Application: Tecan i-control Tecan i-control , 1.5.14.

0
Device: infinite 200 Serial number: 901006981 Serial number of connected stacker:
Firmware: V_2.11_04/08_InfiniTe (Apr 4 2 MAI, V_2.11_04/08_InfiniTe (Apr 4 2008/14.37.11)

Date: ###
Time: 14:02:06

System FM-144598422
User FM-144598422\Administrator
Plate Nunclon 96 Flat Bottom Transparent Polystyrol [NUN96ft.pdfx]
Plate-ID (Stacker)

Label: Label1
Mode Absorbance
Multiple Reads per Well (Circle (filled)) 7x7
Multiple Reads per Well (Border) 200 µm
Wavelength 560 nm
Bandwidth 9 nm
Number of Flashes 5
Settle Time 0 ms
Start Time:2011/02/12 14:02:06

Multiple Reads Per Well -

Alignment

11 1
111 1 1
1111 1 11
1111 1 11
1111 1 11 1
111 1 1 1
11 1
Well Mean StDev 2;6 3;6 4;6 5;5 4;5 3;5 2;5
A1 0.3845 0.0325 0.4224 0.3595 0.3566 0.4304 0.4011 0.3732 0.382
A2 0.4358 0.0322 0.4424 0.4359 0.4093 0.4157 0.4573 0.4154 0.4596
A3 0.425 0.0334 0.4214 0.4012 0.3978 0.4083 0.4304 0.3924 0.3991
A4 0.3691 0.03 0.3447 0.3399 0.3593 0.3916 0.3436 0.317 0.3523
A5 0.3932 0.0377 0.3627 0.3744 0.393 0.4368 0.3729 0.3312 0.365
A6 0.4126 0.0349 0.4638 0.4336 0.3671 0.375 0.3721 0.3545 0.4332
A7 0.5402 0.032 0.5472 0.5251 0.5214 0.4967 0.5494 0.5197 0.5471
A8 0.4735 0.0426 0.3868 0.3655 0.4013 0.453 0.4648 0.3972 0.3952
A9 0.4375 0.0451 0.4289 0.4375 0.47 0.4864 0.4777 0.4071 0.3515
A10 0.4584 0.0286 0.4263 0.3993 0.4521 0.4806 0.4605 0.4202 0.4558
A11 0.5051 0.03 0.4943 0.4641 0.5006 0.4895 0.4999 0.476 0.5084
A12 0.4065 0.0239 0.4037 0.3764 0.3802 0.4312 0.4093 0.3821 0.4042
B1 0.7478 0.0451 0.732 0.8318 0.821 0.7935 0.7634 0.7103 0.7948
B2 0.8666 0.0575 0.9682 0.9604 0.9537 0.9306 0.8675 0.837 0.886
B3 0.6628 0.0588 0.7784 0.781 0.7745 0.7765 0.7006 0.638 0.6749
B4 0.6246 0.0471 0.7161 0.7336 0.7699 0.668 0.6259 0.6005 0.6321
B5 0.8931 0.0451 1.0031 0.9581 0.988 0.9308 0.8876 0.8654 0.9077
B6 0.5791 0.0521 0.6607 0.6823 0.7144 0.6964 0.6154 0.5316 0.5411
B7 0.9944 0.1027 1.1625 1.2093 1.1483 1.0405 1.0408 1.0137 1.0496
B8 0.7875 0.0791 0.9518 0.9786 0.9788 0.8923 0.858 0.801 0.8017
B9 0.7362 0.0783 0.9135 0.9281 0.9342 0.8333 0.8139 0.7069 0.7091
B10 0.8362 0.1705 1.1159 1.1515 1.123 1.0312 0.9119 0.809 0.8301
B11 0.7872 0.0731 0.9125 0.9372 0.9387 0.9409 0.8484 0.748 0.765
B12 0.6895 0.0503 0.803 0.7008 0.5726 0.6075 0.6813 0.6164 0.7065
C1 0.9805 0.0502 0.866 1.0978 1.1084 1.0624 1.0063 0.9947 0.9987
C2 1.0315 0.0514 1.0927 1.0914 1.0818 1.0168 1.0234 0.9987 1.0102
C3 1.0466 0.0516 1.128 1.0794 1.0649 1.0562 0.9934 1.0002 1.0651
C4 1.043 0.0488 1.1087 1.0759 1.1462 1.1355 1.0586 0.9651 0.9935
C5 1.0422 0.0707 1.1134 1.2075 1.2256 1.1561 1.1644 1.0624 1.0633
C6 1.1027 0.1179 1.3008 1.3335 1.4933 1.2538 1.2213 1.073 1.066
C7 1.0272 0.1177 1.2445 1.3821 1.391 1.1338 1.115 1.0095 1.0211
C8 0.9788 0.0981 1.1799 1.2051 1.2322 1.1319 1.0185 0.9141 0.9099
C9 1.0365 0.1099 1.274 1.304 1.2803 1.2348 1.0848 0.9793 0.9643
C10 0.9936 0.1166 1.2436 1.2787 1.2867 1.1756 1.0398 0.9524 0.9477
C11 1.056 0.1086 1.1522 1.2382 1.2539 1.3335 1.083 0.9881 0.9698
C12 0.9885 0.0972 1.1584 0.9704 0.7745 0.8057 0.9618 0.9732 1.0125
D1 1.0684 0.0662 0.9925 1.2431 1.1521 1.0958 1.0951 1.0889 1.0832
D2 1.064 0.0565 1.1694 1.1847 1.1589 1.0781 1.0413 1.1032 1.1528
D3 1.0804 0.0555 1.1257 1.1075 1.1196 1.0905 1.0375 1.0116 1.0258
D4 1.0244 0.0549 1.1472 1.2007 1.1086 1.0771 1.0565 1.0391 1.0329
D5 1.1123 0.0946 1.3052 1.2974 1.2836 1.2399 1.2399 1.1386 1.1624
D6 1.103 0.0641 1.2031 1.239 1.2494 1.1335 1.1402 1.036 1.0512
