Spring 2009
Mr. Vermillion
1. What was the prevailing method of explaining the inheritance of traits before
Mendel’s work with pea plants?
2. Garden pea plants are naturally __________-fertilizing, but Mendel took steps to
__________-fertilize them for his experiments.
3. __________ are units of information about specific traits; they are passed from
parents to offspring. What is the general term applied to the location of a gene on
a chromosome?
4. Define allele; how many types of alleles are present in the genotypes Tt? tt? TT?
6. When two alleles of a pair are identical, it is a __________ condition; if the two
alleles are different, this is a __________ condition.
7. Distinguish a dominant allele from a recessive allele.
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11. Be able to use the Punnett-square method of solving genetics problems.
13. Mendel's theory of __________ states that during meiosis, the two genes of each
pair separate from each other and end up in different gametes.
14. Describe the testcross.
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19. Explain why sickle-cell anemia is a good example of pleiotropy.
20. Gene interaction involving two alleles of a gene that mask expression of another
gene's alleles is called __________.
21. List possible explanations for less predictable trait variations that are observed.
22. List two human traits that are explained by continuous variation.
23. Himalayan rabbits and garden hydrangeas are good examples of environmental
effects on __________ expression.
24. List two human traits that are explained by continuous variation.
25. Himalayan rabbits and garden hydrangeas are good examples of environmental
effects on __________ expression.
1. The first abnormal chromosome tied to cancer was named the __________
chromosome.
2. A __________ is a preparation of metaphase chromosomes based on their
defining features.
3. The units of information about heritable traits are known as __________.
4. Diploid (2n) cells have pairs of __________ chromosomes.
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5. __________ are different molecular forms of the same gene that are possible at a
given locus; a __________-type allele is the most common form of a gene.
6. State the circumstances required for crossing over and describe the results.
__________ __________ refers to the random alignment of each pair of
homologous chromosomes at metaphase I of meiosis.
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8. Name and describe the sex chromosomes in human males and females.
9. Human X and Y chromosomes fall into the more general category of __________
chromosomes; all other chromosomes in an individual’s cells are the same in both
sexes and are called __________.
10. Define karyotype; briefly describe its preparation and value.
11. Explain meiotic segregation of sex chromosomes to gametes and the subsequent
random fertilization that determines sex in many organisms.
13. In whose laboratory was sex linkage in fruit flies discovered? Approximately
when?
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14. State the relationship between crossover frequency and the location of genes on a
chromosome.
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23. When gametes or cells of an affected individual end up with one extra or one less
than the parental number of chromosomes, it is known as __________; relate this
concept to monosomy and trisomy.
24. Having three or more complete sets of chromosomes is called __________.
25. __________ is the failure of the chromosomes to separate in either meiosis or
mitosis.
26. Trisomy 21 is known as __________ syndrome; Turner syndrome has the
chromosome constitution, __________; XXY chromosome constitution is
__________ syndrome; taller than average males with sometimes mild mental
impairment have the __________ condition.
27. Explain what is meant by double-blind studies.
28. List some benefits of genetic screening and genetic counseling to society.
29. Explain the procedures used in three types of prenatal diagnosis: amniocentesis,
chorionic villi analysis, and fetoscopy; compare the risks.
30. Discuss some of the ethical considerations that might be associated with a
decision of induced abortion.
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1. Before 1952, __________ molecules and __________ molecules were suspected
of housing the genetic code.
2. The two scientists who assembled the clues to DNA structure and produced the
first model were __________ and __________.
3. Summarize the research carried out by Miescher, Griffith, Avery and colleagues,
and Hershey and Chase; state the specific advances made by each in the
understanding of genetics.
4. Viruses called __________ were used in early research efforts to discover the
genetic material.
5. Summarize the specific research that demonstrated that DNA, not protein,
governed inheritance.
6. Draw the basic shape of a deoxyribose molecule and show how a phosphate group
is joined to it when forming a nucleotide.
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8. DNA is composed of double-ring nucleotides known as __________ and single-
ring nucleotides known as _________; the two purines are __________ and
__________, while the two pyrimidines in DNA are __________ and
__________.
9. Assume that the two parent strands of DNA have been separated and that the base
sequence on one parent strand is A-T-T-C-G-C; the base sequence that will
complement that parent strand is __________.
