Potentiometric Measurements
Objectives
1. 2. 3. To have an understanding of potentiometric measurements using various kind of electrode To determine and compare the pH of different samples using standard pH meter To determine and compare the amount of fluoride ion in different samples of toothpaste and mouth wash using Fluoride-ion selective electrode.
Reagents
Buffer solutions (pH = 4, 7 and 9) shampoo samples TISAB (total ionic strength adjustment buffer) solution 0.1 M F stock standard solution
Apparatus
beakers Pipets Iron stand Iron clamp Fluoride-ion selective electrode burette pH meter volumetric flask
Procedure
I.
Potentiometric Measurements
B. Determination of fluoride from sample using Calibration Curve Method 1. Prepare 5 ppm fluoride solution by diluting 25.0 mL of the 100 ppm standard to 500 mL in a volumetric flask. 2. Transfer 5.00, 10.0, 25.0 and 50.0 mL aliquots of the 5 ppm solution to 100 mL volumetric flask, add 50 mL of TISAB solution and dilute to the mark. These solutions correspond to 0.5, 1.0, 2.5, and 5.00 ppm fluoride ion in the sample). 3. Determine the potential of each solution and prepare the calibration curve (E vs. log C) 4. Determine the potential of the samples 5. From the graph determine the concentration of fluoride in the sample.
C. Determination of fluoride from tap water sample using Standard Addition Method 1. Prepare the following solutions in a 100 mL volumetric flask by pipetting known volume of previously prepared standard solution (5 ppm): Solution Composition 1 50 mL TISAB + 5 mL tap water + 0 mL standard 2 50 mL TISAB + 5 mL tap water + 10 mL standard 3 50 mL TISAB + 5 mL tap water + 20 mL standard 4 50 mL TISAB + 5 mL tap water + 30 mL standard 2. 3. 4. Measure the potential of each solution. Plot the E vs (CV)std Determine the concentration of fluoride in the sample.
Experiment No. 1
Potentiometric Measurements
Group No.
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Date:
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Name:
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D A T A
I. pH Determination of various shampoos
S H E E T
Experiment No. 1
Potentiometric Measurements
Solution Number
Solution Number
slope
Linearity (R)
E (mV)
[F-]
Sample
E (mV)
[F-]
Mouthwash
Tap water
Experiment No. 1
Potentiometric Measurements
Solution Number
Vo
Vstd
CstdVstd
E, mV
Professors Signature