Fig 28-1:
• Similar to FIA, GC
• Introduce small sample (0.1-100 µL) without depressurization
• Microsyringe/septum system (only <1500 psi)
Analyte Polarity:
hydrocarbons<ethers<esters<ketones<aldehydes<amines<alcohols
= 10 ( 2 1 )
k' 2 P' −P' /2
k' 1
{t = V + V
{i +
V V
{g {o
total gel or inside outside
solid pores pores/
free−space
Fig 28-27
• Separation of proteins/peptides, sugars, determination of polymer
molecular weight distribution