Biotechnology Frontier

September 2013, Volume 2, Issue 3, PP.30-36

Calcium Phosphate and Danshen Compound

Coating on True Bone Ceramic
Hongjiang Jiang 1*, Xunxiang Tan 1, Haiyang Ju 1, XiuGang Song 1, Zhiye Qiu 2
1. Wendeng Hospital of Traditional Chinese Orthopedics and Traumatology, Wendeng Shandong 264400, China
2. Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, China

Abstract
The present work is to investigate the physicochemical and biological properties of true bone ceramic coated with calcium
phosphate/Danshen compound, and offer an ideal scaffold material for bone tissue engineering. In the study, surface coated true
bone ceramic were obtained by fully mixed with Calcium phosphate/Danshen compound and sucked under negative pressure.
Scanning electron microscopy (SEM), X-ray diffraction (XRD) and energy dispersive spectroscopy (EDS) were employed to
study the coated composition. The scaffold was co-cultured with osteoblast derived from bone marrow stromal cells, alkaline
phosphatase (ALP) activity at 2, 4, 6 and 8 days of cultivation were measured. The results indicated that there was no obvious
change in ingredient and Ca/P ratio of surface coated true bone ceramic, and the natural three-dimensional porous structure was
remained. The ALP activity was higher in the surface coated group than in the control group, indicated that the surface coated true
bone ceramic significantly promoted cells adhesion, proliferation and secretion. Therefore, bioactivity of true bone ceramic was
increased by the surface coating of calcium phosphate/Danshen compound, such coating composition with seed cells is an ideal
material for bone repair.
Key Words: True Bone Ceramic; Calcium Phosphate; Danshen; Surface Coating; Osteoblast

1* 1 1 1 2
1. 264400
2. 100084

X XRD X EDS
SEM ALP
Ca/P
ALP

True bone ceramicTBC(HA)

2005-2006-83
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1.1
NaOH30 % H2O2CaCl2LiCl 2 ml 2 g
calcium phosphate cementCPC
SXL-1208 X Rigaku-D/Max-2500 XL30E

1.2

(1) 0.5 mo1/L NaOH12 h(2) 1:1 1 h(3) 30% H2O224 h(4) 2 mol/L CaCl2
1 h(5) 8 mol/L LiCl1 h40 C 3
800 C 3 h

1.3
2 g CPC 1000 mg/mlpH 7.35
X
XRD X EDSSEM

1.4
1.4.1
16 1 ~ 2 ml 1640
2106/ml 2 50 ml 37 C
5% CO2
4 0.25% 2 15%
50 mg/L C10-8 mol/L 1640
1.4.2
5mm10mm10mm 24
2108/ml 24
2 36 1 D-Hanks 2%
1 CO2
246 8 6
ADZIA-2400 SIEMENS ALP

2.1

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 1 196 MPa

2.2 TBC
XHA
HACaCO32XTBC1.67
CaP3

(a)

(b)

2 X ab

(a)

(b)

3 ab
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2.3 TBC
SEMTBC
50260 m300500 m
4

(a)

(b)

(c)

(d)

4 SEM a50b100c
2000d2000

2.4
2.4.1

48

5 100
2.4.2
3

6
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(a)

(b)

(c)

(d)

6 a TBC 3db TBC

3dc TBC 6dd TBC 6d
2.4.3ALP
ALP t 246 8 ALP
P <0.01
1 ALP U/ml, n = 6, x s

2d

4d

6d

8d

1.2 0.21

1.6 0.31

2.3 0.15

3.8 0.35

1.8 0.17*

2.4 0.12*

3.2 0.32*

5.3 0. 19*

*P < 0.01

HA

HA
[1-5]
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[6-8]

CPC
CPC[9-14]CPC

[15-18]CPC

ECMextracelluar matrix

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