APPLICATIONS OF ANIMAL CELL CULTURE

Application animal cell culture for study of gene expression Cell culture based vaccine production Recombinant proteins Production of monoclonal antibodies Tissue culture application

FOR STUDY OF GENE EXPRESSION

Animal cell can be cultured after being transduced with nucleic acids. The cells in the culture would behave in almost the same way as they would behave in an animal body. So this would help us to understand the change in gene gene expression due to the culture genotype.

 FOR EXAMPLE If you doubt that insulin is induced produced by the INS gene, you can introduce the gene into a compatible host cell and allow the host to survive in an appropriate culture to demonstrate the production of insulin in the medium.

PRODUCTION OF MONOCLONAL ANTIBODIES USING HYBRIDOMA TECHNOLOGY

FIG SHOWING THE STEPS INVOLVED IN THE PRODUCTION OF MONOCLONAL ANTIBODIES

Applications of monoclonal antibodies Mabs are available for unequivocal classification of blood. Mabs applied for clear and decisive detection of pathogens involved in diseases. Mab can used for an accurate detection of specific chromosomes of given a species

 An important marker antigen prasent in most tumours is carcinoembryonic antigen (CEA) . Mabs have been produced for CEA and some other tumour associated antigens. Use of such Mabs in histochemical assays permits the identification of 1) Tumour cell type 2) Beningn and malignat nature of tumour 3) Early cases of metastasis

RECOMBINANT PROTIENS
Proteins produced by genes transferred by genetic engineering into selected host cells are called recombinant proteins. Since their production based on recombinant DNA technology Recombinant proteins an important component of biopharmaceuticals

 Large number of recombinant proteins are being produced in mammalian cell cultures. Some of which ,human growth hormone ,tissue plasminogen activator(tPA), erythroprotien and blood clotting factor VIII

FIG SHOWING THE PRODUCTION OF T-PA

Tissue Plasminogen activator (t-PA) was the first drug that was produced by the mammalian cell culture by using rDNA technology. The recombinant t-PA is safe and effective for dissolving blood clots in patients with heart diseases and thrombotic disorders.

TISSUE CULTURE APPLICATION