10. List the pieces of information about DNA structure that Rosalind Franklin
discovered through her x-ray diffraction research.
11. Explain what is meant by the pairing of nitrogen-containing bases (base pairing),
and explain the mechanism that causes bases of one DNA strand to join with
bases of the other strand.
14. During DNA replication, enzymes called DNA __________ assemble new DNA
strands.
15. Distinguish between continuous strand assembly and discontinuous strand
assembly.
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Review Questions - Chapter 14 “DNA and Protein Synthesis”
1. State how RNA differs from DNA in structure and function, and indicate what
features RNA has in common with DNA.
4. What RNA code would be formed from the following DNA code: TAC-CTC-
GTT-CCC-GAA?
8. Scrutinize Figure 13.7 in the text and decide whether the genetic code in this
instance applies to DNA, mRNA, or tRNA
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9. Explain how the DNA message TAC-CTC-GTT-CCC-GAA would be used to
code for a segment of protein, and state what its amino acid sequence would be.
10. Describe how the three types of RNA participate in the process of translation.
11. Cite an example of a change in one DNA base pair that has profound effects on
the human phenotype.
12. Briefly describe the spontaneous DNA mutations known as base-pair substitution,
frameshift mutation, and transposable element change.
13. List some of the environmental agents, or __________, that can cause mutations.
14. Using a diagram, summarize the steps involved in the transformation of genetic
messages into proteins. (see Figures in your text)
1. Name the primary male reproductive organs; list secondary sexual traits
determined by these organs. [p.656]
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2. Follow the path of a mature sperm from the seminiferous tubules to the urethral
exit. List every structure encountered along the path, and state the contribution to
the nurturing of the sperm. [pp.656-657]
4. Name the four hormones that directly or indirectly control male reproductive
function. Diagram the negative feedback mechanisms that link the hypothalamus,
anterior pituitary, and testes in controlling gonadal function. [pp.658-659]
5. Diagram the structure of a sperm, label its components, and state the function of
each. [p.659]
6. Trace the path of a sperm from the urethral exit to the place where fertilization
normally occurs. Mention in correct sequence all major structures of the female
reproductive tract that are passed along the way, and state the principal function of
each structure. [pp.660-661]
7. Distinguish the follicular phase of the menstrual cycle from the luteal phase, and
explain how the two cycles are synchronized by hormones from the anterior
pituitary, hypothalamus, and ovaries. [pp.660-661]
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8. State which hormonal event brings about ovulation and which other hormonal
events bring about the onset and finish of menstruation. [pp.662-663]
9. List the physiological factors that bring about erection of the penis during sexual
stimulation and the factors that bring about ejaculation. [p.665}
10. List the similar events that occur in both male and female orgasm. [p.665]
11. Distinguish between the embryonic period and the fetal period. [p.666]
12. Describe the events that occur during the first month of human development;
include the developed structures of the blastocyst as seen at about 15 days.
[pp.666-667]
14. List and describe the four extraembryonic membranes and their functions. [p.667]
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15. Characterize the human developmental events occurring from day 15 to day 25.
[p.668]
18. The embryonic period of human development ends after the __________ week is
over; the embryo is now clearly a human __________. [p.670]
19. Explain why the mother must be particularly careful of her diet, health habits, and
life-style during the first trimester after fertilization (especially during the first six
weeks). [pp.672-673]
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24. Cite the distinguishing characteristics of Werner’s syndrome. [p.675]
26. Identify the three most effective birth control methods used in the United States
and the four least effective birth control methods. [p.677]
27. For each STD described in Focus on Health, know the causative organism and the
symptoms of the disease. [pp.678-679]
28. State the physiological circumstances that would prompt a couple to try in vitro
fertilization.
Answer all of the multiple choice questions at the end of each of these
chapters. Check your work with the key.
Example Problem
In summer squash, white fruit color (W) is dominant over yellow fruit color (w). If a
squash plant homozygous for white is crossed with a plant homozygous for yellow, what
will the phenotypic and genotypic ratios be for:
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a. the F1 generation? b. the F2 generation?
c. What will the phenotypic and genotypic ratios of the offspring be if you perform a
testcross with the F1 generation?
