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12/8/11

Enzyme Cofactors

ENZYMES
Lock and Ke Theo Ca bo pep ida e

P o ein
Coen En me - NAD+ me Inhibi o En me Reg la ion

Elmh

College

Chemi Depa men Vi al ChemBook

Coen
Coen mes:

me

A nonp o ein componen of en me i called he cofac o . If he cofac o i o ganic, hen i i called a coen me. Coen me a e ela i el mall molec le compa ed o he p o ein pa of he en me. Man of he coen me a e de i ed f om i amin . The coen me make p a pa of he ac i e i e, ince i ho he coen me, he en me ill no f nc ion. In he g aphic on he lef i NAD+, he c e fo he

coen me, Nico inamide Adenine Din cleo ide. Nico inamide i f om he niacin i amin. The NAD+ coen me i in ol ed i h man pe of o ida ion eac ion he e alcohol a e con e ed o ke one o aldeh de . Vi amin niacin Coen me F nc ion o ida ion o h d ogen an fe o ida ion o h d ogen an fe Ace l g o p ca ie Me h l g o p an fe Aldeh de g o p an fe

nico inamide adenine din cleo ide (NAD+) fla in adenine din cleo ide (FAD) coen me A (CoA) me B-12

Click fo la ge image

ibofla in pan o henic acid

i amin B-12 coen

hiamin (B-1)

hiaminp opho pha e (TPP)

Alcohol Deh drogenase


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12/8/11

Enzyme Cofactors

Alcohol Deh drogenase Active Site: The NAD+ i ep e en ed a c an in he g aphic on he lef . The alcohol i ep e en ed b he pace filling ed, g a , and hi e a om . The eac ion i o con e e hanal, an aldeh de. he alcohol, e hanol, in o

Reac ion: CH3CH2OH + NAD+ ---> CH3CH=O + NADH + H+

Thi i an o ida ion eac ion and e l in he emo al of o h d ogen ion and o elec on hich a e added o he NAD+, con e ing i NADH and H+. Thi i he fi me aboli m of alcohol. Click fo la ge image The ac i e i e of ADH ha o eac ion in he o binding egion . The

coen me binding i e, he e NAD+ bind , and he b a e binding i e, he e he alcohol bind . Mo of he binding i e fo ep e en ed in g een. he NAD+ i h d ophobic a

Th ee ke amino acid in ol ed in he ca al ic o ida ion of alcohol o aldeh de and ke one . The a e e -48, phe 140, and phe 93. Alcohol Deh d ogena e - Chime in ne indo

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