1 2 3 4 5 6 7 8

A 0.8273 0.7863 0.6786 0.6681 0.5326 0.5846 0.481 0.3402

B 0.886 0.9764 0.9263 0.8676 0.7931 0.9061 0.7643 0.5876
C 1.0411 0.9791 0.965 0.9276 0.9241 0.7761 0.7855 0.6851
D 1.2587 1.0113 0.9862 0.8367 1.0864 0.9244 0.9923 0.9459
E 1.0968 0.9953 1.0027 0.9985 0.9722 1.0611 1.0437 0.7536
F 1.007 0.9913 1.0034 1.0469 1.1284 1.0449 0.973 0.8748
G 0.547 0.5308 0.6068 0.8354 0.7097 0.6877 0.9905 0.5488
H 0.3856 0.3604 0.4012 0.3841 0.4872 0.4196 0.4018 0.4115
BLANK - 25 12.5 6.25 3.125 1.5625 0.78 0.39 0.195
A 0.7465 0.7055 0.5978 0.5873 0.4518 0.5038 0.4002 0.2594
B 0.8052 0.8956 0.8455 0.7868 0.7123 0.8253 0.6835 0.5068
C 0.9603 0.8983 0.8842 0.8468 0.8433 0.6953 0.7047 0.6043
D 1.1779 0.9305 0.9054 0.7559 1.0056 0.8436 0.9115 0.8651
E 1.016 0.9145 0.9219 0.9177 0.8914 0.9803 0.9629 0.6728
F 0.9262 0.9105 0.9226 0.9661 1.0476 0.9641 0.8922 0.794
G 0.4662 0.45 0.526 0.7546 0.6289 0.6069 0.9097 0.468
H 0.3048 0.2796 0.3204 0.3033 0.4064 0.3388 0.321 0.3307
Well Mean
A1 0.8273
A2 0.7863
A3 0.6786
A4 0.6681
A5 0.5326
A6 0.5846
A7 0.481
A8 0.3402
A9 0.2912
A10 0.4394
A11 0.1002
A12 0.0954
B1 0.886
B2 0.9764
B3 0.9263
B4 0.8676
B5 0.7931
B6 0.9061
B7 0.7643
B8 0.5876
B9 0.4144
B10 0.8691
B11 0.1136
B12 0.1124
C1 1.0411
C2 0.9791
C3 0.965
C4 0.9276
C5 0.9241
C6 0.7761
C7 0.7855
C8 0.6851
C9 0.6855
C10 0.1907
C11 0.2033
C12 0.1182
D1 1.2587
D2 1.0113
D3 0.9862
D4 0.8367
D5 1.0864
D6 0.9244
D7 0.9923
D8 0.9459
D9 0.944
D10 0.782
D11 0.2941
D12 0.1189
E1 1.0968
E2 0.9953
E3 1.0027
E4 0.9985
E5 0.9722
E6 1.0611
E7 1.0437
E8 0.7536
E9 0.9145
E10 0.2129
E11 0.1218
E12 0.1068
F1 1.007
F2 0.9913
F3 1.0034
F4 1.0469
F5 1.1284
F6 1.0449
F7 0.973
F8 0.8748
F9 0.7827
F10 0.6263
F11 0.6016
F12 0.1331
G1 0.547
G2 0.5308
G3 0.6068
G4 0.8354
G5 0.7097
G6 0.6877
G7 0.9905
G8 0.5488
G9 0.4983
G10 0.4653
G11 0.3049
G12 0.3427
H1 0.3856
H2 0.3604
H3 0.4012
H4 0.3841
H5 0.4872
H6 0.4196
H7 0.4018
H8 0.4115
H9 0.4005
H10 0.366
H11 0.3254
H12 0.2898

End Time: 2011/02/12 14:27:39

Movement Move Plate Out

 9 10 11 12
0.2912 0.4394 0.1002 0.0954
0.4144 0.8691 0.1136 0.1124
0.6855 0.1907 0.2033 0.1182
0.944 0.782 0.2941 0.1189
0.9145 0.2129 0.1218 0.1068
0.7827 0.6263 0.6016 0.1331
0.4983 0.4653 0.3049 0.3427
0.4005 0.366 0.3254 0.2898
0.0975 0.0487 0.02 0
0.2104 0.3586 0.0194 0.0146
0.3336 0.7883 0.0328 0.0316
0.6047 0.1099 0.1225 0.0374
1.4
0.8632 0.7012 0.2133 0.0381
0.8337 0.1321 0.041 0.026 1.2
0.7019 0.5455 0.5208 0.0523
0.4175 0.3845 0.2241 0.2619 1

Absorbance (560
0.3197 0.2852 0.2446 0.209 0.8

0.6

0.4

Nm)
0.2

0
25
12
 1.4

1.2

1
LMB-80
Absorbance (560

0.8 LMB-40
LMB-20
0.6 LMB-10
LMB-5
0.4 LMB-2.5
Nm)

LMB-1.25
0.2 LMB-0.00

0
25 .5 25 1 2 5 6 2 5 78 39 195 97 5 4 87 02 0
12 6. 3. 5 0. 0. 0. 0 0 0.
1. 0. 0.
Zanamivir Conc (μM)
Application: Tecan i-control Tecan i-control , 1.5.14.0
Device: infinite 200 Serial number: 901006981 Serial number of connected stacker:
Firmware: V_2.11_04/08_InfiniTe (Apr 4 2 MAI, V_2.11_04/08_InfiniTe (Apr 4 2008/14.37.11)

Date: ###
Time: 14:16:53

System FM-144598422
User FM-144598422\Administrator
Plate Nunclon 96 Flat Bottom Transparent Polystyrol [NUN96ft.pdfx]
Plate-ID (Stacker)

Label: Label1
Mode Absorbance
Multiple Reads per Well (Circle (filled)) 7x7
Multiple Reads per Well (Border) 200 µm
Wavelength 560 nm
Bandwidth 9 nm
Number of Flashes 5
Settle Time 0 ms
Start Time:2011/02/12 14:16:53

