Code: 9A23603

B.Tech III Year II Semester (R09) Regular & Supplementary Examinations, April/May 2013 TRANSPORT PHENOMENA IN BIOPROCESS (Biotechnology) Time: 3 hours Max. Marks: 70 Answer any FIVE questions All questions carry equal marks ***** 1 2 3 Define Newtons law of viscosity. Write in detail about viscosity measurement. Derive the equation for flow through a circular tube in shell momentum balances. Two large flat porous horizontal plates are separated by a relatively small distance L. The upper plate at y = L is at a temperature and the lower plate at is to be maintained at a lower temperature . To reduce the amount of heat that must be removed from the lower plate, a coolant gas is blow upward through both the plates at steady state. Develop an expression for the temperature distribution using the equation of energy. Write short notes on: (a) Interaction between cells and turbulent eddies. (b) Power requirements for mixing in gassed fluids. What are the various mechanisms of heat transfer? Write the analogy between heat and momentum transfer in detail. Write in detail about: (a) Forced convection. (b) Derive the shell energy balance for heat conduction with a nuclear heat source. Write in detail about theory of diffusion and enumerate on estimation of diffusion coefficients for gases. What are the various physical and chemical factors influencing the oxygen transfer in fermenters?

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Code: 9A23603

B.Tech III Year II Semester (R09) Regular & Supplementary Examinations, April/May 2013 TRANSPORT PHENOMENA IN BIOPROCESS (Biotechnology) Time: 3 hours Max. Marks: 70 Answer any FIVE questions All questions carry equal marks ***** 1 (a) (b) 2 Write short note on: Coaxial cylinder rotary viscometer Impeller viscometer An incompressible fluid is flowing in steady state in the annular region between two coaxial circular cylinders of radii and R. Using the equations of change, obtain the equation for the determination of the following: Velocity gradient Maximum velocity Average velocity Derive equation of mechanical energy change for an isothermal system. Explain the mechanism of mixing. Write about the power requirements of mixing in ungassed Newtonian fluids. What are the various mechanisms of heat transfer and describe about the measurement of thermal conductivity. Write short notes on forced convection and derive the shell energy balance for heat conduction with chemical heat source. Explain in detail about diffusion with a homogeneous chemical reaction. Write in detail about oxygen uptake in cell cultures. What are the limitations of steady state and dynamic methods in experimental determination of

(a) (b) (c) 3 4

7 8

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Code: 9A23603

B.Tech III Year II Semester (R09) Regular & Supplementary Examinations, April/May 2013 TRANSPORT PHENOMENA IN BIOPROCESS (Biotechnology) Time: 3 hours Max. Marks: 70 Answer any FIVE questions All questions carry equal marks ***** 1 What are the uses of viscometers with fermentation broths? Explain the importance of osmotic pressure, product concentrations and cell concentration on broth viscosity. A metal plate, which was immersed in a liquid trough, is lifted vertically upward having an angle of inclination with the horizontal axis of the trough. The liquid film on the plate flows in laminar flow and its thickness is x. The dimensions of the plate are length = L, width = W. Derive an expression for the velocity distribution and volumetric flow rate. A fermentation broth with viscosity s and density is agitated in a baffled tank using a Rushton turbine with a diameter and stirrer speed Estimate the mixing time. Describe the power requirements of mixing in ungassed Newtonian fluids in turbulent region. Define Fouriers law of heat conduction. Derive the equation for steady state conduction of heat through a flat wall. Explain in detail about free convection and derive the shell energy balance for heat conduction with a viscous heat source. Derive the diffusion of liquid through a stagnant gas film. Derive equation of motion for an isothermal system. Explain in detail about oxygen balance method and dynamic method for measurement of

3 (a)

(b) 4

6 7 8

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Code: 9A23603

B.Tech III Year II Semester (R09) Regular & Supplementary Examinations, April/May 2013 TRANSPORT PHENOMENA IN BIOPROCESS (Biotechnology) Time: 3 hours Max. Marks: 70 Answer any FIVE questions All questions carry equal marks ***** 1 Explain in detail about rheological properties of fermentation broths and factors affecting broth viscosity. Derive the equation of continuity considering cylindrical co-ordinates. Reduce the equation for incompressible fluids. Derive the equation for flow of two adjacent immiscible fluids. A fermentation broth with viscosity pa.s and density is agitated in a baffled tank using a marine propeller in diameter. Calculate power required . Assume laminar flow, turbulent flow; for a stirrer speed of for turbulent flow 0.35 and for laminar flow 40. Describe the mechanism of mixing. Derive the equation for steady state conduction of heat through a flat wall and write the analogy between heat and momentum transfer in detail. Write in detail about free convection and derive the shell energy balance for heat conduction with an electrical heat source. Write short notes on estimation of diffusion coefficient in: Colloidal suspensions. Polymers. Write in detail about steps involved in oxygen transfer from gas bubble to cell culture.

2 (a) (b) 3 4 (a)

(b) 5

7 (a) (b) 8

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