D7 1.0302 0.1163 1.2849 1.3204 1.2555 1.1457 1.1321 1.0665 1.0899
D8 0.9517 0.0978 1.11 1.1122 1.1325 1.0808 1.0855 0.9341 0.8873
D9 0.9152 0.0751 1.0627 1.0934 1.0927 1.019 1.0113 0.8971 0.8813
D10 1.0444 0.0719 1.1796 1.1631 1.1891 1.1317 1.1028 1.0026 1.0396
D11 0.9802 0.1076 1.2246 1.2296 1.1685 1.148 1.0578 0.9626 1.0171
D12 1.0204 0.079 1.1612 1.0145 0.8063 0.7909 1.0001 1.0361 1.0304
E1 1.1455 0.0666 0.9716 1.195 1.2091 1.2945 1.1911 1.131 1.1623
E2 1.0465 0.0743 1.215 1.2038 1.1143 1.0285 0.9751 1.0197 1.0365
E3 1.1312 0.0646 1.1356 1.0653 1.0686 1.0912 1.0268 1.0071 1.1035
E4 1.0782 0.052 1.0738 1.1442 1.1865 1.1188 1.1178 1.0377 1.0345
E5 1.0674 0.1076 1.3048 1.264 1.2486 1.18 1.2057 1.1463 1.1963
E6 1.1262 0.0781 1.2688 1.261 1.2383 1.2107 1.2177 1.1084 1.1331
E7 0.9933 0.0786 1.119 1.1759 1.1349 1.1289 1.0942 0.9989 0.9618
E8 0.9722 0.1108 1.1605 1.1806 1.1666 1.1383 1.1192 0.9944 0.9759
E9 0.9814 0.0961 1.154 1.1633 1.1624 1.1764 1.0708 0.9608 0.9493
E10 1.0353 0.0803 1.1787 1.1692 1.1865 1.1549 1.1012 1.0381 0.9808
E11 1.04 0.063 1.1289 1.1147 1.0853 1.1687 1.1116 1.0025 1.0006
E12 0.9919 0.085 1.111 0.9673 0.8238 0.8351 1.1013 1.0576 0.9797
F1 1.0856 0.0585 0.914 1.1714 1.1691 1.1694 1.1305 1.0877 1.0609
F2 1.0945 0.0592 1.1934 1.1298 1.0908 1.1229 1.0581 1.0503 1.1007
F3 1.1919 0.0536 1.2666 1.2035 1.1517 1.1468 1.1018 1.1293 1.2185
F4 1.0428 0.0548 1.157 1.0885 1.1178 1.1056 1.0991 1.0065 1.0436
F5 1.0483 0.0671 1.1541 1.2094 1.1652 1.1194 1.0761 1.0265 1.0411
F6 1.1982 0.0542 1.2247 1.1736 1.2566 1.2753 1.2753 1.14 1.1157
F7 1.101 0.1081 1.2695 1.2963 1.3056 1.2617 1.2248 1.0997 1.0074
F8 0.984 0.1047 1.1055 1.2067 1.1536 1.0946 1.0524 0.9844 0.962
F9 0.9293 0.1087 1.1498 1.1833 1.1843 1.0036 1.0709 0.97 0.8913
F10 0.9653 0.0824 1.0521 1.1568 1.0993 1.1271 1.0549 1.0111 0.9686
F11 0.9646 0.0743 1.0006 1.1109 1.0598 0.9857 0.9987 0.8607 0.8857
F12 1.1952 0.1155 1.426 1.1347 0.8982 0.9453 1.2434 1.3275 1.255
G1 1.0579 0.0433 0.9397 1.1322 1.1131 1.1147 1.0756 1.0224 1.051
G2 1.0288 0.0564 1.1294 1.1428 1.1533 1.1447 1.0829 1.024 0.9674
G3 1.0052 0.0634 1.1261 1.1127 1.0749 1.061 1.0012 0.9761 1.0565
G4 1.0599 0.0566 1.1151 1.0945 1.0674 1.0992 0.9847 1.001 1.0996
G5 1.0754 0.0604 1.189 1.2074 1.1809 1.1932 1.1236 1.0902 1.1522
G6 1.126 0.0633 1.2596 1.1998 1.2809 1.1377 1.1491 1.0746 1.0478
G7 1.1291 0.0937 1.2711 1.3027 1.285 1.1927 1.2891 1.1376 1.144
G8 0.9131 0.0822 0.971 1.0866 1.0301 1.0746 1.0243 0.9304 0.8616
G9 1.0208 0.0777 1.0572 1.0962 1.1073 1.0994 1.1185 0.92 0.9369
G10 0.8947 0.0715 0.9807 0.958 0.9703 0.9715 0.9025 0.8305 0.801
G11 0.8937 0.0748 1.0418 0.9873 0.931 0.856 0.892 0.8188 0.879
G12 0.9101 0.074 1.0751 0.8997 0.7599 0.7663 0.9302 0.9161 0.9239
H1 0.9228 0.0494 0.8998 1.0242 1.0108 1.0141 0.9747 0.9128 0.9406
H2 1.0206 0.0699 1.1384 1.1269 1.099 1.1609 1.0737 1.025 1.0143
H3 1.135 0.055 1.1431 1.1982 1.1997 1.2423 1.1545 1.0389 1.0531
H4 1.064 0.0559 1.1732 1.1303 1.0638 1.1619 1.1079 0.9921 1.0271
H5 1.1915 0.0719 1.1933 1.2316 1.1991 1.2352 1.1856 1.1058 1.1039
H6 1.1654 0.0833 1.3383 1.2782 1.1831 1.2872 1.1758 1.0711 1.145
H7 1.1049 0.0719 1.1261 1.1471 1.1678 1.1928 1.1499 1.0504 1.0207
H8 1.0277 0.0687 1.1549 1.1463 1.1698 1.1202 1.113 1.0122 1.0545
H9 0.9559 0.065 1.1208 1.0677 0.9392 0.9677 0.9653 0.8888 0.9417
H10 1.0547 0.0601 1.1722 1.2066 1.125 1.1261 1.0922 1.0979 1.0787
H11 1.1029 0.0905 1.109 1.2069 1.1436 1.4075 1.1827 1.0742 0.9685
H12 0.9929 0.0748 1.1875 1.0457 0.8684 0.8366 1.0583 1.0888 1.0187

