Code: 9A23402

B.Tech II Year II Semester (R09) Regular & Supplementary Examinations, April/May 2013 ANALYTICAL METHODS IN BIOTECHNOLOGY (Biotechnology) Time: 3 hours Max. Marks: 70 Answer any FIVE questions All questions carry equal marks ***** 1 2 (a) (b) 3 (a) (b) 4 5 (a) (b) 6 7 8 Discuss in detail about electromagnetic spectrum. Write about: Precision and accuracy. Confidence limits. Write an account of: Principle of bright field microscopy. Flow cytometry. Write about Beer-Lamberts law and its deviations. Write notes on: X-ray diffraction. Circular dichromism. Explain in detail the principles and working of ultracentrifuge. Write the principles and applications of HPLC. Write in detail about types of radioactivity and applications of radioisotopes. *****

Code: 9A23402

B.Tech II Year II Semester (R09) Regular & Supplementary Examinations, April/May 2013 ANALYTICAL METHODS IN BIOTECHNOLOGY (Biotechnology) Time: 3 hours Max. Marks: 70 Answer any FIVE questions All questions carry equal marks ***** 1 2 (a) (b) 3 Explain the interaction between electromagnetic radiation and matter. Write notes on: Types of errors. Confidence limits. Write the principle and working of scanning electron microscopy add a note on its applications. Write the principle and working of UV-viable spectrophotometer. Write notes on: ORD. NMR. Write in detail about the technique involved in separation of proteins by electrophoresis. Write about the working principle and applications of GC. Write notes on: pH sensors. GM counters. *****

4 5 (a) (b) 6 7 8 (a) (b)

Code: 9A23402

B.Tech II Year II Semester (R09) Regular & Supplementary Examinations, April/May 2013 ANALYTICAL METHODS IN BIOTECHNOLOGY (Biotechnology) Time: 3 hours Max. Marks: 70 Answer any FIVE questions All questions carry equal marks ***** 1 (a) (b) 2 3 (a) (b) 4 5 (a) (b) 6 (a) (b) 7 8 Write notes on: Jablonski diagrams. Types of molecular energies. Explain the methods of expressing accuracy and precision. Write notes on: Confocal microscopy. TEM. Discuss principles and applications of atomic absorption spectroscopy. Discuss about: X-ray diffraction. Sedimentation co-efficient. Write the principle involved in: TLC. GPC. Write an account of online monitoring of dissolved oxygen and temperature. Explain about types of radioactive rays and their applications. *****

Code: 9A23402

B.Tech II Year II Semester (R09) Regular & Supplementary Examinations, April/May 2013 ANALYTICAL METHODS IN BIOTECHNOLOGY (Biotechnology) Time: 3 hours Max. Marks: 70 Answer any FIVE questions All questions carry equal marks ***** 1 (a) (b) 2 (a) (b) 3 4 (a) (b) 5 (a) (b) 6 7 (a) (b) 8 Write notes on: Electromagnetic spectrum. Types of molecular energies. Write in detail about: Types of errors. Sensitivity and detection limits for instruments. Write the principle and working of flow cytometry. Add notes on its applications. Discuss the following: X-ray diffraction. 2D gels. Explain the principles of: GC. HPLC. Explain the working and application of spectrofluorimeter. Write notes on: Online monitoring of agitation sensors. Online monitoring of dissolved oxygen. Write about the applications of radioisotopes to human welfare. *****