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PRAKTIKUM MIKROBIOLOGI INDUSTRI

TEKNIK PEMINDAHAN BIAKAN SECARA ASEPTIK DAN ISOLASI


BAKTERI

Oleh :
Kelompok : 4
1. Gladys Sumargo

/ 5203010010

2. Phanvinson

/ 5203009017

Nama Asisten : Ailen Tanjaya, ST.


Hari / Tanggal Praktikum : Jumat / 9 Maret 2012

LABORATORIUM TEKNOLOGI BIOPROSES


JURUSAN TEKNIK KIMIA
FAKULTAS TEKNIK
UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA
2012

LEMBAR PENGESAHAN
Hari / tanggal percobaan : Jumat / 9 Maret 2012
Judul percobaan

: Teknik Pemindahan Biakan Secara Aseptik dan Isolasi Bakteri

Kelompok

:4

Asisten

Tanggal
pemeriksaan

: Ailen Tanjaya, ST.

Catatan dari asisten

1.

2.

3.

4.

5.

ii

Tanggal

Tanda tangan

pengembalian

Asisten

DAFTAR ISI
Halaman Judul .......................................................................................................... i
Lembar Pengesahan ................................................................................................. ii
Daftar Isi ................................................................................................................... iii
Daftar Gambar .......................................................................................................... iv
Bab I

Bab II

Pendahuluan ............................................................................................... 1
I.1.

Tujuan Percobaan ............................................................................ 1

I.2.

Tinjauan Pustaka .............................................................................. 1

Metodologi Percobaan ............................................................................... 10


II.1. Alat ................................................................................................... 10
II.2. Bahan ............................................................................................... 10
II.3. Prosedur Percobaan ......................................................................... 11
II.4. Pembuatan Larutan NA.................................................................... 18

Bab III Hasil Percobaan dan Pembahasan ............................................................. 19


III.1. Hasil Percobaan ............................................................................... 19
III.2. Pembahasan ..................................................................................... 21
Bab IV Kesimpulan ................................................................................................ 25
Daftar Pustaka .......................................................................................................... 26
Lampiran Laporan Sementara

iii

DAFTAR GAMBAR
Gambar
I.1
Goresan
T
.......................................................................................................................................................
2
Gambar
I.2
Goresan
Kuadran
.......................................................................................................................................................
2
Gambar
I.3
Goresan
Radian
.......................................................................................................................................................
3
Gambar
I.4
Goresan
Sinambung
.......................................................................................................................................................
3
Gambar

I.5
Teknik
Pengenceran
...................................................................................................................................
7

Gambar

II.1
Pembagian
Sektor
dalam
Metode
Cawan
Gores
...................................................................................................................................
14

Gambar

II.2 Cara Melakukan Goresan pada Agar di Cawan Petri


...................................................................................................................................
15

Gambar

II.3
Sektor
0
dan
Sektor
1
yang
Telah
digores
...................................................................................................................................
16

Gambar

II.4
Sektor 0, Sektor 1, dan sector 2 yang Telah digores
...................................................................................................................................
16

Gambar

II.5
Seluruh
Sektor
Telah
digores
...................................................................................................................................
16

Gambar

II.6
Langkah-Langkah
dalam
Metode
Cawan
Tuang
...................................................................................................................................
17

iv