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enzim katalase

enzim katalase

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Published by: Epik Mutiara on Jan 16, 2012
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LEMBAR KERJA

PRAKTIKUM BIOLOGI
Nama :
Kelas :
Kelompok :


KATABOLISME : MENGAMATI KERJA ENZIM


A. LANDASAN TEORI

Reaksi-reaksi metabolisme dalam sel berlangsung pada suhu yang sesuai dengan suhu
tubuh. Oleh karena itu, reaksi-reaksi metabolisme berlangsung lambat atau bahkan tidak dapat
berlangsung jika tidak sesuai dengan suhu tubuh. Untuk mengatasi hal tersebut, reaksi-reaksi
metabolisme membutuhkan katalisator berupa enzim. Enzim akan mempercepat reaksi karena
enzim dapat menurunkan energi aktivasi.

B. TUJUAN

Memahami dan mengetahui cara kerja dan faktor-faktor yang mempengaruhi kerja
enzim.

C. ALAT DAN BAHAN

1. ............................................ 9. ...............................................
2. ............................................ 10. ...............................................
3. ............................................ 11. ...............................................
4. ............................................ 12. ...............................................
5. ............................................ 13. ...............................................
6. ............................................ 14. ...............................................
7. ............................................ 15. ...............................................
8. ............................................ 16. ...............................................

D. CARA KERJA
1. Sediakan 8 tabung reaksi kemudian berilah label 1, 2, 3, 4, 5, 6, 7, 8.
2. Isilah tabung 1 : diisi ekstrak hati + 5 tetes larutan H
2
O
2

Isilah tabung 2 : diisi ekstrak hati + 5 tetes larutan H
2
O
2
, sebelumnya dipanaskan
Isilah tabung 3 : diisi ekstrak hati + 5 tetes larutan H
2
O
2 ,
sebelumnya + 10 tetes HCl
Isilah tabung 4 : diisi ekstrak hati + 5 tetes larutan H
2
O
2 ,
sebelumnya + 10 tetes NaOH
Isilah tabung 5 : diisi ekstrak bayam merah + 5 tetes larutan H
2
O
2

Isilah tabung 6 : diisi ekstrak bayam merah + 5 tetes larutan H
2
O
2
, sebelumnya
dipanaskan
Isilah tabung 7 : diisi ekstrak bayam merah + 5 tetes larutan H
2
O
2 ,
sebelumnya + 10 tetes
HCl
Isilah tabung 8 : diisi ekstrak bayam merah + 5 tetes larutan H
2
O
2 ,
sebelumnya + 10 tetes
NaOH
3. Hitunglah waktu yang dibutuhkan untuk terjadi gelembung udara
4. Untuk menguji adakah gelembung udara cobalah bakar lidi sampai membara kemudian
masukkan bara tersebut ke tabung reaksi yang sebelumya ditutup jari tangan terlebih
dahulu.
5. Catat hasil percobaan pada tabel pengamatan!



SMA Negeri 1 Depok
Jl. Nusantara Raya 317
F-SMAN1DP-KR-05-02

E. HASIL PENGAMATAN

No. Tabung Perlakuan Banyak Buih Nyala api
1. Tabung 1 + 5 tetes H
2
O
2

2. Tabung 2 Dipanaskan, + 5 tetes H
2
O
2

3. Tabung 3 +10 tetes HCl kemudian + 5
tetes H
2
O
2


4. Tabung 4 +10 tetes NaOH, kemudian
+5 tetes H
2
O
2


5. Tabung 5 + 5 tetes H
2
O
2

6. Tabung 6 Dipanaskan, + 5 tetes H
2
O
2

7. Tabung 7 +10 tetes HCl kemudian + 5
tetes H
2
O
2


8. Tabung 8 +10 tetes NaOH, kemudian
+5 tetes H
2
O
2



F. PERTANYAAN
1. Mengapa pada ekstrak hati yang diberi H
2
O
2
muncul gelembung udara?
................................................................................................................................................
................................................................................................................................................
2. Mengapa di dalam jaringan terdapat H
2
O
2
?
................................................................................................................................................
................................................................................................................................................
3. Apa pengaruh H
2
O
2
terhadap jaringan bila kadarnya berlebih?
................................................................................................................................................
................................................................................................................................................
4. Bagaimana cara tubuh menetralkan H
2
O
2
tersebut?
................................................................................................................................................
................................................................................................................................................
5. Dengan demikian apa fungsi enzim katalase? Tuliskan persamaan reaksi
kimianya!
................................................................................................................................................
................................................................................................................................................
6. Dimanakah enzim katalase terdapat?
................................................................................................................................................
................................................................................................................................................
7. Manakah yang lebih banyak enzim katalase yang ada di hati, atau bayam merah ,
apa buktinya?
................................................................................................................................................
................................................................................................................................................
8. Faktor-faktor apa saja yang mempengaruhi kerja enzim katalase?
................................................................................................................................................
................................................................................................................................................
9. Apa yang dimaksud dengan metabolisme?
................................................................................................................................................
................................................................................................................................................

G. KESIMPULAN
..........................................................................................................................................................
..........................................................................................................................................................
..........................................................................................................................................................
...................................................................................................................................................


....................................... 9................................................................................................. ............................................................ G........ 4............................................................................................................ 5.............. kemudian +5 tetes H2O2 + 5 tetes H2O2 Dipanaskan........................ Manakah yang lebih banyak enzim katalase yang ada di hati............... 7.................. 6............... Tabung Tabung 1 Tabung 2 Tabung 3 Tabung 4 Tabung 5 Tabung 6 Tabung 7 Tabung 8 Perlakuan + 5 tetes H2O2 Dipanaskan... 4................ + 5 tetes H2O2 +10 tetes HCl kemudian + 5 tetes H2O2 +10 tetes NaOH.....................E.......................................................................................... kemudian +5 tetes H2O2 Banyak Buih Nyala api F....................................................................... 3................... ......................... 7...... ......................................................................................................................................................................... ............................. Dimanakah enzim katalase terdapat? .. .................. Faktor-faktor apa saja yang mempengaruhi kerja enzim katalase? ...................................... ...................................................................................................................................................................................................................................................................................................................................................... Apa pengaruh H2O2 terhadap jaringan bila kadarnya berlebih? ... Mengapa di dalam jaringan terdapat H2O2 ? .................................. ................................................................................................................................................................................................ HASIL PENGAMATAN No...................................................................................................... 2............................................ atau bayam merah ..... ....................................... KESIMPULAN ................................................................................................................................. ................... ................................................................. 6.................. .................... .... Dengan demikian apa fungsi enzim katalase? Tuliskan persamaan reaksi kimianya! ......... 2................................................. apa buktinya? ...................................... 5............................................................................................................................. 8....................................... ................................................................................................................. 1................................................................ 3........................................................................... PERTANYAAN 1......................................................................... Bagaimana cara tubuh menetralkan H2O2 tersebut? .............................................................................................. 8................................... Mengapa pada ekstrak hati yang diberi H2O2 muncul gelembung udara? ........................................................................................................................................................................................................................................................................................................................................................................................................................... Apa yang dimaksud dengan metabolisme? .................................................................................................................................................................................... + 5 tetes H2O2 +10 tetes HCl kemudian + 5 tetes H2O2 +10 tetes NaOH...

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