tracts, lungs,
sinuses, adenoids, tonsils and
the intestinal tract/bowel….

13
 Why are they so difficult to treat?
• Remarkably difficult to treat with antimicrobials, resistant to
doses of antimicrobials 100- to 1000-fold over the minimum
lethal dose for microbes outside of biofilms.
• Antibiotics do not penetrate the polysaccharide matrix.
• Highly resistant to both immunological and non-specific
defense mechanisms of the body.
• Difficult to diagnose, difficult to culture.
• Microbes impart genetic material to one another to maintain
resistance.
• Colonies communicate with one another through the use of
quorum sensing molecules.
• Colonies fail to express OMP (outer membrane proteins).

“Testing the Susceptibility of Bacteria in Biofilms to Antibacterial Agents” Antimicrobial Agents

14
and Chemo. Nov 1990.
Autism (ASD)

Many
pa7ents
with
au7s7c
symptoms
have
persistent
dysbiosis.

Treatment
of

GI
issues
oden
alleviates
the
symptoms
we
call
au7sm.


Pa7ents
with
au7sm
along
with
toxic
metal
burdens
and
toxic
chemical
burdens

are
likely
to
grow
resistant
organisms
in
their
GI
tract.
This
resistance
to

treatment
is
perpetuated
by
the
produc7on
of
biofilms.

Treatment
of
biofilms

will
help
to
eradicate
dysbio7c
flora
helping
rid
symtpoms.


15
 Quorum
Sensing

bacteria
communicate
with
each
other
via
signal
molecules
or
autoinducers
 

Autoinducers
drive
bacterial

gene
expression
and
regulate
 QS
signals
can
be
blocked


–  Bioluminescence
 •  Gram
nega7ve
bacteria
produce

–  Virulence
 lactones

–  Biofilm
 •  E.
coli
produce
epinephrine

–  Conjuga7on
 –  Brominated
furanones
(marine

algae)
are
quorum
sensing

–  Mo7lity
 inhibitors

–  Presence
of
Normal
Bacteria
 •  Gram
posi7ve
bacteria
produce

cyclic
pep7des

–  RIP,
a
synthe7c
pep7de,

interferes
with
biofilm

infec7ons
in
animal
models


“Cell to Cell Signaling in Intestinal Pathogens” Curr Issues Intest Microbiol,Mar 2004
16
“Bacteria-Host Communication: The Language of Hormones”Microbiology. May 2003
 Is
it
possible
my
child
has
Biofilm

producing
bacteria
and
yeast
overgrowth?







•  Consider
if

–  Persistent
/Recurrent
Dysbiosis

–  Recurrent
Sinusi7s/O77s

–  Ini7ally
does
well
with
An7bio7cs
and
An7fungals

–  History
of
frequent
An7bio7cs

–  Frequent
flaring
of
yeast/bacteria
during
DETOX

–  Concomitant
–
gingivi7s,
tonsilli7s,
dental
caries

–  Stool
and
Urine
Cultures
nega7ve,
but
pa7ent
does
well
when
placed

on
an7fungals/an7bio7cs


–  SAME
CONSIDERATION
FOR
ADULTS.


17
 Microbial
biofilms
in
the
human

gastrointes7nal
tract.

J
Appl
Microbiol.
2007
May;102(5):1187‐96.


Macfarlane
S,
Dillon
JF.


There
is
moun7ng
interest
in
mucosal
biofilms
in
the
colon,

especially
with
respect
to
their
role
in
inflammatory
bowel

disease.
Because
bacteria
growing
in
biofilms
are
more
resistant

to
an7bio7cs
than
unahached
organisms,
it
is
oden
difficult
to

modify
the
structure
and
composi7on
of
these
communi7es,
or

to
eradicate
them
from
the
body.



However,
recent
work
has
shown
that
there
is
considerable

poten7al
to
alter
the
species
composi7on
of
mucosal
biofilms
in

a
beneficial
way
using
synbio7cs.


18
 Proteoly?c
enzymes:
a
new
treatment
strategy
for

prosthe?c
infec?ons? 

An7microb
Agents
Chemother.
1993
Dec

Selan
L,
BerluG
F,
Passariello
C,
Comodi‐Ballan?
MR,
Thaller
MC.