Multiple Reads Per Well -

Alignment

111

11111

1111111

1111111

1111111

11111

111 1
Well Mean StDev 2;6 3;6 4;6 5;5 4;5 3;5 2;5
A1 0.8273 0.0463 0.7303 0.7152 0.7341 0.8198 0.8644 0.7684 0.8105
A2 0.7863 0.056 0.8449 0.81 0.7984 0.8081 0.7931 0.7486 0.7912
A3 0.6786 0.1027 0.699 0.6598 0.6735 0.6538 0.6132 0.5684 0.6021
A4 0.6681 0.187 0.8558 0.7811 0.691 0.4955 0.5246 0.6485 0.7871
A5 0.5326 0.0734 0.6521 0.5939 0.5829 0.5654 0.513 0.4924 0.5663
A6 0.5846 0.1116 0.8532 0.7041 0.6788 0.5318 0.5478 0.5719 0.6986
A7 0.481 0.134 0.7068 0.7367 0.713 0.6577 0.6079 0.5066 0.5449
A8 0.3402 0.1326 0.5557 0.4783 0.4452 0.343 0.3293 0.3573 0.4535
A9 0.2912 0.0503 0.3485 0.341 0.3541 0.3142 0.2698 0.2476 0.2905
A10 0.4394 0.1172 0.3062 0.4035 0.5412 0.6286 0.5023 0.3622 0.2634
A11 0.1002 0.0461 0.17 0.1533 0.1679 0.1463 0.097 0.0766 0.1014
A12 0.0954 0.044 0.182 0.1543 0.1535 0.1362 0.1009 0.0836 0.1109
B1 0.886 0.0604 0.8955 0.9909 0.9715 0.8824 0.8391 0.8324 0.9478
B2 0.9764 0.0609 0.949 1.0529 1.1082 1.1164 1.0129 0.9695 0.9121
B3 0.9263 0.052 0.9464 1.0619 0.9761 0.9217 0.8901 0.8705 0.8698
B4 0.8676 0.162 1.25 1.2477 1.0583 0.9693 0.9393 0.9254 1.0024
B5 0.7931 0.1799 1.0973 1.1635 1.038 0.9938 0.8683 0.7648 0.8661
B6 0.9061 0.1855 1.3009 1.278 1.1047 0.8906 0.9012 0.9352 1.0804
B7 0.7643 0.1757 1.0771 1.0885 1.0207 0.7939 0.8189 0.8209 0.9326
B8 0.5876 0.1712 0.9829 0.9876 0.8634 0.7285 0.7112 0.6532 0.7274
B9 0.4144 0.161 0.6994 0.6664 0.5822 0.6117 0.4891 0.401 0.4613
B10 0.8691 0.0554 0.9862 0.9793 0.9187 0.9435 0.8344 0.803 0.834
B11 0.1136 0.06 0.2279 0.2321 0.2575 0.194 0.1313 0.0949 0.1195
B12 0.1124 0.0585 0.2396 0.2174 0.1793 0.1288 0.1168 0.099 0.14
C1 1.0411 0.0696 0.9581 1.0475 1.0251 1.1544 0.99 0.9647 0.9905
C2 0.9791 0.0759 1.0239 1.1022 1.1374 0.9885 1.0158 0.9553 0.9431
C3 0.965 0.0964 1.1143 1.2345 1.1221 1.0616 1.0563 1.0039 0.9813
C4 0.9276 0.1367 1.1346 1.1474 1.1051 1.1164 1.0292 0.9441 0.998
C5 0.9241 0.186 1.2072 1.3071 1.311 1.2569 1.0919 1.0024 0.9414
C6 0.7761 0.1912 1.188 1.1666 1.0823 0.8748 0.8425 0.7529 0.8871
C7 0.7855 0.1451 1.0288 1.0224 0.9816 0.9207 0.7833 0.7181 0.7816
C8 0.6851 0.2208 1.0981 1.1229 0.9959 0.8065 0.7746 0.7297 0.8571
C9 0.6855 0.1356 0.9117 0.9379 0.8949 0.7606 0.7191 0.7348 0.8283
C10 0.1907 0.1054 0.4086 0.406 0.4016 0.2787 0.2379 0.1857 0.2459
C11 0.2033 0.0432 0.2456 0.2508 0.2651 0.2279 0.1809 0.1531 0.1659
C12 0.1182 0.0637 0.2744 0.247 0.2001 0.1366 0.1203 0.111 0.1491
D1 1.2587 0.0601 1.1563 1.2491 1.2263 1.2904 1.236 1.2065 1.2256
D2 1.0113 0.0917 1.1499 1.225 1.1677 1.0205 1.0269 0.956 1.0003
D3 0.9862 0.1441 1.2964 1.2674 1.2226 1.1596 1.0934 1.0345 1.0298
D4 0.8367 0.1905 1.2659 1.1887 1.0656 0.9546 0.8924 0.8216 0.9786
D5 1.0864 0.1434 1.323 1.2889 1.2182 1.2314 1.0992 0.9969 0.9735
D6 0.9244 0.2394 1.36 1.296 1.0739 0.8893 0.8765 0.9163 1.1076
D7 0.9923 0.1727 1.3499 1.2951 1.1856 1.155 1.1257 1.0192 1.0558
D8 0.9459 0.0848 1.0273 1.1066 1.0757 1.0508 0.9867 0.8984 0.9333
D9 0.944 0.1024 1.1058 1.1135 1.0708 1.0268 1.031 0.8911 0.9867
D10 0.782 0.0686 0.7935 0.8269 0.825 0.7879 0.7614 0.7593 0.7604
D11 0.2941 0.1267 0.2804 0.2837 0.2881 0.2648 0.1903 0.142 0.1697
D12 0.1189 0.0653 0.2773 0.2517 0.2234 0.1671 0.1463 0.1201 0.1641
E1 1.0968 0.0767 1.0593 1.2555 1.1625 1.3055 1.2221 1.0953 1.1001
E2 0.9953 0.1089 1.1268 1.2267 1.1792 1.0988 1.1371 1.0704 1.0171
E3 1.0027 0.1056 1.1125 1.2427 1.2684 1.0842 1.0701 1.068 1.0155
E4 0.9985 0.0973 1.1427 1.1757 1.1149 1.0617 1.0199 0.9161 0.9812
E5 0.9722 0.1495 1.2506 1.2361 1.1929 1.1176 1.1052 0.9966 1.0559
E6 1.0611 0.0933 1.1594 1.247 1.1917 1.0399 1.0843 0.9955 1.111
E7 1.0437 0.1156 1.2069 1.2227 1.1655 1.055 1.0482 1.0222 1.0541
E8 0.7536 0.2586 1.0322 1.0254 1.0511 1.1674 0.9215 0.7145 0.7182
E9 0.9145 0.072 0.9794 1.0078 1.0369 0.9505 0.9619 0.81 0.8774
E10 0.2129 0.143 0.5267 0.4966 0.4565 0.3555 0.3083 0.2315 0.2878
E11 0.1218 0.0643 0.2596 0.2561 0.2535 0.2009 0.1467 0.113 0.141
E12 0.1068 0.054 0.2601 0.2294 0.1917 0.1279 0.1183 0.11 0.1439
F1 1.007 0.1058 1.0493 1.2881 1.2102 1.0956 1.1008 1.1107 1.0942
F2 0.9913 0.0749 1.1066 1.1363 1.1693 1.067 1.0861 0.9976 1.0014
F3 1.0034 0.123 1.2059 1.1624 1.1764 1.1219 1.0239 0.9457 1.0502
F4 1.0469 0.0957 1.1865 1.2555 1.1313 1.1327 1.1347 0.9897 1.0166
F5 1.1284 0.0879 1.2892 1.3065 1.2512 1.1423 1.148 1.0711 1.1132
F6 1.0449 0.1014 1.1975 1.1896 1.1713 1.1657 1.0871 0.9393 1.0095
F7 0.973 0.0933 0.9137 1.0675 1.067 1.0403 1.0151 0.9152 0.7901
F8 0.8748 0.0982 1.0158 1.0681 1.0294 0.7787 0.9021 0.8759 0.8977
F9 0.7827 0.1016 0.9433 1.0146 0.9234 0.9164 0.8089 0.7521 0.8119
F10 0.6263 0.1235 0.7964 0.9554 0.8361 0.7477 0.7322 0.6765 0.688
F11 0.6016 0.0655 0.581 0.5412 0.5141 0.5752 0.4746 0.5152 0.4903
F12 0.1331 0.0745 0.3305 0.3048 0.2552 0.1591 0.1571 0.1416 0.1799
G1 0.547 0.0694 0.5865 0.6682 0.7057 0.6969 0.6159 0.5556 0.543
G2 0.5308 0.0623 0.65 0.6921 0.6856 0.6304 0.5678 0.5657 0.5442
G3 0.6068 0.0973 0.7976 0.8228 0.8081 0.7393 0.6923 0.6329 0.6578
G4 0.8354 0.105 1.0225 1.0953 0.9829 0.9214 0.9139 0.7985 0.8571
G5 0.7097 0.1338 0.9992 0.996 0.8852 0.8561 0.8032 0.7428 0.7958
G6 0.6877 0.1783 1.0576 1.0185 0.9765 0.8757 0.801 0.6362 0.7128
G7 0.9905 0.1826 1.1006 1.2362 1.2764 1.1629 1.1745 0.9661 0.8942
G8 0.5488 0.1861 0.8903 0.7821 0.7195 0.6956 0.5939 0.5195 0.5873
G9 0.4983 0.1362 0.8185 0.7138 0.6936 0.6372 0.589 0.4664 0.5523
G10 0.4653 0.1265 0.7101 0.6914 0.6601 0.5729 0.5058 0.4214 0.499
G11 0.3049 0.0506 0.3994 0.407 0.4196 0.3767 0.3194 0.3026 0.315
G12 0.3427 0.0587 0.487 0.48 0.3794 0.3235 0.3348 0.3475 0.3962
H1 0.3856 0.051 0.4591 0.4867 0.489 0.4717 0.422 0.3559 0.396
H2 0.3604 0.0509 0.4848 0.4726 0.477 0.3905 0.3935 0.3511 0.3865
H3 0.4012 0.064 0.5067 0.5635 0.5821 0.5254 0.4495 0.3799 0.3582
H4 0.3841 0.0465 0.5035 0.5001 0.4636 0.4253 0.3522 0.3772 0.3865
H5 0.4872 0.0924 0.6444 0.6866 0.7131 0.6188 0.5845 0.4516 0.4971
H6 0.4196 0.051 0.4785 0.5186 0.5052 0.4867 0.4187 0.3487 0.3792
H7 0.4018 0.0854 0.5836 0.5966 0.6187 0.501 0.5066 0.4138 0.4293
H8 0.4115 0.0786 0.5608 0.5816 0.562 0.5011 0.4846 0.3931 0.4407
H9 0.4005 0.0676 0.5765 0.552 0.5375 0.4782 0.4189 0.3885 0.412
H10 0.366 0.0463 0.4781 0.4575 0.4533 0.4288 0.3695 0.3514 0.3807
H11 0.3254 0.0455 0.4384 0.4094 0.4018 0.3971 0.3477 0.3261 0.3181
H12 0.2898 0.0432 0.408 0.3922 0.3499 0.306 0.2978 0.2763 0.2881

