protected void btnassign_click(object sender, eventargs e)

{
int i = 0;
foreach (datagriditem di in dgagents.items)
{
checkbox chk1 = (checkbox)di.findcontrol("chk1");
if (chk1.checked == true)
{
i = i + 1;
}
}

if (i == 1)
{
//your code
}
else
{
page.registerstartupscript("as","<script>alert('you can assign one
property to one agent only')</script>");
}
}