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Universal Science College

(B. Sc. Biochemistry)


First Semester - 2005

Internal Examination Full Marks: 100


Subject: Biochem 115 (Fundamentals of Biochemistry) Pass Marks: 45
Date: Feb. 6, 2005 Time: 3 hours

Candidates are required to give their answers in their own words as far as practicable. The figure in the
margin indicates full marks.
Group A

Answer any three questions. Questions are of equal value. 3 × 14 = 42


1. Enumerate principles of Molecular Logic of Life.
2. Define phylogenetic process. How protista and higher eukaryotes may have evolved from prokaryotes.
3. Enumerate and illustrate general types of chemical reactions in a cell.
4. Discuss the role of noncovalent interactions in assembly of macromolecules.

Group B
Answer any six questions. Questions are of equal value. 6 × 7 = 42
1 Explain with examples the hydrophobic interaction.
2 Describe the role of genetic mutation in evolution.
3. Describe chemical composition of biological membranes.
4 Explain the differences between and prokaryotic and eukaryotic cells.
5 Derive Henderson – Hesselbach equation.
6. Describe the bicarbonate buffer system.
7. Determine the weight in grams of sodium dihydrogen phosphate (formula weight FW 138.01) and disodium
hydrogen phosphate ( FW 149.98 ) needed to prepare 1 liter of a standard buffer at pH 7.00 with a total phosphate
concentration of 0.100 M. ( pKa of dihydrogen phosphate = 6.86 and pKa of monohydrogen phosphate = 12.4 ).
8 In a hospital laboratory, a 10.0 ml sample of gastric juice , obtained several hours after a meal, was titrated with 0.1
M NaOH to neutrality; 7.2 ml of NaOH was required. The stomach contained no ingested food or drink, thus
assume that no buffers were present. What was the pH of gastric juice ?

Group – C
Answer any eight questions. Questions are of equal value. 8 × 2 = 16
1. Structure of ATP.
2. What is an open, isothermal system?
3. Different between tight junction and gap junction
4. Plasmids
5. Thylakoids
6. How the lysosomes provides a second line of defence against destruction of cytosolic macromolecules.
7. Stereospecificity
8. Enantiomers
9. Entropy
10. Structure of aspartic acid
11. Amphipathic compounds
12. Virions and bacteriophages.

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