1. Colligative properties are physical properties of a solution that change with variations in the number of dissolved particles.

A) True B) False Points Earned: 1.0/1.0 *Correct Answer(s):* True 2. What are the two main advantages of fluorometry over conventional spectrophotometry? A) smaller sample size and shorter reaction time B) increased sensitivity and specificity C) not affected by temperature or pH fluctuations D) lower cost of reagents and increased sensitivity Points Earned: 1.0/1.0 *Correct Answer(s):* B 3. What type of lamp is used for Atomic Absorption? A) helium-xenon B) cathode ray C) LASER D) hollow cathode lamp of specific element Points Earned: 1.0/1.0 *Correct Answer(s):* D 4. When measuring turbidity, the amount of light blocked by a suspension depends only upon its concentration. A) True B) False *Feedback: *correct -turbidity measurements are based on both the concentration of particles AND the SIZE of the particles. Points Earned: 1.0/1.0 *Correct Answer(s):* False 5. In Atomic Absorption, the lamp must be a specific type that matches the element you are trying to measure. A) True B) False Points Earned: 1.0/1.0 *Correct Answer(s):* True 6. The four colligative properties of a solution will all decrease when the number of dissolved particles increases. A) True B) False *Feedback: *freezing point and vapor pressure both decrease with increased dissolved particles, BUT boiling point and osmotic pressure both increase with increased dissolved particles. Points Earned: 1.0/1.0 *Correct Answer(s):* False

7. Which of these is true of fluorescence? A) it produces atoms in their ground state using a hollow cathode lamp B) it is commonly used to measure lead and lithium levels in the clinical lab C) detectors are placed at 15 and 30 degrees to detect forward light scatter D) it is about one thousand times more sensitive than a regular spectrophotometer method and varies with temperature and pH Points Earned: 1.0/1.0 *Correct Answer(s):* D 8. Which is true of chemiluminescence? A) rapid reaction time B) very sensitive C) no excitation radiation is required D) uses luminol, acridinium esters, and/or dioxetanes E) all of these are true Points Earned: 1.0/1.0 *Correct Answer(s):* E 9. What type of testing is usually performed using Atomic Absorption? A) hematology cell line identifications B) immunology titers C) trace element levels D) vitamin levels Points Earned: 1.0/1.0 *Correct Answer(s):* C 10. A super-cooled solution ... A) is a liquid several degrees below the typical freezing point B) is formed in the osmometer, and then begins to freeze after vigorous agitation C) will freeze at a higher temperature when it contains more impurities D) all of the above E) only choice 1 and 2 are correct Points Earned: 1.0/1.0 *Correct Answer(s):* E