Among
the
different
mechanisms
of
bacterial
resistance
to
an7microbial
agents

that
have
been
studied,
biofilm
forma7on
is
one
of
the
most
widespread.
This

mechanism
is
frequently
the
cause
of
failure
in
the
treatment
of
prosthe7c

device
infec7ons,
and
several
ahempts
have
been
made
to
develop
molecules

and
protocols
that
are
able
to
inhibit
biofilm‐embedded
bacteria.
We
present

data
sugges7ng
the
possibility
that
proteoly7c
enzymes
could
significantly

enhance
the
ac7vi7es
of
an7bio7cs
against
biofilms.


An7bio7c
suscep7bility
tests
on
both
planktonic
and
sessile
cultures,
studies
on

the
dynamics
of
coloniza7on
of
10
biofilm‐forming
isolates,
and
then

bioluminescence
and
scanning
electron
microscopy
under
seven
different

experimental
condi7ons
showed
that
serra?opep?dase
greatly
enhances
the

ac7vity
of
ofloxacin
on
sessile
cultures
and
can
inhibit
biofilm
forma7on.


19
 Enhancement
of
the
Fibrinoly?c
Ac?vity
in

Plasma
by
Oral
Administra?on
of
NaOokinase.

Acta
Haematol.
1990;84(3):139‐43


Sumi
H,
Hamada
H,
Nakanishi
K,
Hiratani
H.

The
existence
of
a
potent
fibrinoly7c
enzyme
(nahokinase,
NK)
in
the

tradi7onal
fermented
food
called
'naho',
was
reported
by
us
previously.
It

was
confirmed
that
oral
administra7on
of
NK
(or
naho)
produced
a
mild

and
frequent
enhancement
of
the
fibrinoly7c
ac7vity
in
the
plasma,
as

indicated
by
the
fibrinoly7c
parameters,
and
the
produc7on
of
7ssue

plasminogen
ac7vator.



NK
capsules
were
also
administered
orally
to
dogs
with
experimentally

induced
thrombosis,
and
lysis
of
the
thrombi
was
observed
by

angiography.
The
results
obtained
suggest
that
NK
represents
a
possible

drug
for
use
not
only
in
the
treatment
of
embolism
but
also
in
the

preven7on
of
the
disease,
since
NK
has
a
proven
safety
and
can
be
mass

produced.


20
 Three
Types
of
Enzymes

•  There
are
three
basic
types
of
enzymes
needed
to

sustain
life.
Two
of
these
are
produced
within
the
body,

the
third
is
not,
and
must
be
provided
in
the
foods
we

eat.

•  
The
three
types
of
enzymes
are
metabolic
enzymes,

diges7ve
enzymes,
and
food
enzymes.
Metabolic
and

diges7ve
enzymes
perform
a
host
of
func7ons
while

food
enzymes
have
a
more
limited
purpose.
Food

enzymes
are
needed
in
the
diges7ve
process,
but

generally
only
serve
to
help
digest
the
par7cular
type

of
food
they
are
present
in.


21
 Enzymes
Role

•  Enzymes
are
proteins
that
catalyze
(i.e.,

increase
the
rates
of)
chemical
reac7ons.[1][2]
In

enzyma7c
reac7ons,
the
molecules
at
the
beginning
of

the
process
are
called
substrates,
and
the
enzyme

converts
them
into
different
molecules,
called
the

products.


•  Almost
all
processes
in
a
biological
cell
need
enzymes

to
occur
at
significant
rates.
Since
enzymes
are

selec7ve
for
their
substrates
and
speed
up
only
a
few

reac7ons
from
among
many
possibili7es,
the
set
of

enzymes
made
in
a
cell
determines
which

metabolic
pathways
occur
in
that
cell.