End Time: 2011/02/12 14:12:52

Movement Move Plate Out

mber of connected stacker:
1;5 0;4 1;4 2;4 3;4 4;4 5;4 6;4 6;3 5;3
0.4076 0.4174 0.3721 0.3878 0.4121 0.4123 0.4273 0.4612 0.4105 0.3646
0.465 0.4014 0.4121 0.4111 0.3894 0.4269 0.4243 0.45 0.4774 0.4374
0.3881 0.4781 0.4479 0.4071 0.4065 0.4281 0.4412 0.4893 0.466 0.3921
0.3511 0.3722 0.3913 0.3422 0.3541 0.3549 0.4142 0.4326 0.4064 0.3769
0.4418 0.422 0.3569 0.36 0.332 0.3243 0.385 0.3668 0.3634 0.3769
0.4256 0.3723 0.4768 0.4552 0.411 0.3984 0.4092 0.376 0.403 0.3968
0.5674 0.5263 0.5445 0.5254 0.533 0.5298 0.5392 0.5006 0.5602 0.5398
0.4328 0.4959 0.4944 0.4705 0.4655 0.467 0.488 0.4828 0.5055 0.4937
0.4344 0.4073 0.4109 0.3955 0.4122 0.4232 0.4786 0.5707 0.5365 0.4705
0.481 0.4732 0.4409 0.4736 0.4775 0.427 0.4495 0.4052 0.4248 0.423
0.5664 0.5552 0.5008 0.4876 0.4696 0.471 0.5018 0.4853 0.4666 0.4845
0.4012 0.3998 0.3617 0.3793 0.3773 0.3846 0.396 0.4066 0.3622 0.3884
0.7993 0.813 0.7743 0.8009 0.7432 0.723 0.8003 0.7993 0.7351 0.7176
0.9526 0.9378 0.8951 0.8712 0.8488 0.8252 0.8592 0.8826 0.9065 0.8303
0.755 0.6804 0.6252 0.634 0.6318 0.6553 0.6941 0.7477 0.7115 0.6227
0.6863 0.664 0.6035 0.6027 0.5957 0.5835 0.621 0.6822 0.6387 0.5925
0.9126 0.8963 0.8454 0.8882 0.8735 0.8639 0.8968 0.982 0.903 0.8798
0.601 0.5383 0.5097 0.5715 0.5817 0.5765 0.6091 0.6255 0.5953 0.5892
1.1566 1.2104 0.9882 0.9961 0.9391 0.8657 0.9974 1.1149 1.0407 0.9383
0.8548 0.7923 0.7195 0.7629 0.7728 0.7318 0.8386 0.9097 0.8502 0.7518
0.8368 0.7937 0.6861 0.6647 0.6898 0.7138 0.7765 0.8583 0.7464 0.7137
0.9142 1.0248 0.7231 0.6893 0.6787 0.7175 0.9139 1.1171 1.098 0.8625
0.8775 0.8534 0.7198 0.7324 0.7429 0.6995 0.8047 0.8477 0.8596 0.7517
0.7755 0.7575 0.648 0.6147 0.6365 0.6669 0.7372 0.6443 0.6436 0.7148
0.9933 0.9908 0.9563 0.9692 0.957 0.9283 0.9678 1.0668 1.0195 0.902
1.0506 1.1696 1.0783 1.0536 1.051 0.9678 0.9648 0.9598 0.9527 0.9413
1.1573 1.1348 1.1163 1.0897 1.0381 1.0211 1.0545 1.0806 1.0745 1.0267
1.1196 1.1395 1.0295 0.9762 1.0171 1.025 1.0602 1.0324 1.0412 1.0125
1.086 0.9981 0.9601 1.0222 1.0484 1.0563 1.1224 1.1262 1.0521 1.0475
1.2003 1.2015 0.9735 0.989 1.0322 1.034 1.1353 1.2299 1.185 1.0851
1.1165 1.1161 0.9505 0.936 0.942 0.95 1.0716 1.1234 1.1046 1.0143
1.0711 1.0439 0.868 0.8389 0.8315 0.8886 1.0251 1.0664 1.0244 0.9719
1.1215 1.0308 0.8825 0.9041 0.9264 0.9521 1.0768 1.2003 1.086 1.0088
1.0679 1.0106 0.8586 0.8608 0.8662 0.9008 1.0518 1.1468 1.1114 0.9415
1.067 1.0645 0.9394 0.9341 0.9068 0.9787 1.1205 1.2958 1.2248 1.0443
1.1253 1.16 0.988 0.9529 0.9044 0.9189 0.9838 0.7797 0.8094 0.9635
1.1106 1.1162 1.0813 1.0475 1.0092 1.0258 1.0775 1.1162 1.258 1.1002
1.1783 1.1231 1.0894 1.1327 1.112 1.046 1.0571 1.0681 1.0325 1.029
1.141 1.1179 1.1285 1.1293 1.1058 1.1258 1.0902 1.0641 0.965 0.9819
1.0703 1.0531 0.9975 1.0342 1.0129 0.9733 1.054 1.0542 1.0198 0.9856
1.2369 1.1333 1.0423 1.0904 1.1113 1.1576 1.2293 1.1786 1.0994 1.121
1.2065 1.1791 1.0395 1.046 1.0281 1.0136 1.1064 1.146 1.1287 1.0318
1.1711 1.0163 0.9191 0.9104 0.9404 0.9428 1.0668 1.1863 1.1517 1.0366
1.0021 0.9498 0.8232 0.8158 0.8651 0.9371 1.0578 1.0834 1.0694 0.9958
0.9685 0.9152 0.7774 0.8283 0.8637 0.8617 0.9207 0.9717 0.8992 0.8658
1.1221 1.0879 0.9745 0.9567 0.9805 0.9682 1.0162 1.1308 1.1367 0.9958
1.11 0.9809 0.8852 0.8795 0.8812 0.885 1.0279 1.1038 1.1169 0.8856
1.0626 1.0918 0.9788 0.999 1.0671 1.0542 1.0449 0.8512 0.8981 1.066
1.2012 1.1738 1.1639 1.1757 1.1972 1.2187 1.2332 1.1752 1.203 1.1784
1.0984 1.187 1.0662 0.996 0.9733 1.0049 1.0025 0.974 0.992 0.9816
1.1661 1.1692 1.1613 1.1784 1.1244 1.0909 1.0128 1.0334 1.035 1.0417
1.1186 1.2038 1.0979 1.0445 1.013 1.0274 1.0855 1.1007 1.0926 0.9898
1.2644 1.2267 1.089 1.0451 1.0922 1.0784 1.1061 1.1236 1.0806 1.04
1.2068 1.1704 1.0671 1.0745 1.0406 1.0737 1.214 1.2467 1.2109 1.141
1.0383 0.9984 0.8799 0.8889 0.9446 0.9567 1.0967 1.0687 1.0818 0.9867
1.0632 0.9631 0.8474 0.8925 0.9032 0.925 1.0658 1.1261 1.0688 0.9685
1.1124 1.0064 0.8471 0.8782 0.9066 0.9693 1.0968 1.0876 1.0384 1.0208
1.0707 1.0051 0.8896 0.9432 1.0143 0.992 1.0363 1.1463 1.1657 0.9964
1.0586 1.0121 0.8924 0.9763 0.9824 0.9498 1.065 0.9841 1.1328 0.9973
1.1062 1.1071 0.9944 0.9851 1.012 1.0617 1.0156 0.7966 0.8025 0.9603