End Time: 2011/02/12 14:27:39

Movement Move Plate Out

mber of connected stacker:
1;5 0;4 1;4 2;4 3;4 4;4 5;4 6;4 6;3 5;3
0.9104 0.8719 0.841 0.802 0.7683 0.7995 0.8401 0.8449 0.859 0.8248
0.8733 0.8217 0.7874 0.7449 0.7041 0.706 0.7588 0.7265 0.7999 0.6846
0.7478 0.8286 0.6898 0.5762 0.5699 0.5969 0.6407 0.7958 0.7361 0.6297
0.8952 0.9546 0.8153 0.7394 0.6194 0.4526 0.3447 0.297 0.3486 0.3811
0.6537 0.7139 0.5524 0.4985 0.4665 0.4565 0.5008 0.5363 0.487 0.4658
0.7263 0.7458 0.541 0.4737 0.4341 0.4572 0.5268 0.6644 0.7152 0.5673
0.5359 0.5899 0.3572 0.3687 0.3616 0.4269 0.5803 0.6521 0.6452 0.5119
0.5778 0.5747 0.4748 0.3983 0.3705 0.3263 0.2949 0.3273 0.2516 0.1993
0.3839 0.3902 0.287 0.2685 0.2908 0.2829 0.269 0.279 0.2785 0.2822
0.2766 0.3741 0.2553 0.2585 0.3999 0.5019 0.5378 0.5803 0.6062 0.521
0.1481 0.212 0.0934 0.059 0.0591 0.0602 0.0697 0.1158 0.1134 0.0628
0.1611 0.2116 0.0946 0.058 0.0571 0.0577 0.0652 0.1074 0.0987 0.0582
0.9333 0.9094 0.9127 0.8626 0.8367 0.73 0.8392 0.9571 0.8758 0.7593
0.9508 1.0372 0.9416 0.9877 1.0021 1.0157 0.9935 1.0246 1.004 0.9717
0.9262 1.0061 0.9113 0.8752 0.8812 0.8881 0.8938 1.0127 0.9074 0.8977
1.1537 1.1565 0.7857 0.6795 0.6704 0.7102 0.8254 0.958 0.9208 0.7671
1.1039 1.1797 0.7675 0.5919 0.6032 0.6603 0.7946 1.0086 0.9227 0.6744
1.2223 1.1613 0.901 0.8267 0.7718 0.6934 0.7069 0.8241 0.6859 0.62
1.056 1.1203 0.8753 0.717 0.6882 0.5975 0.6346 0.7639 0.6434 0.5112
0.8604 0.9001 0.6155 0.4863 0.4976 0.4616 0.5289 0.5302 0.4553 0.4157
0.5916 0.6195 0.2804 0.2329 0.2673 0.3249 0.5093 0.7516 0.6591 0.4405
0.9101 0.9339 0.8419 0.8082 0.8045 0.8442 0.89 0.9673 0.9303 0.8313
0.1832 0.2013 0.0818 0.059 0.06 0.0612 0.0882 0.1742 0.1518 0.0691
0.2146 0.2535 0.1084 0.0626 0.0617 0.0615 0.0808 0.1066 0.1234 0.0628
0.945 0.9457 0.9969 1.0552 1.034 1.0455 1.0523 1.0884 0.9296 1.0261
1.0727 1.0828 0.9255 0.8737 0.9376 0.9566 0.9947 1.0911 1.0273 0.9707
1.0086 1.0487 0.8395 0.8053 0.9394 0.8963 0.9847 1.0904 1.0418 0.8974
1.1251 1.1448 0.9507 0.8195 0.8735 0.8768 1.0068 1.0713 0.9536 0.9109
1.0279 1.0884 0.7188 0.7425 0.838 0.9092 1.0715 1.2033 1.1306 0.8897
1.1266 1.2503 0.7944 0.5932 0.5798 0.5872 0.7093 0.8144 0.7586 0.5998
0.9601 1.1006 0.7326 0.5826 0.5922 0.6 0.7925 0.9313 0.9009 0.7017
1.088 1.2548 0.7927 0.5856 0.5368 0.5202 0.6124 0.57 0.4557 0.4198
0.891 0.958 0.7292 0.628 0.5575 0.5287 0.6326 0.7822 0.6285 0.5556
0.3343 0.3968 0.1569 0.0856 0.0817 0.0768 0.1655 0.2889 0.2166 0.096
0.2241 0.2281 0.1549 0.1787 0.2201 0.2145 0.2125 0.2987 0.2707 0.2358
0.2209 0.2582 0.1036 0.0623 0.0638 0.0621 0.0816 0.128 0.1345 0.0614
1.1661 1.1302 1.2105 1.2462 1.2824 1.3178 1.2726 1.3127 1.2498 1.2776
1.0713 1.1252 0.8757 0.8853 0.9206 0.9299 1.0344 1.163 1.0794 0.9725
1.2089 1.2165 0.8553 0.7676 0.8603 0.9264 1.0619 1.062 1.0083 0.9248
1.1707 1.2805 0.8511 0.6509 0.6138 0.6539 0.821 1.006 0.9711 0.7597
1.2675 1.3666 0.9463 0.7817 0.8699 0.9464 1.1728 1.3006 1.21 1.012
1.3277 1.4361 1.0549 0.8555 0.6954 0.605 0.7334 0.7895 0.6707 0.5848
1.247 1.3489 0.9742 0.8317 0.8466 0.8965 1.0258 1.2414 1.1063 0.9453
1.0032 1.0986 0.8747 0.7865 0.8228 0.8389 0.9478 1.0775 1.0544 0.888
1.0575 1.1897 0.8714 0.7929 0.8568 0.9004 0.9716 1.0562 1.0156 0.8659
0.8558 0.8594 0.7974 0.7814 0.7671 0.7741 0.7096 0.7089 0.624 0.6662
0.2425 0.2455 0.1295 0.1176 0.1198 0.1476 0.235 0.3728 0.4542 0.2919
0.2396 0.243 0.0982 0.0617 0.0628 0.0637 0.1006 0.1503 0.1314 0.0637
1.0325 1.0242 1.0184 1.1033 1.1931 1.2049 1.1631 1.122 1.0656 1.083
1.126 1.2679 0.9979 0.9132 0.9859 0.9392 1.0338 1.0545 0.9092 0.8628
1.0756 1.2431 0.9585 0.8945 0.9241 0.907 0.9748 1.1322 1.0405 0.9289
1.1003 1.2634 1.0046 0.9183 0.8771 0.8656 0.9517 1.051 0.9956 0.9024
1.2282 1.3009 0.987 0.9051 0.8948 0.8718 1.0003 1.0512 1.0262 0.8913
1.1418 1.2179 1.0059 0.9204 0.9199 0.9451 0.977 0.9982 0.9339 0.8888
1.1706 1.2996 1.0018 0.9211 0.8874 0.8589 0.9465 1.0238 0.9365 0.9156
0.799 0.7951 0.4305 0.4526 0.5477 0.7223 1.0267 1.2622 1.2219 0.8966
0.9949 0.9963 0.8025 0.7835 0.7953 0.8062 0.8953 0.9661 0.9668 0.8694
0.4009 0.4834 0.1634 0.0803 0.0856 0.0899 0.2276 0.366 0.3008 0.1258
0.2235 0.2264 0.0889 0.0629 0.0624 0.0689 0.1175 0.2014 0.1428 0.0745
0.2136 0.1997 0.0842 0.0596 0.0604 0.0598 0.0888 0.1232 0.1103 0.0627
1.0723 0.9805 0.9745 0.9969 1.0047 0.9339 1.0199 1.1396 1.0754 0.9743
1.0648 1.0614 0.9253 0.9179 0.9058 0.9192 1.0064 1.1121 1.0404 0.9545
1.1565 1.2359 1.0054 0.9262 0.8969 0.841 0.9611 1.1114 1.1374 0.8968
1.1245 1.1906 0.9199 0.8797 0.9351 1.0159 1.1168 1.2167 1.1346 1.0082
1.2172 1.3228 1.087 1.0225 1.0321 1.0035 1.1268 1.2929 1.182 1.0349
1.2157 1.2577 0.9456 0.8372 0.859 0.9578 1.1032 1.1098 1.0634 0.9524
0.7795 0.9439 0.7316 0.7841 0.9313 0.9153 0.9935 1.0128 0.9781 0.9214
0.9652 1.0514 0.7754 0.7224 0.7686 0.7195 0.7624 0.7665 0.9329 0.8071
0.9076 1.0028 0.7667 0.6877 0.6506 0.6326 0.7595 0.8019 0.7794 0.6327
0.7407 0.7826 0.5814 0.5585 0.5275 0.5459 0.6734 0.8177 0.7435 0.5821
0.5819 0.5965 0.475 0.468 0.5513 0.5562 0.6101 0.6479 0.6304 0.596
0.2762 0.2732 0.1037 0.067 0.0658 0.0713 0.1223 0.158 0.1001 0.073
0.5504 0.5753 0.5536 0.534 0.5067 0.5388 0.5762 0.7317 0.6315 0.518
0.5831 0.5828 0.5188 0.5119 0.5032 0.4925 0.5422 0.6296 0.5767 0.4964
0.7212 0.7421 0.5623 0.5254 0.5312 0.5184 0.6306 0.7484 0.6581 0.5353
