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Experiment No.

I Glasswares and Equipments used in Hematology

I.

Overview
Hematology is the study of the nature, function, and diseases of the

blood and of blood-forming organs. It includes the diagnosis, treatment, prognosis, and prevention of blood diseases. The laboratory work that goes into the study of blood is frequently performed by a medical technologist. Therefore in doing these entire things, one must be familiar with all the equipments, glasswares as well as reagents, their individual functions and their specific uses with each hematological test and procedures so that results will be precise and accurate.

II.

Objectives
At the end of the experiment, the students should be able to: 1. Differentiate glasswares and equipments used in various hematological procedures. 2. Describe the appearance of the different equipments. 3. Identify their uses and functions. 4. Illustrate the different equipments and glasswares.

III.

Procedure
Examine all glasswares and equipments to be able to identify and

illustrate them.

IV.

Illustration and Research Questions


An instrument used for colorimetric determination of the blood's hemoglobin.

A. Sahli Hemoglobin Pipette and Comparator Block

B. Serological Pipette - Temperature calibrated devices used to transfer a desired volume of solution from one container to another.

C. Blood Lancet/Automatic Lancet a. Blood lancet - is used to make punctures to obtain small blood specimens, are generally disposable, and are commonly used in the treatment of diabetes b. Automatic lancet used for sugar testing and allows fast response about the status of the bodys glucose level

D. Capillary tubes Heparinized tubes are uniformly coated with ammonium heparin to prevent coagulation. Ammonium heparin does not interfere with sodium determination. Heparinized tubes are color coded red and non-heparinized (plain) tubes are color coded blue Excellent for use in micro-hematocrit determinations

E. Tourniquet Constricting or compressing device used to control venous and arterial circulation to an extremity for a period of time. To make veins more prominent

F. Syringe A medical instrument used to inject fluids into the body or draw them from it.

G. Vacutainer system Used in multiple collection of blood

H. Butterfly Infusion set Small vein (butterfly) infusion sets are ideal for use on children or adults with smaller veins. Flexible wings with textured surface easily pinch together to assure a secure grip

I. Pasteur pipette Used to transfer small amount of blood to westergren and wintrobe tube.

J. Thoma WBC and RBC diluting a. Thoma RBC pipet - manual glass pipette which dilutes the blood
specimen 1:200

b. Thoma WBC pipet - manual glass pipette which dilutes the blood
specimen 1:20

K. Electronic water bath Used for electronic room temperature and water level control for heating for heating various solutions.

L. Westergren tube and rack Used in erythrocyte sedimentation rate

M. Wintrobe tube and rack Used in erythrocyte sedimentation rate and hematocrit determination

N. Wet house chamber Used in direct counting of platelets

O. Hemocytometer Used as counting chamber and for counting blood cells

P. Test tube EDTA Used to contain blood specimen It is an anticoagulant for blood samples for CBC/FBEs.

Q. Test tube rack Used to hold test tubes

R. Staining rack Used to hold the glass slides in staining blood smears

S. Glass slide and cover slips Used to determine components of blood under the microscope Used in preparation of blood smear Used to prepare the specimen that is to be viewed under the microscope

T. Microhematocrit centrifuge machine Used to separate blood components using capillary tubes

U. Serofuge machine Designed to handle clinical samples facilitating blood testing procedures. Used to separate blood components

V. Microhematocrit reader Used to measure packed red cell volume

W. Sphygmomanometer Used to determine fragility of capillaries

X. Binocular Microscope Used in viewing blood smears and to study blood components

Y. Timer Used to determine bleeding and clotting time

Z. Tally Counter Used in determining blood cell count under microscope

AA. -

Differential Tally Counter Used to determine the numbers of different blood cells under the microscope

BB. Unopette for counting WBCs, RBCs, & platelets

V.

Updates

BD has launched the Vacutainer Rapid Serum Tube, a blood collection device designed to help acute healthcare facilities to rapidly analyze blood serum for patient diagnosis. The device features the BD Hemogard safety-engineered closure, which enhances healthcare worker safety and aids compatibility with clinical analyzers utilizing front-end automation. 'Technologies such as the BD Vacutainer Rapid Serum Tube can help reduce the time it takes to get answers from blood tests and have a significant impact on patient care and hospital productivity,' said Ana Stankovic, managing

director and vice-president of medical and scientific affairs and clinical operations for pre-analytical systems at BD Diagnostics. With a five-minute clotting time and as little as three minutes of centrifugation (at 4,000g), the Vacutainer Rapid Serum Tube can offer savings of up to 32 minutes of laboratory time, according to the company. Standard serum separator tubes require a 30-minute clot time followed by 10 minutes of centrifugation before clinical laboratory professionals can begin analyzing the sample and getting test results. 'By taking essentially one sixth of the time of standard serum tubes to clot, the BD Vacutainer Rapid Serum Tube is poised to help facilities significantly improve patient throughput, especially in places such as

emergency rooms where decisions to admit or treat patients are often based on blood-test results,' said Stankovic. The 5ml-draw, 13 x 100ml plastic sterile blood collection tube combines a thrombin-based additive with gel technology, which creates a high-quality serum sample by minimizing fibrin formation and hemolysis. Hemolysis and clotted specimens are two common examples of suboptimal quality, both of which can lead to laboratory errors. Studies conducted by BD show a 59 per cent reduction of haemolysis and fibrin strand formation in the Vacutainer Rapid Serum Tube versus the serum control tube. This observed sample quality improvement may help to lower the number of repeat collections by phlebotomy, which in turn can increase the productivity of the laboratory. Laboratory Talk Editorial Team (Aug. 11, 2011)

VI.
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References:
http://abacus.bates.edu/~ganderso/biology/resources/serological_pipet. html

y y

http://www.medexinternational.com/htm/bctubes.htm http://www.gmi-inc.com/Becton-Dickinson-Sero-Fuge-2001Centrifuge.html

y y

http://www.answers.com/topic/syringe#ixzz1fF4JdLij http://www.ctcd.edu/mlt/mbyrd/mlax1415/documents/HemLab6A6Man ualRBManualRBC_000.pdf

http://www.laboratorytalk.com/news/bdb/bdb142.html