Fibroblast Cell Culture Systems Fibroblast Cell Culture Medium Fibroblast Cell Culture Companion Products and Supplements

Human Primary Cell Culture Systems

Fibroblast Cell Culture Systems

Fibroblasts were the first adherent cell types to be successfully cultured in vitro. In mixed primary cultures fibroblast-like cells typically overgrow other adherent cell types. Fibroblast cells were frequently developed as host cells for the production of therapeutically useful viruses. Even today these cells are widely used for this purpose. Many classical media (see web-pages in our "Classical Media" section) were developed and used to culture fibroblasts. Some examples of these are; CMRC 1066, GMEM, Ham's F-10, L-15, and Medium 199. These media all required the addition of serum. In the 70s and 80s, Richard Ham and his colleagues developed MCDB 104, MCDB 105 and MCDB 110 as low protein serum-free media for growing fibroblast-like cells such as WI-38, MRC-5 and IMR-90 cells. These media were actually supplemented with dialyzed FBS, which was referred to as fetal bovine serum protein (FBSP).

Fibroblast Cell Culture Medium

In addition to all of the "classical media" referenced above, Sigma offers the fibroblast growth kit, FMK-2 which is composed of basal medium F 9790 and medium supplement F 9915. This is the most advanced medium formulation for fibroblast cell culture that we offer. Once formulated, the complete medium contains a very small amount of FBS. <> Product # F 9790 Product Name Fibroblast Basal Medium Description Application back to top

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Liquid Proprietary formulation, modified version of MCDB 105 Contains essential and non-essential amino acids, vitamins, trace minerals, organic and inorganic salts. Without antibiotics, antimycotics, hormones, growth factors, or proteins. Buffered with HEPES and sodium bicarbonate Sterile filtered Endotoxin tested Cell culture tested.

This basal medium is a component of our Fibroblast Proliferation Medium Kit (FMK-2). It is designed to be used in conjunction with Fibroblast Medium Supplement (F 9915), and it is not sold separately. The FMK-2 kit provides a complete medium for the proliferation of epidermal fibroblasts for research purposes.

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Fibroblast Cell Culture Companion Products and Supplements

The serum free culture of fibroblast cells is supported by a series of products offered by Sigma. The table below provides a partial list of Sigma products recommended for this purpose. Also, see the "Fibroblast Cell Culture Media" table above. This table may also be found by using the hyperlink at the top of this page. <> Product # F 9915 Product Name Fibroblast Medium Supplement (50x) Description Application back to top

Liquid Proprietary formulation Contains fetal bovine serum (2%), human recombinant bFGF heparin, human recombinant EGF, and hydrocortisone

This supplement is a component of our Fibroblast Proliferation Medium Kit (FMK2). It is designed to be used in conjunction with Fibroblast Basal Medium (E 9790), and it is not sold separately. The FMK-2 kit provides a complete


T 4299 Trypsin-EDTA Solution for Endothelial Cell Culture

Endotoxin tested Cell culture tested Liquid Contains 500 BAEE units porcine trypsin and 180 ug of EDTA74Na per mL in DPBS without calcium and magnesium Powder negative for porcine parvovirus by 9 CFR Sterile filtered Mycoplasma tested Cell culture tested Liquid Phosphate buffered saline solution containing (1x) 0.025% soybean trypsin inhibitor pH approximately 7.4 at room temperature Sterile filtered

fibroblasts for research purposes.

Useful for the dissociation of, or release of, adherent cells types, such as endothelial, epithelial, and fibroblast cells from support surfaces.

T 7659 Trypsin Inhibitor Defined

Use to inhibit the proteolytic action of Trypsin-EDTA (T 4299) after epithelial cells are released from a substrate or disaggregated. Chemically defined and free of animal derived components. The absence of animal derived components reduces the potential for contamination of cryopreserved cells with adventitious biological agents such as viruses.

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Human Primary Cell Culture Systems

Sigma is proud to offer a selection of media, many of which are serum-free, for the in vitro culture of human primary cells, such as keratinocytes, corneal epithelial cells, endothelial cells, fibroblasts, melanocytes and epithelial hepatocytes. These primary cell types have potential commercial value in a wide range of emerging technologies and therapeutic applications. Among these are: tissue engineering applications, such as would healing and burn treatment; high throughput screening for drug toxicology and efficacy studies; gene therapy; pseudo-organs (e.g.. artificial liver) and as model cell systems for developing new therapeutic approaches to human diseases.