0.888 1.0296 0.7343 0.6906 0.7011 0.7307 0.8704 0.9391 0.9375 0.8352
0.8919 0.9485 0.63 0.5634 0.5844 0.5888 0.7599 0.8683 0.7819 0.6199
0.917 1.0377 0.6175 0.498 0.4915 0.5477 0.6647 0.9349 0.8542 0.5827
0.8413 1.0645 0.754 0.7323 0.8103 0.8793 1.0887 1.3497 1.3483 1.0446
0.7633 0.873 0.4642 0.357 0.3531 0.4372 0.5793 0.8755 0.8458 0.4921
0.6351 0.7192 0.4281 0.3784 0.3518 0.3948 0.5728 0.7199 0.6496 0.4028
0.6159 0.69 0.4041 0.3585 0.3413 0.3417 0.5009 0.6546 0.601 0.4326
0.3873 0.3827 0.2949 0.2716 0.2561 0.2397 0.279 0.3518 0.3453 0.2532
0.4582 0.4284 0.3412 0.3289 0.3038 0.2699 0.3005 0.3017 0.2597 0.2735
0.4238 0.3923 0.3569 0.3714 0.3321 0.355 0.4015 0.4947 0.4385 0.3485
0.4371 0.4284 0.3357 0.3642 0.363 0.3131 0.3623 0.4457 0.3544 0.2987
0.4364 0.4631 0.3548 0.3532 0.3699 0.3651 0.4151 0.4782 0.416 0.3206
0.4521 0.4142 0.3423 0.3746 0.3555 0.347 0.3666 0.4476 0.405 0.2885
0.6009 0.6052 0.4344 0.4148 0.399 0.3946 0.5271 0.6529 0.5304 0.4102
0.4638 0.472 0.3899 0.4175 0.3616 0.3633 0.4244 0.534 0.4916 0.4101
0.5076 0.4777 0.3291 0.3316 0.3457 0.3292 0.419 0.4721 0.4588 0.3303
0.4646 0.4348 0.3019 0.353 0.3481 0.2979 0.4419 0.5358 0.5306 0.3669
0.5042 0.5018 0.374 0.415 0.3723 0.3135 0.3912 0.4698 0.4336 0.3275
0.4217 0.4214 0.3466 0.326 0.3206 0.3134 0.361 0.4623 0.3808 0.3199
0.401 0.3333 0.3198 0.297 0.323 0.3035 0.3297 0.4083 0.324 0.3027
0.3554 0.3841 0.2776 0.2313 0.2338 0.2365 0.3006 0.3094 0.2693 0.2466
4;3 3;3 2;3 1;3 0;3 0;2 1;2 2;2 3;2 4;2
0.8374 0.8594 0.8224 0.7826 0.8514 0.8668 0.7893 0.8489 0.8733 0.848
0.7058 0.7517 0.7717 0.7787 0.8565 0.8297 0.7844 0.8088 0.8376 0.7506
0.6284 0.6236 0.6094 0.6923 0.8765 0.8882 0.7561 0.6962 0.6748 0.6159
0.5993 0.6771 0.7608 0.79 0.9578 0.9871 0.838 0.8021 0.7084 0.6303
0.4784 0.4857 0.5199 0.5185 0.6868 0.7074 0.5413 0.5088 0.5027 0.5227
0.5054 0.4517 0.4167 0.4398 0.6632 0.7076 0.4711 0.4542 0.5052 0.5735
0.4338 0.3785 0.3501 0.2675 0.4936 0.4771 0.2509 0.3153 0.3563 0.4156
0.255 0.3075 0.362 0.4174 0.5497 0.5384 0.4105 0.3267 0.2493 0.1741
0.3398 0.3643 0.3256 0.2744 0.3227 0.2994 0.2269 0.274 0.3517 0.3631
0.5282 0.4562 0.3137 0.2613 0.4134 0.4675 0.2855 0.2821 0.4371 0.5431
0.0645 0.0642 0.0633 0.0698 0.2045 0.2064 0.0743 0.065 0.0674 0.0635
0.0588 0.0613 0.0611 0.0651 0.1788 0.1741 0.0664 0.0611 0.0623 0.0591
0.8018 0.9223 0.9244 0.8963 0.9457 0.8917 0.9259 0.9877 0.9313 0.8106
0.979 1.0325 0.9927 0.937 0.9302 0.8517 0.8608 0.9893 1.0423 1.0051
0.9353 0.9446 0.9222 0.8985 1.0029 0.9695 0.9035 0.9554 0.9727 0.9469
0.757 0.7201 0.6484 0.658 0.973 0.922 0.6468 0.7208 0.8185 0.8258
0.6875 0.6467 0.5551 0.6239 0.9496 0.9083 0.6055 0.5905 0.6616 0.6394
0.7025 0.8476 0.8478 0.8501 1.0842 1.1859 0.883 0.963 0.951 0.75
0.5884 0.7244 0.7246 0.7596 1.0343 0.9503 0.6848 0.6889 0.7375 0.5842
0.4899 0.5112 0.4808 0.5199 0.8478 0.7587 0.4523 0.4559 0.5076 0.4465
0.3968 0.3511 0.2315 0.1964 0.4809 0.4407 0.1656 0.2229 0.3493 0.3653
0.9133 0.916 0.8676 0.8936 0.9084 0.836 0.8248 0.8586 0.9331 0.8645
0.067 0.0605 0.0608 0.0645 0.1993 0.1934 0.0676 0.0642 0.0659 0.0667
0.0678 0.0703 0.0669 0.0722 0.2313 0.214 0.0729 0.0735 0.079 0.073
1.1216 1.1564 1.1529 1.0533 1.0638 1.0173 0.9904 1.1206 1.1591 1.0884
1.0125 1.0435 0.9225 0.9147 1.0744 1.0442 0.9366 0.9563 1.0674 1.019
0.9303 0.9958 0.8998 0.8451 1.0442 1.0953 0.8838 0.9349 0.9681 0.9165
0.8916 0.9006 0.8018 0.8868 1.1446 1.1519 0.8886 0.7902 0.7899 0.7992
0.9271 0.9079 0.7607 0.658 0.8964 0.8905 0.6292 0.7561 0.8699 0.8469
0.634 0.6959 0.67 0.6823 0.9721 0.978 0.6356 0.6177 0.6865 0.6003
0.6196 0.6224 0.6459 0.6259 0.9371 1.0059 0.7224 0.7241 0.7133 0.6671
0.4467 0.5314 0.5453 0.6472 1.0247 0.916 0.5473 0.5083 0.5423 0.5054
0.5358 0.5961 0.6233 0.6711 0.9104 0.8633 0.6852 0.6939 0.5923 0.5506
0.0893 0.0986 0.084 0.0904 0.3221 0.3103 0.0959 0.1142 0.124 0.1307
0.2309 0.2512 0.2482 0.1673 0.2175 0.2134 0.1764 0.2386 0.2203 0.2235
0.0702 0.0836 0.0719 0.0708 0.2336 0.2236 0.0757 0.0797 0.0821 0.0696
1.3458 1.3898 1.2814 1.2052 1.2192 1.2115 1.2094 1.2838 1.3929 1.3332
0.9968 1.0517 0.9595 0.8222 1.0134 0.9218 0.8093 0.9942 1.0348 1.0125
0.9312 0.9017 0.7405 0.6951 0.9822 0.9901 0.75 0.851 0.9954 0.9828
0.6609 0.6424 0.604 0.6596 0.976 0.9402 0.6008 0.631 0.6874 0.7561
0.9898 1.0225 0.9207 0.8919 1.2708 1.2277 0.9812 1.0252 1.0675 1.0214
0.5899 0.7368 0.8706 0.9752 1.2822 1.2954 0.972 0.9436 0.8011 0.6873
0.8988 0.9081 0.8512 0.8147 1.1584 1.0239 0.728 0.8022 0.9142 0.8946
0.8339 0.9019 0.9074 0.8376 1.0035 1.0109 0.8815 0.9562 0.9287 0.8927
0.9166 0.9849 0.9603 0.8314 1.039 1.042 0.858 0.9034 0.9226 0.8371
0.7762 0.7868 0.8305 0.8624 0.9124 0.89 0.8321 0.8012 0.7952 0.7648
0.2365 0.2148 0.193 0.1545 0.2441 0.2606 0.213 0.2573 0.3195 0.359
0.0695 0.0691 0.0639 0.0658 0.2161 0.1993 0.065 0.0701 0.0773 0.0743
1.1515 1.2075 1.1033 1.0248 1.0371 1.0805 1.0742 1.0972 1.0983 1.0581
0.9048 1.0303 0.895 0.9106 1.1278 1.0382 0.8561 0.9087 0.9756 0.8935
0.9325 0.9671 0.9351 0.9064 1.1465 1.0363 0.8598 0.9217 0.995 0.9633
0.8951 0.9799 0.9743 0.9435 1.1715 1.1437 0.9737 1.0575 1.0177 0.9682
0.8857 0.9308 0.9381 0.8876 1.1668 1.0773 0.836 0.8865 0.8643 0.8238
0.998 1.0179 1.0127 0.977 1.1742 1.2213 1.0821 1.1205 1.123 1.0891
0.9947 1.1035 1.0791 1.0213 1.2017 1.2468 1.0676 1.1365 1.109 0.9926
