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Citric Acid Cycle - Overview of the Citric Acid Cycle

An important energy-generating metabolic pathway, also called the Krebs cycle (after the German-born
British biochemist H. A. Krebs who identified it) or tricarboxylic acid cycle; it forms the final stage in the
oxidation of carbohydrates, fats, and proteins in cells. The citric acid cycle occurs inside the mitochondria of
cells and is capable of producing enough ATP (adenosine triphosphate) to run all of the cell's functions.

The citric acid cycle begins with a glucose molecule, which during the process of glycolysis is stripped of
some of its hydrogen atoms, transforming the glucose into two molecules of pyruvic acid. Next, pyruvic
acid is altered by the removal of a carbon and two oxygens, which go on to form carbon dioxide. When the
carbon dioxide is removed, energy is given off, and a molecule called NAD+ is converted into the higher
energy form, NADH. Another molecule, coenzyme A (CoA), then attaches to the remaining acetyl unit,
forming acetyl coenzyme A (acetyl CoA).

Acetyl CoA enters the citric acid cycle by joining to a four-carbon molecule called oxaloacetate. Once the
two molecules are joined, they make a six-carbon molecule called citric acid. Citric acid is then broken
down and modified in a stepwise fashion. As this happens, hydrogen ions and carbon molecules are
released. The carbon molecules are used to make more carbon dioxide. The hydrogen ions are picked up by
NAD and another molecule called flavin-adenine dinucleotide (FAD). Eventually, the process produces the
four-carbon oxaloacetate again, ending up where it started off.

All in all, the citric acid cycle is capable of generating from 24 to 28 ATP molecules from one molecule of
glucose converted to pyruvate. Therefore, it is easy to see how much more energy we can get from a
molecule of glucose if our mitochondria are working properly and if we have oxygen.