Solution
1. Write down the cross in terms of the parental (P1) genotypes and phenotypes:
WW x ww white x yellow
2. Determine the P1 gametes, place them in a Punnett Square and fill in the resulting
genotypes:
P1 gametes W and w
W W
w Ww Ww
Ww Ww
w
5. Determine the F1 gametes, place them in a Punnett Square and fill in the resulting
genotypes:
W w
WW Ww W
Ww ww
w
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Genotypic ratios: 1:2:1
Case (2)
Genotypic ratios: Case (1) 1:1 (Ww and ww) and case (2) 100% (Ww)
Phenotypic ratios: 1:1 (White: yellow and 100% (Ww will be yellow)
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Problem 1: The Monohybrid Cross
In pea plants, spherical seeds (S) are dominant to
dented seeds (s). In a genetic cross of two plants that
are heterozygous for the seed shape trait, what
fraction of the offspring should have spherical
seeds?
A. None
B. 1/4
C. 1/2
D. 3/4
E. All
A genetic cross between two F1-hybrid pea plants for spherical seeds will yield what
percent spherical-seeded plants in the F2 generation? (spherical is dominant over dented)
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A. 100%
B. 75%
C. 50%
D. 25%
E. 0%
A genetic cross between two F1-hybrid pea plants having yellow seeds will yield what
percent green-seeded plants in the F2 generation? Yellow seeds are dominant to green.
A. 0%
B. 25%
C. 50%
D. 75%
E. 100%
When true-breeding tall stem pea plants are crossed with true-breeding short stem pea
plants, all of the _________ plants, and 3/4 of the __________ plants had tall stems.
Therefore, tall stems are dominant.
A. F1, F2.
B. G1, G2.
C. parental, F2.
D. F2, parental.
E. P1, P2
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To identify the genotype of yellow-seeded pea plants as
either homozygous dominant (YY) or heterozygous (Yy),
you could do a test cross with plants of genotype
_______.
A. y
B. Y
C. yy
D. YY
E. Yy
A test cross is used to determine if the genotype of a plant with the dominant phenotype
is homozygous or heterozygous. If the unknown is homozygous, all of the offspring of
the test cross have the __________ phenotype. If the unknown is heterozygous, half of
the offspring will have the __________ phenotype.
B. recessive, dominant
C. dominant, epistatic
D. co-dominant, complimentary
E. dominant, recessive
In Mendel's experiments, if the gene for tall (T) plants was incompletely dominant over
the gene for short (t) plants, what would be the result of crossing two Tt plants?
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E. All the offspring would be intermediate.
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Problem 1: Predicting combinations of alleles in gametes of plants heterozygous for
two traits.
A pea plant is heterozygous for both seed shape and seed color. S is the allele for the
dominant, spherical shape characteristic; s is the allele for the recessive, dented shape
characteristic. Y is the allele for the dominant, yellow color characteristic; y is the allele
for the recessive, green color characteristic. What will be the distribution of these two
alleles in this plant's gametes?
A. 50% of gametes are Sy; 50% of gametes are sY
B. 25% of gametes are SY; 25% of gametes are Sy;
25% of gametes are sY; 25% of gametes are sy.
C. 50% of gametes are sy; 50% of gametes are SY
D. 100% of the gametes are SsYy
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Which of the following genotypes would you not expect to find among the offspring of a
SsYy x ssyy test cross:
A. ssyy
B. SsYy
C. Ssyy
D. ssYy
E. SsYY
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If Mendel's crosses between tall, spherical-seeded plants and short, dented-seeded plants
had produced many more than 1/16 short, dented-seeded plants in the F2 generation, he
might have concluded that:
A. the dented seed and short traits are unlinked.
B. he would not have concluded any of the above.
C. all traits in peas assort independently of each other.
D. the spherical seed and tall traits are linked.
E. all traits in peas are linked.
Source
*Go to this site for a complete tutorial for these problems:
http://www.biology.arizona.edu/mendelian_genetics/problem_sets/dihybrid_cross/di
hybrid_cross.html
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Population Genetics and the Hardy-Weinberg Law
A population is defined as an interbreeding group. The fact that the frequencies of the
alleles may change from one generation to the next is known as evolution. Only
populations can evolve. Individual organisms within a population can not.