22
 BioFilm
Detox

Proprietary
Formula
includes:

•  Xylanase
is
the
name
given
to
a
class
of
enzymes
which
degrade
the
linear

polysaccharide
beta‐1,4‐xylan
into
xylose[1],
thus
breaking
down
hemicellulose,
one

of
the
major
components
of
plant
cell


•  Glucoamylase:Any
enzyme
that
hydrolyzes
the
glucoside
bond
in
starches
and

dextrins

•  Alpha‐galactosidase
is
a
glycoside
hydrolase
enzyme
that
hydrolyses
the
terminal

alpha‐galactosyl
moie7es
from
glycolipids
and
glycoproteins.
It
is
encoded
by
the

gene.

•  Pep7dase:
A
proteoly7c
enzyme
which
breaks
up
small
proteins
in
the
endosperm
to

form
amino
acids.

•  Lipase:

an
enzyme
secreted
in
the
diges7ve
tract
that
catalyzes
the
breakdown
of

fats
into
individual
fahy
acids
that
can
be
absorbed
into
the
bloodstream

•  Beta‐glucanase:
enzyme
which
breaks
down
beta‐glucans,
which
hold
together

branced
starch
molecules.


•  Cellulase
refers
to
a
class
of
enzymes
produced
chiefly
by
fungi,
bacteria,
and

protozoans
that
catalyze
the
cellulolysis
(or
hydrolysis)
of
cellulose.
However,
there

are
also
cellulases
produced
by
other
types
of
organisms
such
as
plants
and

animals.
...

•  Invertase:

an
enzyme
that
catalyzes
the
hydrolysis
of
sucrose
into
glucose
and

fructose

23
 BioFilm
Detox


•  Bromelain
can
refer
to
one
of
two
protease
enzymes
extracted
from

the
plant
family
Bromeliaceae,
or
it
can
refer
to
a
combina7on
of

those
enzymes
along
with
other
compounds
produced
in
an

extract.

•  Amylase
is
an
enzyme
that
breaks
starch
down
into
sugar.
Amylase

is
present
in
human
saliva,
where
it
begins
the
chemical
process
of

diges7on.

•  Pec7nase:

Any
enzyme
that
breaks
down
pec7n,
a
polysaccharide

substrate
found
in
the
cell
wall
of
plants,
into
simple
sugars
and

galacturonic
acid.


•  Nahokinase:

Nahokinase,
a
sub7lisin‐like
serine
proteinase

produced
by
Bacillus
na>o,
is
present
in
the
tradi7onal
fermented

Japanese
soyabean
product
"naho".


Used
for
blood
clots,

circula7on,
athletes,
cardiovascular
system
and
inflamma7on.

•  Brown
Algae:
(fucoiden)


24
 The
Miracle
Enzyme

•  Serratiopeptidase (Serratia E-15 protease, also known as serralysin,
serratiapeptase, serratia peptidase, serratio peptidase, or serrapeptidase)
is a proteolytic enzyme (protease) produced by enterobacterium.
•  Fortunately, there is a natural alternative to steroids and NSAIDs that is
effective without serious side effects. Serratiopeptidase or serrapeptase is
a protein (proteolytic) enzyme isolated from the non-pathogenic
enterobacteria Serratia E15 found in silkworms.
•  It has been used successfully for almost 40 years in Japan and Europe for
pain and inflammation due to arthritis, trauma, surgery, sinusitis,
bronchitis, carpal tunnel and painful swelling of the breasts. Keyword:
Inflammation & Pain
•  May be useful for atherosclerosis as well. This enzyme is absorbed
through the intestines and transported directly into the bloodstream.
PROBLEM
•  It is true that Serratiopeptidase neesd to be enteric coated to pass through
the gut or it will be destroyed. HOWEVER, Enteric coated tablets are toxic.
SOLUTION
Therefore, The SERRATIOPEPTIDASE we use is enteric coated, can be used in
capsules and works great.
25
 Supplement Facts
Serving Size: 2 Capsules
Servings Per Container: 60

120 veggie caps caps per bottle Amount per Serving % DV

Serratiopeptidase (120,000 SU/gram) 480 mg *

Proprietary Enzyme Blend 437 mg *
glucoamylase
alpha galactosidase
bromelain
cellulase
beta glucanase
fungal amylase
Fungal protease II
Peptidase AO
invertase
lipase
pectinase
peptidase
xylanase
Nattokinase (20,000 FU/gram) 20 mg
Brown Algae (Fucus vesiculosus) Fucoiden 300 mg *
* Daily Value not established
Other Ingredients: Cellulose (capsule) 26
BioFilm
Detox


27
 1.