0.726 0.566 0.4225 0.2831 0.5527 0.4681 0.2616 0.49 0.6536 0.7763
0.9394 0.9167 0.8497 0.8173 0.934 0.9606 0.8136 0.9225 0.9903 0.9535
0.0744 0.0939 0.0837 0.0836 0.354 0.3283 0.0784 0.0922 0.0921 0.0798
0.0649 0.0698 0.0686 0.0701 0.1936 0.1945 0.0742 0.0753 0.0699 0.0668
0.0604 0.0708 0.0643 0.0627 0.1669 0.1631 0.0666 0.0805 0.081 0.0653
0.9664 1.019 0.9961 0.9715 1.0466 1.042 0.9769 1.0529 1.0474 0.9888
0.946 0.9741 0.9723 0.9128 1.0216 1.0532 0.9274 0.9819 0.9843 0.9143
0.8631 0.9543 1.0217 0.9181 1.1406 1.1367 0.9205 0.9503 0.9194 0.8502
1.0044 1.0128 0.9847 0.8948 1.0788 1.1594 0.9508 1.0286 1.0376 0.9659
1.0323 1.0999 1.1394 1.0744 1.266 1.1835 1.0623 1.121 1.151 1.0945
0.9719 0.9835 0.96 0.9047 1.1227 1.1025 0.9642 1.0718 1.0266 0.9597
0.9905 1.0654 0.9594 0.8694 1.0194 1.1228 0.9713 1.0392 1.024 0.9469
0.8568 0.8279 0.8216 0.7954 0.9966 1.0278 0.8342 0.8404 0.8588 0.8299
0.6503 0.7216 0.7049 0.7277 0.9544 0.8731 0.725 0.7372 0.7576 0.7139
0.5754 0.5769 0.5443 0.5479 0.7289 0.7682 0.5793 0.5471 0.5324 0.5364
0.5902 0.6045 0.5987 0.603 0.6788 0.6556 0.6347 0.6664 0.6097 0.5995
0.0674 0.0666 0.065 0.0712 0.2168 0.2044 0.0793 0.0746 0.073 0.0727
0.4881 0.5272 0.5336 0.506 0.5467 0.5413 0.4894 0.5049 0.4842 0.4723
0.497 0.4793 0.4981 0.4996 0.5502 0.5371 0.5013 0.465 0.4873 0.471
0.5174 0.5253 0.5322 0.5069 0.6686 0.6971 0.5254 0.5601 0.5376 0.5181
0.771 0.7864 0.755 0.719 0.9282 0.9075 0.7049 0.7606 0.746 0.7223
0.576 0.6237 0.6083 0.6029 0.8318 0.8039 0.6049 0.6073 0.617 0.5711
0.5183 0.5246 0.5572 0.5443 0.8016 0.8018 0.5498 0.534 0.5407 0.5332
0.8483 0.8225 0.7904 0.7402 0.9665 0.9392 0.7299 0.8224 0.8716 0.9138
0.3587 0.372 0.3918 0.4128 0.701 0.6065 0.3323 0.3245 0.3451 0.3841
0.3647 0.405 0.4137 0.3946 0.6355 0.6077 0.3753 0.3943 0.394 0.3752
0.3603 0.3404 0.3545 0.3863 0.6247 0.5967 0.3742 0.356 0.3127 0.3419
0.2603 0.2546 0.2898 0.2951 0.3067 0.3122 0.2696 0.2665 0.279 0.2466
0.3044 0.3272 0.3593 0.3746 0.4329 0.4143 0.3549 0.3328 0.3116 0.296
0.3649 0.3552 0.3887 0.3741 0.4166 0.4191 0.3674 0.3705 0.3682 0.3529
0.3402 0.3541 0.3316 0.3269 0.3875 0.3571 0.3158 0.3186 0.3146 0.3115
0.3397 0.3705 0.362 0.3434 0.435 0.4151 0.3675 0.3859 0.3882 0.3601
0.3297 0.382 0.4 0.3874 0.3894 0.4222 0.3925 0.3756 0.3651 0.3486
0.4275 0.4266 0.4655 0.3926 0.5354 0.5495 0.4258 0.4441 0.4594 0.4131
0.4149 0.4699 0.4402 0.4183 0.4408 0.3894 0.3784 0.4157 0.4286 0.4102
0.3238 0.3653 0.3629 0.3242 0.454 0.4512 0.3257 0.3375 0.3638 0.3299
0.3435 0.3765 0.3522 0.3469 0.4427 0.4583 0.3485 0.3581 0.3416 0.36
0.3251 0.3682 0.4017 0.3478 0.4159 0.4287 0.3548 0.3626 0.3608 0.3651
0.3297 0.3712 0.3498 0.328 0.3658 0.4097 0.3628 0.3849 0.3666 0.3227
0.2794 0.3249 0.3246 0.3335 0.317 0.3935 0.3294 0.2875 0.2861 0.2745
0.2525 0.2627 0.2513 0.2441 0.3119 0.3298 0.2717 0.2733 0.2802 0.2611
5;2 6;2 5;1 4;1 3;1 2;1 1;1 2;0 3;0 4;0
0.8128 0.8905 0.8642 0.871 0.8661 0.8105 0.7554 0.8472 0.8551 0.852
0.7111 0.7426 0.7074 0.7867 0.8469 0.8155 0.7628 0.8931 0.9137 0.8369
0.5848 0.6361 0.5191 0.5518 0.7116 0.8233 0.864 0.8744 0.6891 0.5118
0.4509 0.3764 0.5089 0.5824 0.6781 0.7461 0.8912 0.8108 0.6848 0.6071
0.4855 0.4639 0.4473 0.4876 0.4283 0.4913 0.6152 0.5483 0.4702 0.4994
0.6057 0.8109 0.6197 0.5703 0.5335 0.4738 0.5912 0.5415 0.5762 0.6796
0.496 0.6811 0.497 0.3849 0.3493 0.3104 0.3683 0.4441 0.4461 0.5791
0.1228 0.1828 0.1086 0.1305 0.2263 0.3276 0.3962 0.3812 0.2153 0.1485
0.2996 0.251 0.271 0.2928 0.2549 0.2013 0.2067 0.1813 0.2303 0.2665
0.5507 0.6479 0.5637 0.4995 0.4404 0.3411 0.3886 0.4566 0.5149 0.5488
0.0635 0.1214 0.0729 0.0614 0.0613 0.0648 0.1158 0.1147 0.0873 0.0938
0.0595 0.0908 0.0654 0.0582 0.0598 0.0634 0.1103 0.1104 0.0835 0.0903
0.7924 0.8638 0.9055 0.8265 0.8736 0.913 0.9336 0.9009 0.8746 0.8857
0.9802 1.0429 0.9547 0.9429 0.9719 0.9211 0.8592 0.9058 0.9145 0.9632
0.851 0.8836 0.9266 0.8915 0.9184 0.9483 0.9813 1.0048 0.8478 0.8334
0.7516 1.0002 0.8626 0.7806 0.8165 0.7376 0.794 0.8722 0.8234 0.9527
0.6594 0.9542 0.7 0.6542 0.6402 0.6155 0.7493 0.7865 0.7652 0.8527
0.5948 0.7164 0.6585 0.7817 0.9061 1.0087 1.0951 1.0233 0.8881 0.885
0.4859 0.5544 0.4809 0.5699 0.7099 0.7216 0.7483 0.8785 0.803 0.6911
0.3773 0.4644 0.399 0.4472 0.4737 0.4627 0.5555 0.6097 0.5021 0.5748
0.404 0.6412 0.4314 0.2959 0.2695 0.2028 0.2442 0.3094 0.3204 0.4242
0.8274 0.9021 0.8184 0.7963 0.8485 0.8265 0.7959 0.8475 0.8073 0.8118
0.0739 0.1471 0.0823 0.0642 0.0626 0.0671 0.1125 0.1168 0.0858 0.0955
0.0637 0.125 0.0735 0.066 0.0692 0.0682 0.1166 0.1202 0.0867 0.0924
1.0583 1.0061 1.0799 1.1022 1.0221 0.9524 0.9503 0.9724 1.0199 1.1857
0.941 1.0384 0.8755 0.8719 0.9327 0.9247 0.9585 0.8936 0.8583 0.8426
0.8935 1.0197 0.8446 0.8396 0.876 0.9095 0.9498 0.9363 0.8834 0.912
0.8219 0.9142 0.7995 0.7032 0.7007 0.7847 0.9069 0.8514 0.7619 0.824
0.8338 1.1422 0.8833 0.7527 0.742 0.682 0.7595 0.8669 0.7676 0.88
0.5208 0.7756 0.6146 0.5817 0.6467 0.6312 0.755 0.8043 0.7794 0.826
0.6759 0.9048 0.7182 0.6235 0.6537 0.7529 0.8922 0.8749 0.745 0.8073
0.4217 0.6007 0.5713 0.5247 0.5259 0.538 0.6487 0.7513 0.6473 0.6825
0.5475 0.6141 0.4649 0.5022 0.5946 0.6587 0.7044 0.6962 0.6122 0.5676
0.1124 0.2587 0.1462 0.1104 0.1146 0.1182 0.1763 0.189 0.1377 0.1679
0.1933 0.2272 0.1652 0.1584 0.1502 0.1432 0.1558 0.1476 0.1266 0.1396