The environment will test the "fitness" of a combination of genes. Those that are not fit
will be removed from the gene pool.
Gene pool - the sum total of all the genes possesed by all the eligible breeding
individuals in a population. The gene pool links one generation to the next.
A population is not the same as a gene pool. Only those members of population capable
of reproducing are in the gene pool. Immature juveniles and those unable to mate are not
in the gene pool.
Gene frequency refers to the frequency of a given allele in a population. If the dominant
allele occurs 90% (.9) of the time then the recessive allele must occur 10% (.1) of the
time. Thus it follows that 81% of the population is homozygous dominant (AA), 18% is
heterozygous (Aa), and 1% is homozygous recessive (aa). See the following tables.
Sperm
0.9 A 0.1 a
Egg 0.9 A 0.81 AA 0.09 Aa
0.1 a 0.09 Aa 0.01 aa
If the gene frequencies were equal 50% (0.5) A and 50% (0.5) a, then the following
would be true: 25% of the population is homozygous dominant (AA), 50% is
heterozygous (Aa), and 25% is homozygous recessive (aa).
Sperm
0.5 A 0.5 a
Egg 0.5 A 0.25 AA 0.25 Aa
0.5 a 0.25 Aa 0.25 aa
Hardy-Weinberg Law
In 1908, two mathematicians, Godfrey Hardy and Wilhelm Weinberg, wrote an equation
to describe gene frequency changes in a population. It became known as the Hardy-
Weinberg Law. The law states that under conditions of equilibrium both the gene
frequencies and genotypic ratios remain constant from generation to generation in
sexually reproducing populations. For equilibrium to occur the following conditions must
be met:
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• The population must be larger than 10,000 individuals with total random mating;
therewise, chance alone can alter the gene frequencies. See genetic drift.
• No gene mutations can occur in the population.
• No genes may enter of leave the population. There is no immigration (entrance of
organisms) or emigration (exit of organisms).
• There is no natural selection. There is no pressure to "select" a mate (due to
phenotype).
• In addition, it assumes that each member of the population mates and produces an
equal number of offspring.
Because no population can meet all of the above conditions of equilibrium the gene
frequencies of a population will change. This is known as evolution.
Genetic drift is the result of random fluctuations in the allele frequencies due to a small
population.
The Hardy-Weinberg problems can be solved using the following equations. The
dominant allele, A is represented by the letter "p" and the recessive allele, a, by the letter
"a". Therefore: AA = pp or p2 , Aa = pq, and aa = qq or q2
Equation 1 p + q = 1 or 100%
Equation 2 (p + q)2 = p2 + 2pq + q2 =
(binomial) 100%
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Instructions: Using a calculator, solve the following problems. Rounding off to the
nearest whole number or 1/10 when solving for p or q. If you do not have a calculator, set
the problem up without finding the square root. Use the following equations: ..............
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______________________
4d. How many people out of 8000 will be homozygous
recessive? 4d
______________________
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Monohybrid Cross Problems
Example Problem
In summer squash, white fruit color (W) is dominant over yellow fruit color (w). If a
squash plant homozygous for white is crossed with a plant homozygous for yellow, what
will the phenotypic and genotypic ratios be for:
Solution
1. Write down the cross in terms of the parental (P1) genotypes and phenotypes:
WW X ww
w
W Ww
All F1 progeny will be heterozygous (Ww) and will therefore have white fruit color.
5. Determine the F1 gametes, place them in a Punnett Square and fill in the resulting
genotypes:
Ww X Ww
W w
W WW Ww
w Ww ww
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6. Determine the genotypic and phenotypic ratios for the F2 generation:
Genotypic ratios: 1/4 will be homozygous dominant (WW), 1/2 will be heterozygous
(Ww) and 1/4 will be homozygous recessive (ww). This is a 1:2:1 genotypic ratio.
Phenotypic ratios: 3/4 will have white fruit color and 1/4 will have yellow fruit color.
This is a 3:1 phenotypic ratio.
w
W Ww
w ww
Genotypic ratios: 1/2 heterozygous (Ww) and 1/2 homozygous recessive(ww). This is a
0:1:1 genotypic ratio.
Phenotypic ratios: 1/2 will have white fruit color and 1/2 will have yellow fruit color.
This is a 1:1 phenotypic ratio.
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