SRM UNIVERSITY DEPARTMENT OF BIOTECHNOLOGY SCHOOL OF BIOENGINEERING Subject:BioprocessEngineering Semester: VI Subjectcode:BT0306 Year:2011 LECTURE SCHEDULE

S.No Hours to be taken Topic / Content Books Learning outcomes

UNIT I DESIGN AND ANALYSIS OF BIOREACTORS 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 1 1 1 1 1 1 1 1 1 1 Introduction to ideal reactors Batch reactor - performance equation CSTR performance equations Plug flow reactor- performance equation Modeling of Non-ideal Behavior in Bioreactors Problem & solution Tanks-in-series model Dispersion models with chemical reaction Applications to design of continuous sterilizers Problem & solution Air-lift loop reactors Fluidized bed bioreactors Stability analysis of bioreactorsProblem & solution UNIT II BIOREACTOR SCALE-UP 11. 12. 13. 14. 15. 16. 17. 1 1 1 1 1 1 1 Transport phenomena in Bioprocess systems Introduction to Heat & Mass transfer Oxygen transfer in bioreactor KLa Determination methods Static method - Problem & solution KLa Determination methods Dynamic method - Problem & solution Factors affecting KLa KLa Correlations for oxygen transfer Scale-up criteria Aeration and Agitation 3&6 3&5 3&7 3&7 3 3&7 3,4&7 To learn about the mass and heat transfer in bioreactor system, oxygen transfer from gas bubble to cells, determination of kLa in bioreactor. To study about the scale up of bioreactors on the basis of aeration and agitation. 1&2 1&2 1&2 1&2 1&2 1&2 6&8 3&8 1&2 5&6 To understand the rate, order of the reaction, Conversion of the reactant in batch, CSTR and PFR, determination of RTD in reactors. To learn about the tank in series, dispersion model and its application for sterilizer design. To study about the design of ALR, FBR and stability of bioreactors

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1 1 1

Regime analysis in bioreactor Power requirement for bioreactor Scale up- Problems & solutions UNIT III MONITORING OF BIOPROCESSES Introduction to methods of analysis in bioprocess Online , Offline methods On-line data analysis for measurement of important physical parameters On-line data analysis for measurement of important chemical & biochemical parameters Methods of on-line and off-line biomass estimation Microbial calorimetry Flow injection analysis State and parameter estimation techniques Computers and interfaces Computer-based data acquisition, monitoring and control LABVIEW Software

3,4&7 3,4&7 3,4&7

21. 22. 23. 24. 25. 26. 27. 28. 29. 30.

1 1 1 1 1 1 1 1 1 1

4&5 4,5&8 To study about the control 4,5&8 4&8 8 8 3&5 5 3 8 and monitoring of various parameters in bioreactor. To learn about the application of computers in controlling and monitoring of bioprocess.

UNIT IV MODERN BIOTECHNOLOGICAL PROCESSES 31. 32. 33. 34. 35. 36. 37. 38. 39. 1 1 1 1 1 1 1 1 1 Introduction to Recombinant cell culture processes Guidelines for choosing host-vector systems Plasmid stability in recombinant cell culture Problem & solution Modelling of recombinant bacterial cultures Bioreactor formation Bioprocess design considerations for animal cell cultures Bioprocess design considerations for animal cell cultures Bioprocess design considerations for plant cell cultures Bioprocess design considerations for plant cell cultures strategies for maximizing product 4 4 3 5 4 4 4 4 4 To understand about the recombinant cell cultures and its stability in bioreactor. To study about the bioreactor design consideration for animal and plant cell cultures.

UNIT V MODELLING AND SIMULATION OF BIOPROCESSES 40. 1 Introduction to Structured & Unstructured model for various bioprocesses 5&6 To understand about the structured model for

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1 1 1 1 1 1 1 1

Structured compartment model Williams model Structured compartment model Ramakrishna model Single cell model Metabolic model Genetically structured model Model simulation using MATLAB SIMULINK ISIM software packages

5&6 5&6 5&6 5&6 5&6 8 8 8

metabolism, growth of the microorganism using mathematical expressions. To learn about the simulation of bioprocess by using software like MATLAB, SIMULINK and ISIM

Text Books
Chemical Reaction Engineering Elements of Chemical Reaction Engineering Bioprocess Engineering Principles Bioprocess Engineering Basic Concepts

Octave Levenspiel

H. Scott Fogler

Pauline M.Doran

Michael L. Shuler Fikret Kargi

,

Biochemical Engineering Fundamentals

James E. Bailey David F. Ollis

,

Biochemical engineering Fundamentals of Biochemical Engineering Course Materials

Harvey W. Blanch, Douglas S. Clark

7 8

Rajiv Dutta

Name of the Staff Mr.C.Muthukumaran (Subject Coordinator) Mrs.M.Pandimadevi Mrs.P.Radha Mrs.G.Sharmila

Department Biotechnology Biotechnology Biotechnology Biotechnology

Email ID muthukumaranc@ktr.srmuniv.ac.in pandimadevim@ktr.srmuniv.ac.in radhap@ktr.srmuniv.ac.in sharmilag@ktr.srmuniv.ac.in

Contact Number 9894595995 9444145987 9443599966 9940596128

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