AULA

Bioquimica II

1. Converso de piruvato a ace7l-CoA: o complexo piruvato desidrogenase 2. Metabolismo do ace7l-CoA 3. Ciclo de Krebs : um ciclo anblico 4. As diferentes etapas de regulao 5. Produo de ATP e de NADH

Complexo piruvato desidrogenase piruvato CoA NAD+ NADH

ace7l-CoA

O complexo piruvato desidrogenase

Regulao da ac;vidade do complexo PDH

1. 2. 3. Produto = NADH e ace7l-CoA Por fosforilao = cinases / fosfatases Inibidores: compostos com arsnio

HS R' As O

H2O R' As

+
HS R

S R

Organic arsenicals are potent inhibitors of lipoamidecontaining enzymes such as Pyruvate Dehydrogenase. These highly toxic compounds react with vicinal dithiols such as the functional group of lipoamide.

ace7l-CoA C2 + Oxaloacetato C4

Citrato C6

CICLO DE KREBS

SINTESE DO CITRATO

citrato sintetase citrato sintetase

C2

C4

C6

CO2 C5

C4 CO2

C2

C4
NADH

C6
NADH

CO2 C5
FADH2 GTP NADH

C4 CO2

The Nobel Prize in Physiology or Medicine 1953

for his discovery of the citric acid cycle

Hans KREBS

for his discovery of co-enzyme A and its importance for intermediary metabolism
"

Fritz Lipmann

In 1937 Krebs was able to demonstrate the existence of a cycle of chemical reac7ons that combines the end-product of sugar breakdown, later shown to be an ac7vated form of the two-carbon ace;c acid, with the four-carbon oxaloace;c acid to form citric acid. The cycle regenerates oxaloace;c acid while libera7ng carbon dioxide and electrons The electrons are immediately u7lized to form adenosine triphosphate (ATP).
Extracto de uma biograa de H.Krebs

1 modelo do ciclo dos cidos tricarboxlicos

piruvato citrato oxaloacetato