1.114 1.0923 1.0491 1.0763 1.081 1.0801 1.1111 1.0417 1.0877 1.0972
1.2026 1.1765 1.1149 1.104 1.0331 1.0432 1.0483 1.0543 0.9887 0.9547
1.2701 1.195 1.2428 1.2641 1.2007 1.2091 1.1957 1.1768 1.1828 1.1833
1.1443 1.0688 1.0459 1.039 1.0209 0.9878 1.0869 1.0735 1.1254 1.0047
1.1475 1.0598 0.9856 1.0149 1.0045 0.9807 1.0615 1.1083 1.1154 1.0177
1.1784 1.1364 1.1256 1.2369 1.1954 1.1698 1.2484 1.2308 1.2393 1.1422
1.1365 1.1378 0.9652 0.9649 1.013 1.0634 1.1954 1.2446 1.3011 1.0717
1.0483 1.0583 0.9098 0.86 0.9072 0.9081 1.0304 1.1833 1.2121 1.0214
0.9714 0.9127 0.7887 0.8322 0.9252 0.891 0.9289 0.9524 1.007 0.8694
1.0509 1.0078 0.9132 0.9438 0.8896 0.8608 0.985 0.9718 1.0174 0.9136
0.9312 0.9679 0.8304 0.8758 0.8502 0.8945 1.0098 1.0112 1.1207 0.9497
1.3601 1.2113 1.1897 1.2401 1.2541 1.2402 1.3167 0.9748 0.9688 1.3026
1.0902 1.1328 1.0219 1.0008 1.0489 1.0594 1.0647 1.0678 1.1109 1.0399
1.0309 1.0357 0.9325 0.9384 1.0547 1.0561 1.059 0.9867 0.9754 1.0271
1.0556 1.1388 1.0441 1.02 0.9458 0.8953 0.941 1.0511 0.9919 0.9659
1.0721 1.0496 1.0713 1.0202 0.9757 0.9836 1.0606 0.9913 1.1106 1.0269
1.1345 1.056 1.0421 1.0844 0.9988 1.0435 1.0737 1.0761 1.1009 1.0313
1.1822 1.1567 1.0703 1.0704 1.0314 1.0842 1.1539 1.1097 1.1527 1.0972
1.2016 1.1911 1.0113 1.0129 1.1119 1.1039 1.2089 1.081 1.2001 1.0899
0.9879 0.977 0.8083 0.8367 0.8671 0.873 0.983 0.9782 0.9947 0.9077
1.0564 1.162 0.9485 0.9173 0.9326 0.9577 1.0385 1.0333 1.2112 0.9387
0.9768 1.0296 0.847 0.808 0.8018 0.7884 0.8673 0.9445 1.0398 0.8679
0.9767 0.927 0.8381 0.8625 0.8166 0.7561 0.8171 0.8668 0.9954 0.8322
1.0126 1.0353 0.9475 0.8927 0.8915 0.9118 0.8976 0.7477 0.7878 0.8914
0.9864 0.9882 0.9234 0.9162 0.9168 0.9097 0.9518 0.8987 1.0223 0.9101
1.0117 1.0452 0.9684 0.9924 1.0209 1.0679 1.1343 1.0756 1.0685 1.013
1.1282 1.1404 1.1178 1.0621 1.0801 1.0851 1.1391 1.066 1.1508 1.1161
1.0991 1.0471 1.0102 0.9827 1.0014 1.0456 1.1421 0.969 1.0474 1.0561
1.1307 1.2906 1.1724 1.1617 1.2006 1.1362 1.2208 1.0823 1.3121 1.2014
1.3235 1.2941 1.1611 1.0832 1.0977 1.1429 1.2309 1.1909 1.1755 1.1585
1.12 1.1273 1.034 1.0212 1.0388 1.0658 1.147 1.1182 1.313 1.1683
1.0639 1.0319 0.9883 0.9984 1.0178 1.0069 1.0508 1.0146 1.1622 1.0349
1.001 0.9947 0.8943 0.9276 0.868 0.8279 0.8679 0.8215 0.917 0.8821
1.0897 1.1055 1.0639 1.0624 1.0615 1.0232 1.0729 0.9595 1.0832 1.0373
1.0651 1.0638 0.9695 0.9905 1.0463 1.1583 1.2924 1.1122 1.2443 1.1659
1.0658 1.0444 0.9553 0.9813 1.0077 1.0114 0.9757 0.7868 0.8623 1.0678
4;3 3;3 2;3 1;3 0;3 0;2 1;2 2;2 3;2 4;2
0.3605 0.386 0.4057 0.3523 0.3332 0.3812 0.3519 0.4193 0.399 0.3829
0.3891 0.382 0.3944 0.4436 0.4055 0.4556 0.4814 0.4518 0.4368 0.4491
0.3915 0.4219 0.4093 0.4451 0.4421 0.3825 0.3901 0.3757 0.4547 0.4372
0.3436 0.3319 0.3558 0.4207 0.3854 0.4145 0.3884 0.3655 0.3529 0.3493
0.3902 0.3871 0.445 0.375 0.44 0.494 0.3914 0.3876 0.3757 0.3837
0.4169 0.4689 0.425 0.4193 0.3995 0.4678 0.3933 0.4171 0.4118 0.3935
0.5128 0.5094 0.5128 0.5422 0.5213 0.4934 0.5461 0.5059 0.5168 0.5252
0.474 0.4677 0.4757 0.5029 0.4694 0.5043 0.4728 0.5101 0.5141 0.505
0.4352 0.4498 0.4325 0.4493 0.4227 0.4376 0.4066 0.4321 0.4779 0.4513
0.4393 0.4467 0.4834 0.4651 0.4763 0.5081 0.4866 0.4995 0.4736 0.4467
0.5103 0.5081 0.4933 0.5005 0.531 0.5056 0.4534 0.4925 0.5149 0.5143
0.3966 0.4261 0.4156 0.4132 0.4245 0.4467 0.4224 0.413 0.4246 0.381
0.7102 0.7047 0.7154 0.7646 0.7916 0.7504 0.7706 0.7163 0.6816 0.7013
0.8136 0.8214 0.8312 0.8735 0.8908 0.9093 0.8568 0.7996 0.7656 0.8133
0.6239 0.6315 0.6139 0.6403 0.6498 0.6594 0.6221 0.592 0.5893 0.5919
0.6171 0.5938 0.6001 0.5886 0.6286 0.6696 0.5781 0.6007 0.6114 0.583
0.8602 0.8687 0.8564 0.8216 0.8332 0.8286 0.8185 0.8631 0.8912 0.9159
0.5749 0.5755 0.5783 0.5519 0.53 0.5539 0.5528 0.5904 0.5664 0.5403
0.8768 0.8747 0.8648 0.9278 1.0223 0.9645 0.8417 0.8666 0.875 0.8824
0.7525 0.7509 0.7482 0.7134 0.7798 0.7539 0.7082 0.7188 0.7733 0.7724
0.7242 0.7236 0.6634 0.6424 0.735 0.7192 0.6253 0.6583 0.6938 0.6923
0.6964 0.6494 0.6516 0.6784 0.9138 0.9029 0.6392 0.6337 0.6221 0.6701
0.7456 0.7472 0.7297 0.707 0.8051 0.8637 0.7314 0.7293 0.7293 0.7457
0.6959 0.679 0.6295 0.6399 0.7155 0.7418 0.6667 0.7061 0.7275 0.7065
0.9203 0.968 0.9802 0.952 0.915 0.9089 0.9537 1.0019 0.9741 0.98
