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CH 395G BIO 395G MOL 395G

Fall 2004
Tuesday & Thursday 11-12:30
WEL 2.304

Instructors: Barrie Kitto and Marvin Hackert


Teaching Assistant : Larisa Watson
Dr. Marvin Hackert
Structural Molecular Biology, Protein Crystallography
Enzyme Mechanisms

PLP_Dependent Decarboxylases

http://www.cm.utexas.edu/hackert/
Dr Barrie Kitto

Immunoassays Contraceptives

http://www.cm.utexas.edu/kitto/Kittolabpage/
Course Schedule
Introductory Material
Use the class web site at:

cm.utexas.edu/CH395G/Kitto/for all announcements, exam dates, times and place and class
Class lecture note key points will also be posted there in PDF (Acrobat) format.

Use of University Libraries


http://www.lib.utexas.edu/
Chemistry Library WEL 2.132
Life Sciences Library MAI 220

Use of Internet:
From laboratories
Computer Center WEL 2.302
Limitations of internet
Journals on internet
Use of CHIME for 3D Structures

college.com/chem/biochem/GarrettGrisham/WebLinks/WebLinks.html Necessity to use Netscape 4.x as browse


CHIME and 3D Structures
• Use Mozilla or Netscape 4.x

• See www.boyerbiochem.com for some


examples
Lecture 1 Outline
Chapter 1
Small to large
--- from precursors to cells
The interior of cells
---crowded
Intracellular location of processes
Bioelements
Biopolymers
Ordering of informatiion
Chapter 2
Aqueous conditions
Water -- polar nature
Solvent properties
Hydrogen bonds
Acids and Bases
pH
Henderson Hasselbalch Equation
Constituents of Cells: E. coli
%
Water 70
Proteins 15
Nucleic Acids 7
Polysaccharides 3
Lipids 2
Monomers 2
Inorganic Ions 1
Hemoglobin
3 x 108 molecules per cell
5 x 106 cells/ml
Bioelements

Water: H,O
Organics: C,H,O,N,P,S
Ions: Na+, K+, Mg++, Ca++, Cl-
Trace Elements: Fe, Mn ,Co, Cu, Zn
Chapter 2
• Water
• Acids and Bases
• Ionization
Molarity of Water
1 liter of water weighs 1000 g
1 mole of water = 18 g

Therefore the molarity of water is


= 1000/18 M
= 55M
Acids, Bases and Buffers

pH, Titration, pKa, pKb,


physiological buffers
Ionization of Water
pH
• Because it is cumbersome to use
exponentials to describe the amount of
hydrogen ions present in a solution the pH
scale was adopted.

• pH = - log10 [H+]
• For example
if [H+] = 10-7M the pH = 7
Table 2-3 (bottom) Dissociation Constants and pK’s at
25°C of Some Acids in Common Laboratory Use as

Biochemical Buffers .
Page 45
For reviews of pH and buffers see

http://www.boyerbiochem.com/
and go to Concept Reviews