Sentrifuge 200 rpm

CARA KERJA

Baku PCT 50 mg

ad 50 ml
EtOH

Di Pipet 1 ml

10x10 cm

ad 10 ml

2l

4l

6l

8l

10l

4l

4l

4l

Elusi : heksan : etil

(1: 2)
Baca pada 254 nm

Pengencerean sampel
Sampel PCT~50 mg

50 mL etanol (1000 ppm)

(10 L x 1 g/L = 4 g)

Pengenceran Baku
Parasetamol ~ 50 mg

50 mL etanol (1000 ppm)

2 L, 4 L, 6 L, 8 L, 10 l :
(2 L x 1 g/L = 2 g)
(4 L x 1 g/L = 4 g)
(6 L x 1 g/L = 6 g)
(8 L x 1 g/L = 8 g)
(10 L x 1 g/L = 10 g)

CONTOH
1 Tabel Pengamatan
Fraksi

Rf

Konsentrasi

Tinggi

totolan g

puncak

Luas Area

Baku 1

0,62

60,26

4967,09

Baku 2

0,64

101,19

11927,38

Baku 3

0,65

122,05

15745,51

Baku 4

0,66

155,58

21928,36

Baku 5

0,66

166,46

25091,75

Asetosal 1

0,62

110,10

10709,10

Asetosal 2

0,62

75,75

7353,29

Asetosal 3

0,63

57,57

5719,38

30000
y = 5024.9x + 856.9
R = 0.9858

25000

Area

20000
15000
10000
5000
0
0

Kosentrasi

IV.2 Perhitungan
Pengencerean sampel
Sampel asetosal ~ 250 mg

50 mL etanol (5000 ppm)

1 mL

10 mL etanol (500 ppm)

4 L
(4 L x 0,5 g/L = 2 g)

Pengenceran Baku
asetosal ~ 250 mg

50 mL etanol (5000 ppm)

1 mL

10 mL etanol (500 ppm)

4 L
(4 L x 0,5 g/L = 2 g)

Perhitungan Kadar
y = 856,928 + 2512,515x

Asetosal 1
10709,10 = 856,928 + 2512,515x
X = 3,92 g

%K

%K

= 196%

x 100%

Asetosal 2
7353,29 = 856,928 + 2512,515x
X = 2,5856 g

%K

x 100%

%K

= 129,28%

Asetosal 3
5719,38 = 856,928 + 2512,515x
X = 1,9353 g
%K

x 100%

%K

= 97,675%