1.0513 1.0508 1.0436 1.0078 1.0604 1.0374 1.0037 1.0403 1.1191 1.0837
1.0453 1.0698 1.0852 1.0962 1.0423 1.0365 1.0648 1.0639 1.0721 1.0116
1.052 1.0447 1.0533 1.0898 0.9878 1.1054 1.0808 1.0556 1.0788 1.002
1.056 1.0425 1.0158 0.9626 0.9737 0.9887 0.9527 1.03 1.0573 1.0487
1.0652 1.0051 0.9783 0.9386 1.0855 1.1227 0.9829 1.0242 1.0752 1.0828
0.972 0.9743 0.9203 0.9293 0.964 0.9187 0.912 0.9366 1.0111 1.0163
0.9229 0.8875 0.8975 0.9189 0.8803 0.9168 0.9178 0.9172 0.9768 0.9837
0.9951 1.0274 0.9764 0.8831 0.9326 0.8774 0.9361 1.0208 1.0537 1.0445
0.9408 0.9601 0.8871 0.8945 0.9606 0.9357 0.8819 0.9351 0.9703 0.9398
1.0228 1.0238 0.9728 0.8976 0.9667 0.9545 0.9224 0.9938 1.0685 1.0366
0.9359 0.9517 0.9927 1.0024 1.0299 0.9902 0.9964 1.0141 1.0734 1.0216
1.0716 1.0281 0.9863 0.9973 0.9611 1.0115 0.9812 0.9638 1.0539 1.0902
1.0086 1.0085 1.0387 1.0943 0.9606 1.0734 1.077 1.0265 0.9933 1.0106
1.1231 1.1492 1.1648 1.144 1.1067 1.1085 1.1135 1.1285 1.1137 1.0916
1.0181 1.036 1.0657 1.0069 0.9556 0.9607 0.9914 1.016 1.011 1.0551
1.1878 1.1184 1.0712 1.0152 1.0104 1.015 1.0088 1.0221 1.1005 1.0963
1.0636 1.1072 1.1225 1.058 1.0988 1.1028 1.0517 1.1494 1.133 1.0943
0.9493 0.9679 0.9279 0.8517 0.9266 1.0134 0.9313 1.0117 0.9987 0.9909
0.9595 0.927 0.8542 0.8201 0.8301 0.8758 0.8123 0.8319 0.9418 0.963
0.8598 0.9043 0.8805 0.8028 0.8301 0.8359 0.8322 0.8978 0.9222 0.897
0.9722 0.96 0.9935 0.9928 1.0165 1.0582 1.0175 0.9971 1.0318 0.9867
0.9186 0.9239 0.8746 0.8435 0.8473 0.8756 0.8685 0.9107 0.9911 0.9978
1.0758 1.0857 1.049 0.9576 0.9858 1.0271 1.0027 1.0416 1.1085 1.0646
1.1874 1.1631 1.1378 1.1267 1.0981 1.0313 1.0998 1.0998 1.1632 1.1639
1.0029 1.0085 1.0481 1.1418 1.1597 1.185 1.1648 1.0674 1.0356 1.011
1.1396 1.2045 1.1532 1.1536 1.1382 1.1569 1.2401 1.2683 1.2185 1.1423
1.0515 1.0579 1.0603 1.1053 1.1488 1.1233 1.0704 1.0634 1.0753 1.0303
1.0315 1.0364 1.014 1.0041 1.0255 1.0343 0.9745 1.0219 1.0762 0.9803
1.1038 1.0934 1.065 1.0291 1.0164 1.0617 1.0166 1.1004 1.0757 1.1575
0.9701 1.0074 0.9278 0.8745 0.9124 0.9273 0.9354 0.9696 1.0304 0.943
0.8971 0.9339 0.8967 0.8806 0.8539 0.913 0.8598 0.8941 0.9045 0.8943
1.0013 0.9617 0.953 0.8365 0.9024 0.9117 0.885 0.9458 0.9583 0.954
0.9929 1.0861 1.0658 0.9371 0.9468 0.9669 0.9501 1.0087 1.0373 0.9441
0.9891 0.9998 1.0403 0.9832 0.9362 1.057 1.0443 1.0647 1.0249 1.0257
1.041 1.0725 1.0191 0.9679 0.9527 0.9845 1.0134 1.0459 1.0968 1.0188
1.148 1.1385 1.0528 1.0375 1.0126 0.9912 1.0259 1.1214 1.1774 1.0943
1.0136 1.058 1.141 1.1428 1.1658 1.1485 1.0877 1.1283 1.105 1.0648
1.2151 1.2521 1.2235 1.2703 1.1806 1.2401 1.2649 1.2202 1.2142 1.1628
0.9712 1.0319 1.0213 1.0222 0.9718 0.9695 1.0453 1.0451 1.0781 1.0479
1.0381 1.0435 1.001 1.0332 0.9761 1.1162 1.0003 0.9825 1.1095 1.0239
1.1409 1.2772 1.2638 1.2032 1.073 1.1579 1.2195 1.2821 1.292 1.1925
1.1098 1.0636 1.0073 0.9366 1.047 1.0561 0.9815 1.0913 1.0703 1.0482
0.9331 0.927 0.9361 0.8691 0.9217 0.9321 0.8663 0.8548 0.9602 0.8863
0.8526 0.9382 0.8893 0.858 0.905 0.8925 0.8317 0.8448 0.9711 0.8727
0.8821 0.9367 0.9159 0.8363 0.9455 0.9379 0.8467 0.9847 0.9616 0.9512
0.939 0.9955 0.9793 0.8637 0.8666 0.949 0.9748 1.0148 1.0374 0.9318
1.2291 1.3079 1.2596 1.1867 1.1731 1.1405 1.1766 1.2274 1.2911 1.2064
1.0515 1.0465 1.0375 0.9976 1.0477 1.0344 1.0146 1.0127 1.0509 1.0815
1.0858 1.0692 1.0424 0.9729 1.0581 1.0318 1.0273 1.0243 1.0255 1.0067
0.9561 0.9675 1.0029 0.9908 1.036 1.0687 1.0181 1.0222 1.0939 0.9476
1.0762 1.124 1.1338 1.1206 1.0245 0.9875 1.0978 1.1078 1.1648 1.0874
1.0449 1.0697 1.0403 1.063 1.0101 0.9928 1.0551 1.0368 1.1487 1.0974
1.123 1.0903 1.0998 1.0703 1.1155 1.1248 1.1144 1.1551 1.1877 1.0763
1.0877 1.1382 1.0964 0.9474 1.0007 1.0153 0.9131 1.086 1.1173 1.1058
0.8839 0.8689 0.848 0.7617 0.8664 0.8952 0.7683 0.8349 0.9262 0.9036
0.9905 0.9807 0.9506 0.9723 0.9562 1.0231 1.0179 1.0247 1.0745 0.9572
0.8482 0.917 0.8848 0.8518 0.909 0.8988 0.8404 0.8864 0.9438 0.8568
0.7967 0.9006 0.9231 0.8065 0.8431 0.9068 0.822 0.8772 0.9185 0.8396
0.9317 0.9375 0.9724 0.9772 1.0387 1.0248 0.9109 0.9247 0.9465 0.9122
0.9068 0.9278 0.9566 0.9277 0.9289 0.903 0.9208 0.9389 0.9211 0.92
1.0679 1.103 1.0845 0.9706 0.9617 0.9425 0.9654 1.0559 1.089 1.0331