0.0646 0.1232 0.0724 0.0644 0.0733 0.0747 0.1186 0.1194 0.0859 0.1022
1.2586 1.2191 1.2427 1.3263 1.2924 1.3004 1.2516 1.1858 1.2411 1.3258
0.9313 1.0573 1.0286 1.036 1.014 0.9702 0.9837 1.0131 1.0431 1.1205
0.9019 1.0763 0.9858 0.9209 0.9409 0.8812 0.9865 0.9819 0.9457 1.0531
0.7176 0.9957 0.782 0.7319 0.7427 0.6349 0.7228 0.8361 0.8118 0.8765
1.003 1.2668 1.081 1.0248 1.0156 0.9793 1.0879 1.1077 1.0451 1.1628
0.5335 0.7767 0.7113 0.7071 0.882 0.9778 1.1146 1.0977 1.0158 0.9598
0.8987 1.1683 0.9111 0.854 0.8146 0.7221 0.7846 0.9112 0.9649 1.0424
0.8926 1.1044 0.9132 0.9075 0.9079 0.8961 0.9246 0.9217 0.9374 0.9673
0.8454 1.0871 0.8424 0.8036 0.8328 0.8721 0.9355 0.8919 0.8258 0.8921
0.6732 0.5811 0.7349 0.8297 0.8248 0.79 0.8183 0.7889 0.7611 0.8238
0.4353 0.6347 0.5449 0.4226 0.4106 0.3307 0.2724 0.4818 0.4503 0.4691
0.0658 0.1269 0.0799 0.0667 0.0691 0.0654 0.0947 0.1117 0.0843 0.0981
1.0474 1.1531 1.065 1.0078 1.0209 1.043 1.0691 0.9545 1.0101 1.0682
0.8044 0.9709 0.9407 0.8963 0.9329 0.8891 0.9371 0.9858 0.9599 1.0211
0.9108 1.0879 0.9132 0.9039 0.9056 0.9242 0.9574 0.9731 0.922 0.9992
0.8837 1.0504 0.8929 0.8967 0.9147 0.9509 1.0374 0.9766 0.8987 0.9746
0.8194 1.0744 0.8245 0.7278 0.7708 0.7824 0.9282 0.9374 0.8354 0.864
0.936 0.9872 1.0312 1.0617 1.093 1.1182 1.1146 1.0942 1.0912 1.1405
0.8082 0.9389 0.8414 0.944 1.0496 1.063 1.0733 1.0969 1.0513 1.0625
0.8366 1.1863 0.8139 0.7576 0.7363 0.5482 0.4915 0.7942 0.8289 0.9035
0.9372 1.0751 0.9271 0.888 0.9146 0.8932 0.8633 0.9395 0.8808 0.9185
0.1059 0.3407 0.1499 0.0745 0.0732 0.0723 0.1641 0.1753 0.1465 0.2297
0.0716 0.1414 0.0894 0.0673 0.072 0.075 0.1007 0.1123 0.0844 0.1042
0.0647 0.1152 0.0774 0.0623 0.0689 0.0691 0.0875 0.1064 0.0808 0.0933
1.0129 1.102 0.9102 0.8726 0.9269 0.9296 0.891 0.7884 0.766 0.8295
0.9189 1.0769 0.9334 0.8757 0.9191 0.9176 0.9438 0.9639 0.9436 1.0245
0.9541 1.2587 0.9732 0.8027 0.8242 0.9607 0.9694 0.992 0.8878 0.9741
0.9575 1.1884 0.9855 0.9279 1.0063 0.9993 1.0794 1.0353 0.9966 1.0535
1.0427 1.1737 1.0762 1.0552 1.1017 1.0443 1.0318 1.1007 1.0904 1.1683
0.9169 1.1149 1.0942 1.014 1.0331 1.0336 1.0644 1.0594 1.0591 1.1407
0.9152 1.0572 0.9658 0.9316 1.0183 1.0215 1.072 1.0751 1.051 1.0833
0.8683 1.0695 0.851 0.778 0.8066 0.8364 0.8833 0.9151 0.8899 0.9402
0.6848 0.8569 0.6802 0.6917 0.7734 0.7723 0.7709 0.8271 0.7772 0.7688
0.5496 0.6581 0.5065 0.5146 0.4542 0.4191 0.5066 0.4842 0.549 0.6092
0.5799 0.6739 0.6563 0.6194 0.683 0.6844 0.6528 0.7198 0.6921 0.6521
0.0755 0.12 0.1064 0.0877 0.0846 0.0903 0.1236 0.1497 0.1172 0.1396
0.5082 0.6197 0.5242 0.4819 0.476 0.4724 0.4365 0.4925 0.5229 0.5219
0.4641 0.5546 0.4803 0.4711 0.4746 0.4825 0.4858 0.492 0.4668 0.5085
0.515 0.6482 0.5454 0.5076 0.5231 0.5333 0.5502 0.5844 0.5512 0.5822
0.7287 0.963 0.7761 0.7453 0.7856 0.7685 0.8178 0.8728 0.822 0.8799
0.5943 0.8428 0.6436 0.5746 0.591 0.5704 0.6327 0.6615 0.6471 0.7388
0.552 0.9169 0.6742 0.5262 0.5306 0.4847 0.5717 0.6769 0.6408 0.743
1.0413 1.3936 1.1279 0.9115 0.9299 0.9219 0.894 1.1161 1.0517 1.0912
0.485 0.9069 0.5989 0.3922 0.3459 0.3358 0.4333 0.59 0.5084 0.651
0.4029 0.6663 0.4449 0.338 0.3513 0.3853 0.4415 0.4543 0.3978 0.4718
0.4051 0.5953 0.4374 0.315 0.3344 0.3353 0.3958 0.4571 0.3967 0.4934
0.2468 0.3731 0.2672 0.2552 0.2788 0.2752 0.2875 0.3256 0.2998 0.2885
0.2779 0.2999 0.2892 0.2719 0.3048 0.3394 0.363 0.3655 0.3241 0.3224
0.3701 0.4575 0.3336 0.3017 0.3225 0.3396 0.3514 0.3346 0.3262 0.3584
0.3291 0.3723 0.3119 0.305 0.3195 0.3351 0.3506 0.3189 0.3136 0.3637
0.3548 0.4622 0.3577 0.3361 0.3595 0.3651 0.3747 0.3938 0.3762 0.3582
0.3555 0.391 0.3369 0.3129 0.3674 0.3918 0.3748 0.3894 0.3468 0.3496
0.3977 0.4575 0.3835 0.3806 0.4282 0.4571 0.4462 0.4673 0.4349 0.4689
0.4074 0.4774 0.3759 0.3308 0.3954 0.388 0.375 0.3871 0.3514 0.3678
0.3366 0.4612 0.3515 0.3143 0.3298 0.3306 0.3471 0.3367 0.3611 0.4083
0.377 0.5355 0.3838 0.3584 0.3312 0.3836 0.3863 0.3605 0.3529 0.4297
0.3388 0.4356 0.3309 0.3147 0.3388 0.3557 0.3733 0.3651 0.3605 0.4068
0.3246 0.3809 0.3258 0.3177 0.344 0.3383 0.3395 0.3375 0.3101 0.3393
0.2858 0.3065 0.3113 0.2486 0.2763 0.3001 0.3125 0.3136 0.2823 0.2723
0.2399 0.2825 0.2884 0.2792 0.2776 0.2709 0.2826 0.3065 0.2902 0.3017
 Zanamivir
25 12.5 6.25 3.125 1.5625 0.78 0.39 0.195
LMB 1 2 3 4 5 6 7 8
80 A 0.8273 0.7863 0.6786 0.6681 0.5326 0.5846 0.481 0.3402
40 B 0.886 0.9764 0.9263 0.8676 0.7931 0.9061 0.7643 0.5876
20 C 1.0411 0.9791 0.965 0.9276 0.9241 0.7761 0.7855 0.6851
10 D 1.2587 1.0113 0.9862 0.8367 1.0864 0.9244 0.9923 0.9459
5 E 1.0968 0.9953 1.0027 0.9985 0.9722 1.0611 1.0437 0.7536
2.5 F 1.007 0.9913 1.0034 1.0469 1.1284 1.0449 0.973 0.8748
1.25 G 0.547 0.5308 0.6068 0.8354 0.7097 0.6877 0.9905 0.5488
0 H 0.3856 0.3604 0.4012 0.3841 0.4872 0.4196 0.4018 0.4115
LMB BLANK - 25 12.5 6.25 3.125 1.5625 0.78 0.39 0.195
80 A 0.7465 0.7055 0.5978 0.5873 0.4518 0.5038 0.4002 0.2594
40 B 0.8052 0.8956 0.8455 0.7868 0.7123 0.8253 0.6835 0.5068
20 C 0.9603 0.8983 0.8842 0.8468 0.8433 0.6953 0.7047 0.6043
10 D 1.1779 0.9305 0.9054 0.7559 1.0056 0.8436 0.9115 0.8651
5 E 1.016 0.9145 0.9219 0.9177 0.8914 0.9803 0.9629 0.6728
2.5 F 0.9262 0.9105 0.9226 0.9661 1.0476 0.9641 0.8922 0.794
1.25 G 0.4662 0.45 0.526 0.7546 0.6289 0.6069 0.9097 0.468
0 H 0.3048 0.2796 0.3204 0.3033 0.4064 0.3388 0.321 0.3307

