Harare Institute of Technology

Department of Chemical and Process Systems Engineering

Course Outline Course Bioprocess Engineering Principles Code ECP 424 Lecturer Ms. M Manyuchi and Mr.LKadzungura Office W10 Credit 2 Contact hrs 72 hrs 1.0 Course Aim and Objectives Biochemical engineering is a branch of chemical engineering that mainly deals with the design and construction of unit processes that involve biological organisms or molecules and equipment such as bioreactors. Its applications are used in the food, pharmaceutical, biotechnology and water treatment industries (www.wikipedia.com). The aim of the course is to teach students the fundamental understanding of biochemical reactors of different types and configuration, both steady- states.In addition, unsteady- state principles for homogeneous and heterogeneous reactors and lumped and distribute (tubular) systems. At the end of the course, the student must be able to:

Understand the terminology and concepts of the bioprocess industries Understand the biological organisms used in bioprocesses and their kinetics Understand the characteristics of biochemical kinetics and its relation to biochemical reactor design Develop stead-state and unsteady- state and control equation for different types of biochemical reactors Analysis of the characteristic of different types of bio- reactors Calculate oxygen transfer rate in aerobic bio-processes Understand the importance of sterilisation and containment in bioprocesses Understand downstream processing techniques applied in bioprocesses Period Week 1&2

2.0 Course Content Learning Points 1. Cells and Cell Kinetics Classification and composition of cells Monods equation Batch growth kinetics Product formation kinetics Nutrient limitations
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Continuous culture Quiz

2. Enzymes and Enzyme Kinetics Properties of enzymes Week 3&4 Types of enzymes Michaeli-Mentens equation Enzyme kinetics Lineweaver-Burke plot Eadie-Hofstee plot Enzyme inhibition Enzyme immobilisation Quiz 3. Bioreactors Design and Control Types of bioreactors Design of Bioreactors Stoichiometry and kinetics of bioreactors Ideal reactor operation (dynamics and control) Bioreactor stability Bioreactor system supplies Quiz 4. Oxygen Transfer in Bioprocesses Importance of oxygen transfer in biological systems Two film theory applications in bioprocesses Oxygen transfer limitations in bioprocesses Oxygen transfer measurement methods Quiz Week 7&8 Week 5&6

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5.Containment, Sterilization Kinetics Containment methods Sterilisation methods Sterilisation equipment

and

Sterilization

Week 9& 10

Advantages and disadvantages of containment and sterilisation Sterilisation kinetics Del factor Arrhenius plot Containment and containment design Quiz 6.Downstream Processes Identify need for downstream processes Downstream processes unit operations Downstream processes energy requirements Product recovery Product purification Quiz

Week 11 & 12

3.0 Course Teaching and Assessment The course will be taught through the following methods: Teaching Method Time Period Lectures 48 hrs Tutorials 12 hrs Practicals (Industrial visit, Cells and cell 12 hrs composition and Sterilization Kinetics) Assessment will be done as follows: Assessment Methods Intended number Assignments 3 Tests 3 Practicals 2 Final Exam 1 4.0 Course Policy Students should be aware of the following: Contribution 5% 10% 25% 60%

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1. Any student without theory coursework or practical coursework or both is not

allowed to sit for the final exam

2. Any student who attends less than 80% of lectures, tutorials and practical

classes is not qualified to enter into the final examination

3. Students should be punctual for classes, latecomers will not be allowed 4. All assignments and practical reports must be submitted on the due date

5.0 Text Books Required Text: 1. NIIR BOARD, Enzyme Biotechnology Handbook, Asian Pacific Business Press Inc, India 2. NIIR BOARD, Biotechnology Handbook, Asian Pacific Business Press Inc, India 3. J.E Bailely and D.F Ollis, 1986 Biochemical Engineering Fundamentals, 2nd edition McGraw Hill Book Co, New York 4. J A Asenjo and J.C Merchunk (ed), 1995 Bioreactor System Design (1995) Marcel Decker Incl, New York 5. J Nielsen et al, 2003 Bioreactor Engineering Principles, 2nd edition Plenun Publishers, New York 6. Levenspiel 2004 Chemical Reaction Engineering 3rd edition, John Wiley and Sons Pvt Ltd Singapore 7. Coulson and Richardson 2006 (1986) Volume 3 Chemical and Biochemical Reactors and Process Control, 3rd edition, Butterworth-Heinemann Supplementary References: R.H.Perry, Don.W.Green, J.O.Maloney; Perry`s Chemical Engineer`s Handbook; McGraw-Hill; 7th edition; 1997. Journal References

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