1.1496 1.1764 1.1612 1.1116 1.0914 1.0557 1.0952 1.2098 1.2686 1.2026
1.1019 1.058 1.0437 1.0304 1.0171 1.0682 1.1201 1.067 1.0919 1.1795
1.2545 1.3273 1.2676 1.2219 1.2573 1.1668 1.1487 1.2985 1.3327 1.2382
1.1748 1.2009 1.1545 1.1078 1.2298 1.2225 1.1695 1.2697 1.2491 1.1392
1.1687 1.1984 1.1368 1.0799 1.0472 1.0091 1.0175 1.1417 1.1907 1.1913
0.9419 1.0009 1.0305 0.9814 0.8663 0.9331 0.9944 1.0862 1.0733 0.9957
0.9515 1.0261 1.0107 1.0145 0.9865 1.0098 1.0245 1.0212 0.995 0.9964
1.0499 1.0701 1.096 1.0642 1.0859 1.0642 1.0355 1.0632 1.1079 1.0617
1.1091 1.1422 1.0486 0.9706 1.0229 1.0072 1.0248 1.1288 1.1331 1.1253
1.0232 1.0682 1.0167 0.9442 1.0085 1.005 0.9991 1.0123 1.0107 1.0031
5;2 6;2 5;1 4;1 3;1 2;1 1;1 2;0 3;0 4;0
0.3505 0.4408 0.3432 0.3361 0.339 0.3554 0.3583 0.3688 0.3579 0.4047
0.4406 0.5391 0.4576 0.4354 0.4385 0.4568 0.4833 0.3869 0.4228 0.4436
0.4519 0.5157 0.4599 0.4082 0.4543 0.3872 0.4019 0.3957 0.4601 0.4446
0.3772 0.4118 0.4101 0.3862 0.3527 0.3474 0.4002 0.3545 0.3492 0.3163
0.3673 0.4359 0.4476 0.4116 0.3958 0.3685 0.4362 0.4014 0.4071 0.4435
0.4079 0.4604 0.3875 0.3655 0.4161 0.3815 0.3597 0.4259 0.441 0.4836
0.5483 0.654 0.5583 0.5567 0.5301 0.5376 0.5586 0.5795 0.5903 0.6136
0.5226 0.5751 0.5332 0.4707 0.4822 0.4546 0.4747 0.4571 0.5004 0.4942
0.4007 0.5194 0.3643 0.3728 0.4668 0.3969 0.4189 0.4186 0.432 0.403
0.4322 0.5243 0.473 0.4437 0.4493 0.4815 0.4688 0.4966 0.4366 0.4579
0.4895 0.5037 0.544 0.5599 0.5206 0.4935 0.4863 0.5209 0.5336 0.5931
0.3713 0.4016 0.4167 0.4207 0.4343 0.4421 0.4416 0.4344 0.4371 0.4336
0.6583 0.6876 0.7153 0.6969 0.6819 0.7206 0.7344 0.773 0.7549 0.7847
0.8636 0.9768 0.8902 0.767 0.7836 0.8157 0.9098 0.8532 0.7851 0.8303
0.6242 0.7292 0.6344 0.5886 0.5882 0.6059 0.6479 0.6711 0.6425 0.6958
0.5765 0.6158 0.5569 0.559 0.6086 0.6049 0.6205 0.6538 0.6189 0.6066
0.9372 0.96 0.9314 0.8672 0.8943 0.8964 0.8701 0.868 0.8738 0.9078
0.575 0.631 0.5563 0.5059 0.4662 0.5406 0.6039 0.5927 0.5246 0.5769
0.9296 1.0988 1.0196 0.9324 0.9165 0.9106 0.9747 1.0438 0.9742 1.0842
0.7865 0.8272 0.7705 0.7377 0.6891 0.7007 0.6833 0.685 0.7091 0.8293
0.7202 0.7542 0.6802 0.656 0.6807 0.6912 0.6892 0.7104 0.7204 0.7398
0.7798 1.1309 0.8856 0.672 0.6592 0.6742 0.7459 0.8851 0.8171 0.922
0.7576 0.8779 0.7821 0.6888 0.7224 0.7111 0.7577 0.7794 0.7604 0.7757
0.731 0.6628 0.7163 0.7082 0.6849 0.6903 0.685 0.7124 0.7261 0.7588
0.9862 0.9995 1.0046 0.9859 0.999 0.9606 0.9609 0.9613 0.9581 1.0231
0.9575 0.9419 0.9664 1.0394 1.068 1.0368 1.048 1.0344 1.0314 1.0385
0.9589 0.979 0.909 0.9641 1.065 1.0504 1.0051 1.0288 1.0123 0.9836
0.9916 1.0537 1.0124 1.0095 0.9875 1.0456 1.021 0.9917 0.9751 1.017
0.9988 1.0409 0.9496 0.992 1.0267 0.9843 0.9405 0.9877 0.9581 1.0439
1.0658 1.2043 0.9906 0.9927 1.0093 1.0245 1.0365 1.1002 1.0692 1.1379
0.9726 1.1241 0.9361 0.9391 0.9928 0.9628 0.9805 0.9473 0.9467 1.0279
0.9833 1.0781 0.9909 0.9421 0.9749 0.9479 0.8811 0.9744 0.9548 1.0487
0.963 1.0953 0.9677 0.9696 1.0308 1.0418 1.0198 1.0691 1.049 1.0877
0.9205 1.0814 1.0098 0.9594 0.9545 0.8934 0.8809 0.9127 0.9821 1.1218
1.0087 1.2134 1.0422 1.0263 1.0666 1.0556 1.0101 1.0893 1.0424 1.0647
0.9818 0.8336 1.0198 1.0364 1.0941 1.0667 1.0242 1.1156 1.084 1.0678
1.0719 1.1098 1.1039 1.1189 1.0766 1.0214 0.9904 1.0389 1.0648 1.0967
0.9947 1.0545 1.0411 1.0271 1.0064 1.0386 1.0284 1.0358 1.016 1.0775
0.9765 0.9964 0.9754 1.0337 1.0414 1.0879 1.1085 1.0518 1.0291 1.0623
0.9795 1.0183 1.0193 0.9886 1.0097 1.0235 0.9745 0.9719 0.8901 0.9921
1.0997 1.1268 1.0824 1.0253 1.0269 0.9339 0.9656 1.0024 1.0382 1.1425
1.0504 1.2148 1.0872 1.0569 1.0632 1.0672 1.0337 1.0417 1.0721 1.1647
1.0149 1.1315 1.0149 0.9127 0.8717 0.9136 0.9659 1.0176 0.9573 1.1097
0.9766 1.0633 1.0394 0.9389 0.9312 0.8644 0.825 0.9065 0.9137 0.9952
0.8649 0.9684 0.9068 0.9102 0.932 0.9132 0.8895 0.9483 0.9216 1.0143
0.9742 1.2044 0.9836 1.0007 1.008 0.9826 1.051 1.0886 1.0571 1.089
0.9328 1.1139 0.9572 0.9711 0.9749 0.917 0.8591 0.9533 0.9764 0.9977
1.0902 0.9427 1.0646 1.0513 1.0326 1.0366 0.9978 1.0504 1.034 1.1042
1.2136 1.1937 1.0981 1.1252 1.0442 1.0754 1.1056 1.0659 1.033 1.0827
1.0125 1.0574 1.0094 0.9514 0.9702 0.9996 1.0029 1.0216 1.0235 0.978
1.1054 1.0873 1.1284 1.1864 1.1743 1.2002 1.2066 1.1703 1.1283 1.1409