Protocol‐
BioFilm
Detox
(BFD)




Children:

1
per
day
for
children
with
ASD
or
other
gut/immune
issue
(before
a

meal).




Adults:

•  1
two
to
three
7mes
per
day,
before
meals.
(label
says
two
daily)

•  1
Digest
Pro
(herbs)
with
each
meal.

•  Then
30
minutes
ader
ea7ng
use
the
BioTox
Nosodes,
Bowel

Nosodes,
Candida‐Ease
or
anything
to
kill
microbes.



•  Remember,
nosodes
are
used
to
nutralize
bacteria/virus/pathology.


They
are
homeopathically
prepared
from
pathology.


•  Use
the
Biofilm
Detox
15
minutes
before
taking
the
above

homeopathic
nosodes
to
eliminate
biofilm
so
that
the
nosodes
will

be
more
effec7ve.

BioFilm
Defense
is
taken
with
water.


28
 2. MORE Biofilm Control Strategies

•  Optimal Multi Pro, Nano Ionic Multi with Silica are necessary.
•  Additional nutrients for each individual symptomology are
necessary along with BioTox Nosodes, Bowel Nosodes or
Candida-Ease.
•  BioFilm Detox is taken 15 minutes before any homeopathic
remedy. It is taken with water.Probiotics and Prebiotics
•  Digest Pro - mucous degrading enzyme support.
•  Prebiotics, Probiotics are necessary in small amounts to
prevent the need for antibiotics; antibiotics will kill all the good
bacteria ie weakens immune system..

29
 3.

Gut
Biofilm
Protocol



•  Step
1
Detachment:
2
weeks
to
one
month

–  BioFilm
Detox
(immediately
before
two
or
three
meals
daily),
Slow

Cleanse,
Digest
Pro
(1
with
each
meal)


•  Step
2
Microbial
Killing:
two
month

–  BioFilm
Detox,
Slow
Cleanse,
Digest
Pro,


–  Homeopathic
Medicine:
Biotox
Nosodes,
Bowel
Nosodes,
Candida‐
Ease,
Lymph
Detox.
See
label
or
individually
test
pa7ent.


–  Watch
for
die
off
and
treat
with
Lymph
Detox,
Ocean
Med’s.


•  Step
3
Clean
up
and
Rebuilding/Nourishing
the
Gut
Lining:

one
month

–  Ocean
Med’s
(Fucoiden
from
brown
algae)

–  Fiber,
insoluble/soluble

–  Probio7cs

–  Prebio7cs

–  Fermented
Foods

–  Healing,
nutri7ous,
organic
non‐toxic
foods

–  Suppor7ve
Nutrients

30
 Probio7cs
–
beneficial
bacteria

•  Bifidobacterium
(breve,
longum,
infan7s)

•  Lactobacillus
(acidophilus,
casei,
paracasei,

johnsonii,
lac7s,
plantarum,
reuteri,
rhamanosus,

salivarius
)

•  And….


31
 An7‐oxidant
rich
foods


help
reduce
inflamma7on

  Inadequate
an7oxidant
status
is
a
major
pathway
for
inflamma7on.



  Various
free
rdicals
(ROS),
including
superoxide,
peroxide,
hydroxyl
and

peroxynitrite,
are
generated
through
the
inflammatory
prostaglandin/
leukotriene
pathways.


  These
free
radicals
can
damage
or
destroy
virtually
every
cellular
biomolecule:

proteins,
fahy
acids,
phospholipids,
glycoproteins,
even
DNA,
leading
to
cell

injury
or
death.




  Vitamin
C,
E,
alpha‐lipoic
acid,
Co
Q10
and
NADH
act
as
a
team.

(Op?mal

Mul?
Pro)


  One
of
the
many
ways

pro‐oxidants
damage
neurons
is
to
prevent
the

intracellular
forma7on
of
glutathione.

Therefore
our
formula
Hepathic

Glutathione
Pathway
formula
should
be
used
in
the
the
Repair
phase.


32
•  Thank
you.


33