BLANK - Zanamivir (uM)

LMB 0 0.02 0.0487 0.0975 0.195 0.39 0.78 1.5625
LMB 80ng/ 0.0146 0.0194 0.3586 0.2104 0.2594 0.4002 0.5038 0.4518
LMB 40 ng 0.0316 0.0328 0.7883 0.3336 0.5068 0.6835 0.8253 0.7123
LMB 20 ng 0.0374 0.1225 0.1099 0.6047 0.6043 0.7047 0.6953 0.8433
LMB 10 ng 0.0381 0.2133 0.7012 0.8632 0.8651 0.9115 0.8436 1.0056
LMB 5 ng/ 0.026 0.041 0.1321 0.8337 0.6728 0.9629 0.9803 0.8914
LMB 2.5 ng 0.0523 0.5208 0.5455 0.7019 0.794 0.8922 0.9641 1.0476
LMB 1.25 n 0.2619 0.2241 0.3845 0.4175 0.468 0.9097 0.6069 0.6289
LMB 0 ng/ 0.209 0.2446 0.2852 0.3197 0.3307 0.321 0.3388 0.4064
Well Mean
A1 0.8273
A2 0.7863
A3 0.6786
A4 0.6681
A5 0.5326
A6 0.5846
A7 0.481
A8 0.3402
A9 0.2912
A10 0.4394
A11 0.1002
A12 0.0954
B1 0.886
B2 0.9764
B3 0.9263
B4 0.8676
B5 0.7931
B6 0.9061
B7 0.7643
B8 0.5876
B9 0.4144
B10 0.8691
B11 0.1136
B12 0.1124
C1 1.0411
C2 0.9791
C3 0.965
C4 0.9276
C5 0.9241
C6 0.7761
C7 0.7855
C8 0.6851
C9 0.6855
C10 0.1907
C11 0.2033
C12 0.1182
D1 1.2587
D2 1.0113
D3 0.9862
D4 0.8367
D5 1.0864
D6 0.9244
D7 0.9923
D8 0.9459
D9 0.944
D10 0.782
D11 0.2941
D12 0.1189
E1 1.0968
E2 0.9953
E3 1.0027
E4 0.9985
E5 0.9722
E6 1.0611
E7 1.0437
E8 0.7536
E9 0.9145
E10 0.2129
E11 0.1218
E12 0.1068
F1 1.007
F2 0.9913
F3 1.0034
F4 1.0469
F5 1.1284
F6 1.0449
F7 0.973
F8 0.8748
F9 0.7827
F10 0.6263
F11 0.6016
F12 0.1331
G1 0.547
G2 0.5308
G3 0.6068
G4 0.8354
G5 0.7097
G6 0.6877
G7 0.9905
G8 0.5488
G9 0.4983
G10 0.4653
G11 0.3049
G12 0.3427
H1 0.3856
H2 0.3604
H3 0.4012
H4 0.3841
H5 0.4872
H6 0.4196
H7 0.4018
H8 0.4115
H9 0.4005
H10 0.366
H11 0.3254
H12 0.2898

End Time: 2011/02/12 14:27:39

Movement Move Plate Out

0.0975 0.0487 0.02 0
9 10 11 12
0.2912 0.4394 0.1002 0.0954 Zanamivir-LMB synergestic activity against
0.4144 0.8691 0.1136 0.1124 1 LMB 2.5
0.6855 0.1907 0.2033 0.1182 0.9 ng/ml
LMB 0
0.944 0.782 0.2941 0.1189 0.8 ng/ml
0.9145 0.2129 0.1218 0.1068 0.7

Absorbance (560nm)
0.7827 0.6263 0.6016 0.1331 0.6
0.4983 0.4653 0.3049 0.3427 0.5
0.4005 0.366 0.3254 0.2898 0.4
0.0975 0.0487 0.02 0 0.3
0.2104 0.3586 0.0194 0.0146 0.2
0.1
0.3336 0.7883 0.0328 0.0316
0
0.6047 0.1099 0.1225 0.0374
0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4
0.8632 0.7012 0.2133 0.0381
Zanamivir Conc (μM)
0.8337 0.1321 0.041 0.026
0.7019 0.5455 0.5208 0.0523
0.4175 0.3845 0.2241 0.2619 Zanamivir-LMB synergestic activity aga
0.3197 0.2852 0.2446 0.209 1.4

1.2

1
Absorbance (560nm)

3.125 6.25 12.5 25

0.8
0.5873 0.5978 0.7055 0.7465 A
0.7868 0.8455 0.8956 0.8052 B 0.6
0.8468 0.8842 0.8983 0.9603 C
0.7559 0.9054 0.9305 1.1779 D 0.4
0.9177 0.9219 0.9145 1.016 E
0.9661 0.9226 0.9105 0.9262 F 0.2
0.7546 0.526 0.45 0.4662 G
0
0.3033 0.3204 0.2796 0.3048 H
0 02 7 5 5 39 78 5 5 25 .5
0. 48 97 19 0. 0. 62 12 6. 12
0.
0
0.
0 0. 1.
5 3.