1.011 1.0421 0.9691 1.0279 1.0716 1.0552 1.1331 1.1409 1.0666 1.1035
1.0042 1.0413 1.005 0.9525 0.9451 0.9343 0.9086 0.9494 0.9049 0.9569
1.1389 1.2445 1.0831 1.0408 1.0465 1.0533 1.0732 1.0799 1.0815 1.2231
0.9413 1.0675 0.9387 0.8789 0.9557 0.9627 0.9647 0.9952 0.9486 1.0452
0.8769 1.1188 0.9584 0.8406 0.8975 0.8006 0.8454 0.9875 1.0231 1.1341
0.9448 1.0668 0.9697 0.9061 0.8551 0.9249 0.83 0.9669 0.9184 1.0201
0.9703 1.1182 1.1069 0.9985 0.9756 0.9681 0.9449 1.0472 1.0576 1.1126
1.0472 1.1879 1.0552 0.9946 1.0588 1.0388 1.0547 1.0405 1.0626 1.1123
0.9539 0.8322 0.9487 0.9653 1.0231 1.0549 1.0202 1.0494 0.9861 0.9368
1.0497 1.01 1.015 1.1245 1.1639 1.124 1.0628 1.0375 1.1105 1.1374
1.0006 1.0198 1.0435 1.1406 1.1234 1.1298 1.1313 1.1036 1.1689 1.1115
1.0999 1.1129 1.0939 1.0964 1.1823 1.2295 1.233 1.2044 1.1581 1.1061
1.0599 1.1368 1.0524 0.9604 0.971 1.027 0.9765 0.9651 0.9843 1.0322
0.9293 1.0634 0.9375 1.015 1.0513 0.9775 0.9455 1.0529 1.0739 1.1294
1.1616 1.226 1.2023 1.1619 1.2076 1.2182 1.1826 1.1569 1.1292 1.2207
1.0746 1.2616 1.0944 0.9542 1.0195 1.055 0.9636 1.0947 1.0696 1.1798
0.919 1.164 0.9253 0.8589 0.937 0.9137 0.9094 0.9451 0.9792 1.073
0.8408 1.1936 0.8577 0.8211 0.92 0.7749 0.7928 0.8941 0.9477 0.9538
0.9385 1.0791 1.0161 0.9375 0.877 0.8847 0.8621 0.9113 0.8898 1.0987
0.9069 1.0858 0.9277 0.9059 0.9126 0.997 0.9469 1.0427 1.0093 1.0586
1.2606 0.9795 1.1942 1.1641 1.2062 1.1922 1.144 1.1713 1.2092 1.2129
1.0532 1.0891 1.0009 1.0443 1.0912 1.0599 1.0232 1.0885 1.1462 1.0865
0.9749 1.0035 1.0062 0.9465 0.9703 0.9908 1.0183 0.9883 0.9964 1.0868
0.9402 0.9415 0.9099 0.9695 0.948 1.014 1.0211 1.0542 0.9278 0.9048
1.0008 1.077 0.9603 0.9442 1.0395 1.0792 1.1639 1.0415 1.0775 1.0831
1.0107 1.0899 1.0968 1.0688 1.0559 0.9697 1.0334 0.9644 1.0594 1.1059
1.1056 1.2391 1.188 1.059 1.1452 1.0511 1.1189 1.011 1.1012 1.2269
1.1089 1.2774 1.1601 1.1019 1.0824 1.1214 1.0662 1.216 1.0979 1.2027
0.92 1.0228 0.9452 0.8711 0.8565 0.7927 0.8076 0.9136 0.9207 0.9844
1.0006 1.1991 1.0689 0.9464 0.9951 1.0008 1.036 0.9626 0.9675 1.1148
0.8986 1.0841 0.8844 0.8069 0.8324 0.8381 0.8612 0.8739 0.8542 0.9477
0.8582 1.061 1.0235 0.9156 0.8528 0.8418 0.8786 0.9368 0.9326 1.0358
0.8822 0.8311 0.8495 0.8592 0.9096 0.9136 0.8811 0.8859 0.9031 0.8949
0.8927 0.926 0.837 0.8522 0.8385 0.8647 0.8711 0.868 0.8898 0.8521
0.9814 0.9136 0.93 0.962 0.9762 0.9627 0.9571 0.9336 0.931 0.9049
1.1451 1.1516 1.0929 1.1344 1.2178 1.1652 1.1265 1.09 1.1534 1.0794
1.1367 0.949 1.0394 1.0994 1.0414 1.0395 1.0965 1.0358 1.0693 1.0255
1.1759 1.0898 1.0784 1.1395 1.2116 1.2039 1.1098 1.1887 1.1524 1.0583
1.061 0.9593 1.057 1.1036 1.114 1.1638 1.1403 1.0771 1.0544 1.1353
1.1894 1.1032 1.0585 1.0751 1.0691 1.0112 0.982 1.0339 1.0757 1.092
0.9669 0.9943 0.8998 0.9653 1.0277 1.0317 1.0068 1.0043 1.0258 1.0572
0.967 0.906 0.9285 0.9229 0.95 0.9952 0.9895 0.9777 0.9235 0.8769
1.0374 0.9472 0.9954 0.9574 0.9581 1.0186 1.0232 1.0163 0.9523 0.9608
1.1166 1.0327 1.0154 1.1163 1.1535 1.118 1.0992 1.1017 1.0971 1.044
1.0055 0.8647 0.9487 0.9783 0.9676 0.9955 1.0122 1.01 1.0058 0.9941
Blank + 1 2 3 4 5 6 7 8 9
A 0.3845 0.4358 0.425 0.3691 0.3932 0.4126 0.5402 0.4735 0.4375
B 0.7478 0.8666 0.6628 0.6246 0.8931 0.5791 0.9944 0.7875 0.7362
C 0.9805 1.0315 1.0466 1.043 1.0422 1.1027 1.0272 0.9788 1.0365
D 1.0684 1.064 1.0804 1.0244 1.1123 1.103 1.0302 0.9517 0.9152
E 1.1455 1.0465 1.1312 1.0782 1.0674 1.1262 0.9933 0.9722 0.9814
F 1.0856 1.0945 1.1919 1.0428 1.0483 1.1982 1.101 0.984 0.9293
G 1.0579 1.0288 1.0052 1.0599 1.0754 1.126 1.1291 0.9131 1.0208
H 0.9228 1.0206 1.135 1.064 1.1915 1.1654 1.1049 1.0277 0.9559
Blank- 25 12.5 6.25 3.125 1.5625 0.78 0.39 0.195 0.0975
A 0.3037 0.355 0.3442 0.2883 0.3124 0.3318 0.4594 0.3927 0.3567
B 0.667 0.7858 0.582 0.5438 0.8123 0.4983 0.9136 0.7067 0.6554
C 0.8997 0.9507 0.9658 0.9622 0.9614 1.0219 0.9464 0.898 0.9557
D 0.9876 0.9832 0.9996 0.9436 1.0315 1.0222 0.9494 0.8709 0.8344
E 1.0647 0.9657 1.0504 0.9974 0.9866 1.0454 0.9125 0.8914 0.9006
F 1.0048 1.0137 1.1111 0.962 0.9675 1.1174 1.0202 0.9032 0.8485
G 0.9771 0.948 0.9244 0.9791 0.9946 1.0452 1.0483 0.8323 0.94
H 0.842 0.9398 1.0542 0.9832 1.1107 1.0846 1.0241 0.9469 0.8751

