n

SLep 1 Clucose ls
phosphorylaLed by A1 Lo
form a sugar phosphaLe
1he negaLlve charge of Lhe
phosphaLe prevenLs
passage of Lhe sugar
phosphaLe Lhrough Lhe
plasma membrane
Lrapplng glucose lnslde
Lhe cell
En azul, las dos
mitades de la
hexoquinasa libre,
en rojo, cuando se
unen con la
glucosa
SLep 2 A readlly reverslble
rearrangemenL of Lhe chemlcal
SLrucLure (lsomerlzaLlon)
moves Lhe carbonyl oxygen
from carbon 1 Lo carbon 2
formlng a keLose from an aldose
sugar
SLep 3 1he new hydroxyl
group on carbon 1 ls
phosphorylaLed by A1 ln
preparaLlon for Lhe formaLlon
of Lwo Lhreecarbon sugar
phosphaLes 1he enLry of
sugars lnLo glycolysls ls
conLrolled aL Lhls sLep Lhrough
regulaLlon of Lhe enzyme
phosphofrucLoklnase
SLep 4 1he slx
carbon sugar ls
cleaved Lo produce
Lwo Lhreecarbon
molecules Cnly Lhe
glyceraldehyde
3phosphaLe can
proceed lmmedlaLely
Lhrough glycolysls
SLep 3 1he oLher
producL of sLep 4
dlhydroxyaceLone
phosphaLe ls
lsomerlzed Lo form
glyceraldehyde
3phosphaLe
CenLro hecho de
ocho ho[as beLa
y rodeada de
ocho hellces
Lso es una
esLrucLura
relaLlvamenLe
comun llamada
el Lonel alfa
beLa
Se encuenLra en
la aldolasa la
enolasay en la
qulnasa del
plruvaLo
,ecanlsmo de la lsomerlzacln
a isomerizacin sucede por reaccin con Glu 165 (recibe protn) y His 85 (que dona, y
despus al revs.
SLep 6 1he Lwo molecules
of glyceraldehyde 3phosphaLe
are oxldlzed 1he energy
generaLlon phase of glycolysls
beglns as nAuP and a new
hlghenergy anhydrlde llnkage
Lo phosphaLe are formed
SLep 7 1he Lransfer
Lo Au of Lhe hlgh
energy phosphaLe
group LhaL was
generaLed ln sLep 6
forms A1
SLep 8 1he remalnlng
phosphaLe esLer llnkage ln
3phosphoglyceraLe
whlch has a relaLlvely low
free energy of hydrolysls
ls moved from carbon 3
Lo carbon 2 Lo form 2
phosphoglyceraLe
SLep 9 1he removal of
waLer from 2phosphoglyceraLe
creaLes a hlghenergy enol
phosphaLe llnkage
SLep 10 1he Lransfer Lo
Au of Lhe hlghenergy
phosphaLe group LhaL was
generaLed ln sLep 9 forms
A1 compleLlng
glycolysls
nL1 8LSuL1 Cl CL?CCL?SlS
Clycolysls