Zanamivir Conc (μM)

Zanamivir-LMB synergestic activity against AWS

1.2
LMB
1 80ng/ml
LMB 40
Absorbance (560nm)

0.8 ng/ml
LMB 20
ng/ml
0.6 LMB 10
ng/ml
0.4 LMB 5
ng/ml
LMB 2.5
0.2 ng/ml
LMB 1.25
0 ng/ml
0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4
 Absorbance (560nm
0.8 ng/ml
LMB 20
ng/ml
0.6 LMB 10
ng/ml
0.4 LMB 5
ng/ml
LMB 2.5
0.2 ng/ml
LMB 1.25
0 ng/ml
0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4
Zanamivir Conc (μM)

Zanamivir-LMB synergestic activity against AWSN

1.4
LMB
1.2 80ng/ml
LMB 40
1

Absorbance (560nm)
ng/ml
LMB 20
0.8 ng/ml
LMB 10
0.6 ng/ml
LMB 5
0.4 ng/ml
LMB 2.5
0.2 ng/ml
LMB 1.25
0 ng/ml
0 5 10 15 20 25 30
Zanamivir Conc (μM)
 ir-LMB synergestic activity against AWSN/33
LMB 2.5
ng/ml
LMB 0
ng/ml

0.2 0.25 0.3 0.35 0.4

r Conc (μM)

MB synergestic activity against AWSN/33

LMB 80ng/ml
LMB 40
ng/ml
LMB 20
ng/ml
LMB 10
ng/ml
LMB 5 ng/ml
LMB 2.5
ng/ml
LMB 1.25
ng/ml
LMB 0 ng/ml
Row 34
39 78 5 5 25 .5 25
0. 0. 62 12 6. 12
1.
5 3.

mivir Conc (μM)

rgestic activity against AWSN/33

LMB
80ng/ml
LMB 40
ng/ml
LMB 20
ng/ml
LMB 10
ng/ml
LMB 5
ng/ml
LMB 2.5
ng/ml
LMB 1.25
ng/ml
.25 0.3 0.35 0.4
 ng/ml
LMB 20
ng/ml
LMB 10
ng/ml
LMB 5
ng/ml
LMB 2.5
ng/ml
LMB 1.25
ng/ml
.25 0.3 0.35 0.4
(μM)

rgestic activity against AWSN/33

LMB
80ng/ml
LMB 40
ng/ml
LMB 20
ng/ml
LMB 10
ng/ml
LMB 5
ng/ml
LMB 2.5
ng/ml
LMB 1.25
ng/ml
20 25 30
nc (μM)
 m
A

5
0
N
r

c
s
b

b
n

)
o

6
e
(

Z
a
n
a
m
i
v
i
r
C
o
n
c
(
μ
M
)
 ZANAMIVIR
0 0.024 0.049 0.098 0.195 0.391 0.7811 1.563
LMB 80 ng 0.2161 0.2308 0.2774 0.2952 0.3056 0.2887 0.3265 0.3829
LMB 40 ng 0.2704 0.2225 0.3087 0.3405 0.3449 0.7882 0.4961 0.5684
LMB 20 ng 0.0593 0.545 0.5014 0.6784 0.7784 0.9043 0.9298 1.006
LMB 10 ng 0.0288 0.0379 0.0554 0.8188 0.5296 0.8941 0.9699 0.7696
LMB 5 ng/ 0.0401 0.2559 0.7023 0.7702 0.8157 0.7496 0.7569 0.9033
LMB 2.5 ng 0.0451 0.1215 0.0859 0.5741 0.5067 0.6284 0.581 0.6979
LMB 1.25 n 0.0401 0.0358 0.7815 0.2623 0.4197 0.6538 0.7774 0.6281
LMB 0 ng/ 0.0281 0.034 0.379 0.2055 0.2631 0.3307 0.4981 0.4774

ZANAMIVIR-LMB SYNERGESTIC ACTIVITY ON A/WSN/33 VIRUS

1
0.9
0.8
Absorbance (A560

0.7
0.6
LMB 80 ng/ml
0.5
LMB 40 ng/ml
0.4 LMB 20 ng/ml
0.3 LMB 10 ng/ml
nm)

0.2
0.1
0
0 5 10 15 20 25
Zanamivir conc. (μM)

ZANAMIVIR-LMB SYNERGESTIC ACTIVITY ON A/WSN/33 VIRUS

1.2

0
1
0.024
Absorbance (560 nm)

0.049
0.8 0.098
0.195
0.6 0.391
0.7811
1.563
0.4
3.125
6.25
0.2 12.5
25
0
0 1 2 3 4 5 6 7 8 9 10
LMB (ng/ml)
 3.125

A
6.25
0.2 12.5
25
0
0 1 2 3 4 5 6 7 8 9 10
LMB (ng/ml)

1.2

LMB 80 ng/ml
0.8
LMB 40 ng/ml
LMB 20 ng/ml
0.6 LMB 10 ng/ml
LMB 5 ng/ml
LMB 2.5 ng/ml
0.4
LMB 1.25 ng/ml
LMB 0 ng/ml
0.2

0
0 5 10 15 20 25 30
 ZANAMIVIR-LMB SYNERGESTIC ACT
A/WSN/33 VIRUS
3.125 6.25 12.5 25 0.9
0.2923 0.3033 0.2719 0.2837 0.8

Absorbance (A560 nm)

0.6968 0.5159 0.4376 0.441 0.7
0.9089 0.8578 0.8813 0.8666 0.6
0.8683 0.8353 0.8526 0.9789 0.5
0.6241 0.7869 0.8659 1.1368 0.4
0.8094 0.831 0.8645 0.9347 0.3
0.6865 0.9033 0.8756 0.7965 0.2
0.6397 0.6183 0.7147 0.7408 0.1
0
0 0.02 0.04 0.06 0.08 0.1 0.12 0.14 0.16 0.18 0.
IRUS
Zanamivir conc. (μM)

ZANAMIVIR-LMB SYNERGESTIC ACTIVITY ON A/WSN/33 VIRUS

1
LMB 80 ng/ml 0.9
Absorbance (A560 nm)

LMB 40 ng/ml 0.8

LMB 20 ng/ml
0.7 LMB 80 ng/ml
LMB 10 ng/ml
0.6
LMB 20 ng/ml
0.5
LMB 5 ng/ml
0.4
0.3 LMB 2.5 ng/ml
5
0.2 LMB 0 ng/ml
0.1
0
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1
Zanamivir conc. (μM)

1.2

1
Absorbance (A560

0.8 LMB 80 ng/ml

LMB 40 ng/ml
0.6 LMB 20 ng/ml
LMB 10 ng/ml
0.4 LMB 5 ng/ml
LMB 2.5 ng/ml
nm)

0.2 LMB 1.25 ng/ml

LMB 0 ng/ml
0
0 4 9 8 5 1 1 3 5 5 .5 25
02 04 09 19 39 81 56 12 6.2 12
0. 0. 0. 0. 0. 0.7 1. 3.
Zanamivir conc. (μM)
 LMB 10 ng/ml

Absorba
0.4 LMB 5 ng/ml
LMB 2.5 ng/ml

nm)
0.2 LMB 1.25 ng/ml
LMB 0 ng/ml
0
0 4 9 8 5 1 1 3 5 5 .5 25
02 04 09 19 39 81 56 12 6.2 12
0. 0. 0. 0. 0. 0.7 1. 3.
Zanamivir conc. (μM)

1.2

1
Zan 0
0.8 Zan 0.024
Zan 0.049
0.6 Zan 0.098
Zan o.195
0.4 Zan 0.391
Zan 0.7811
0.2 Zan 1.563
Zan 3.125
0 Zan 6.25
Zan 12.5
Zan 25
 LMB SYNERGESTIC ACTIVITY ON
RUS

LMB 5 ng/ml
LMB 2.5 ng/ml
LMB 0 ng/ml

0.08 0.1 0.12 0.14 0.16 0.18 0.2

Zanamivir conc. (μM)

N/33 VIRUS

LMB 80 ng/ml
LMB 20 ng/ml
LMB 5 ng/ml
LMB 2.5 ng/ml
LMB 0 ng/ml

LMB 80 ng/ml
LMB 40 ng/ml
LMB 20 ng/ml
LMB 10 ng/ml
LMB 5 ng/ml
LMB 2.5 ng/ml
LMB 1.25 ng/ml
LMB 0 ng/ml
LMB 10 ng/ml
LMB 5 ng/ml
LMB 2.5 ng/ml
LMB 1.25 ng/ml
LMB 0 ng/ml