0 0.02 0.0487 0.0975 0.195 0.39 0.78 1.5625 3.125

LMB 80 ng 0.3257 0.4243 0.3776 0.3567 0.3927 0.4594 0.3318 0.3124 0.2883
LMB 40 ng 0.6087 0.7064 0.7554 0.6554 0.7067 0.9136 0.4983 0.8123 0.5438
LMB 20 ng 0.9077 0.9752 0.9128 0.9557 0.898 0.9464 1.0219 0.9614 0.9622
LMB 10 ng 0.9396 0.8994 0.9636 0.8344 0.8709 0.9494 1.0222 1.0315 0.9436
LMB 5 ng/ 0.9111 0.9592 0.9545 0.9006 0.8914 0.9125 1.0454 0.9866 0.9974
LMB 2.5 n 1.1144 0.8838 0.8845 0.8485 0.9032 1.0202 1.1174 0.9675 0.962
LMB 1.25 0.8293 0.8129 0.8139 0.94 0.8323 1.0483 1.0452 0.9946 0.9791
LMB 0 ng/ 0.9121 1.0221 0.9739 0.8751 0.9469 1.0241 1.0846 1.1107 0.9832

ZANAMIVIR-LMB CYTOTOXICITY ACTIVITY ON MDCK CELLS

1.2
Absorbance (A560nm)

0.8

0.6

0.4

0.2

0
0 5 10 15 20 25

Well Mean Zanamivir conc (uM)

A1 0.3845
 0 5 10 15 20 25

Zanamivir conc (uM)

A2 0.4358
A3 0.425 ZANAMIVIR-LMB CYTOTOXICITY ACTIVITY ON MDCK CELLS
A4 0.3691 1.2

Absorbance (A560nm)
A5 0.3932
A6 0.4126 1
A7 0.5402 0.8
A8 0.4735
A9 0.4375 0.6
A10 0.4584
0.4
A11 0.5051
A12 0.4065 0.2
B1 0.7478
B2 0.8666 0
0 0.5 1 1.5 2 2.5
B3 0.6628
B4 0.6246 Zanamivir conc (uM)
B5 0.8931
B6 0.5791 ZANAMIVIR-LMB CYTOTOXICITY ACTIVITY ON MDCK CELLS
B7 0.9944 1.2
B8 0.7875
Absorbance (A560nm)

B9 0.7362 1
B10 0.8362
0.8
B11 0.7872
B12 0.6895 0.6
C1 0.9805
C2 1.0315 0.4
C3 1.0466 0.2
C4 1.043
C5 1.0422 0
C6 1.1027 0 0.02 0.0487 0.0975 0.195 0.39 0.78 1.5625 3.125 6.25 12.
C7 1.0272 Zanamivir conc (uM)
C8 0.9788
C9 1.0365
C10 0.9936 ZANAMIVIR-LMB CYTOTOXICITY ACTIVITY ON MDCK CELLS
C11 1.056
1.2
C12 0.9885
D1 1.0684 1
D2 1.064
D3 1.0804 0.8
D4 1.0244
0.6
D5 1.1123
D6 1.103 0.4
D7 1.0302
D8 0.9517 0.2
D9 0.9152
0
D10 1.0444 0 1 2 3 4 5 6 7 8 9
D11 0.9802
D12 1.0204
E1 1.1455
E2 1.0465
E3 1.1312
E4 1.0782
E5 1.0674
E6 1.1262
E7 0.9933
E8 0.9722
E9 0.9814
E10 1.0353
E11 1.04
E12 0.9919
F1 1.0856
F2 1.0945
F3 1.1919
F4 1.0428
F5 1.0483
F6 1.1982
F7 1.101
F8 0.984
F9 0.9293
F10 0.9653
F11 0.9646
F12 1.1952
G1 1.0579
G2 1.0288
G3 1.0052
G4 1.0599
G5 1.0754
G6 1.126
G7 1.1291
G8 0.9131
G9 1.0208
G10 0.8947
G11 0.8937
G12 0.9101
H1 0.9228
H2 1.0206
H3 1.135
H4 1.064
H5 1.1915
H6 1.1654
H7 1.1049
H8 1.0277
H9 0.9559
H10 1.0547
H11 1.1029
H12 0.9929
End Time: 2011/02/12 14:12:52

Movement Move Plate Out

 10 11 12
0.4584 0.5051 0.4065
0.8362 0.7872 0.6895
0.9936 1.056 0.9885
1.0444 0.9802 1.0204
1.0353 1.04 0.9919
0.9653 0.9646 1.1952
0.8947 0.8937 0.9101
1.0547 1.1029 0.9929
0.0487 0.02 0
0.3776 0.4243 0.3257
0.7554 0.7064 0.6087
0.9128 0.9752 0.9077
0.9636 0.8994 0.9396
0.9545 0.9592 0.9111
0.8845 0.8838 1.1144
0.8139 0.8129 0.8293
0.9739 1.0221 0.9121

6.25 12.5 25
0.3442 0.355 0.3037
0.582 0.7858 0.667
0.9658 0.9507 0.8997
0.9996 0.9832 0.9876
1.0504 0.9657 1.0647
1.1111 1.0137 1.0048
0.9244 0.948 0.9771
1.0542 0.9398 0.842

N MDCK CELLS

LMB 80 ng/ml
LMB 40 ng/ml
LMB 5 ng/ml
LMB 2.5 ng/ml
LMB 1.25 ng/ml
LMB 0 ng/ml

20 25 30
 20 25 30

N MDCK CELLS

LMB 80 ng/ml
LMB 20 ng/ml
LMB 5 ng/ml
LMB 2.5 ng/ml
LMB 0 ng/ml

2 2.5 3

TIVITY ON MDCK CELLS

LMB 80 ng/ml
LMB 40 ng/ml
LMB 20 ng/ml
LMB 10 ng/ml
LMB 5 ng/ml
LMB 2.5 ng/ml
LMB 1.25 ng/ml
LMB 0 ng/ml

1.5625 3.125 6.25 12.5 25

ON MDCK CELLS

LMB 80 ng/ml
LMB 40 ng/ml
LMB 10 ng/ml
LMB 0 ng/ml

7 8 9 10
 1.2

0.8

Absorbance
0.6

(560 nm)
0.4

0.2

0
25 .5 25
12 6. 3
 1.2

0.8
LMB-80
Absorbance

0.6 LMB-40
LMB-20
(560 nm)

0.4 LMB-10
LMB-5
0.2
LMB-2.25
0 LMB-1.25
LMB-0.625
25 .5 25 25 25 .78 .39 195 975 487 .02 0
12 6. 3.1 .56 0 0 0. 0 0 0
1 0. 0.
Zanamir conc (μM)