Exercise 1: Cell Transport Mechanisms and Permeability: Activity 2: Simulated Facilitated Diffusion Lab Report Pre-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly. 1. Molecules need a carrier protein to help them move across a membrane because You correctly answered: d. they are lipid insoluble or they are too large. 2. Which of the following is true of facilitated diffusion? You correctly answered: c. Movement is passive and down a concentration gradient. 3. Examples of solutes that might require facilitated diffusion include You correctly answered: d. all of the above. 4. Which of the following would not affect the rate of facilitated diffusion? You correctly answered: a. the amount of intracellular ATP

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Experiment Results Predict Question: Predict Question 1: What effect do you think increasing the number of protein carriers will have on the glucose transport rate? Your answer : b. The glucose transport rate will increase. Predict Question 2: What effect do you think adding Na+ Cl- will have on the glucose transport rate? Your answer : c. There will be no change in the glucose transport rate. Stop & Think Questions: What happened to the glucose transport rate when the glucose concentration was increased from 2 mM to 8 mM? You correctly answered: a. The glucose transport rate increased. Describe the conditions that resulted in the fastest rate of glucose transport. You correctly answered: d. 700 glucose carrier proteins and 8 mM glucose Why do you think equilibrium wasn't reached? You correctly answered: b. The membrane proteins were saturated. Experiment Data: Run Number 1 2 3 4 5 6 6 Solute Glucose Glucose Glucose Glucose Glucose Na+ ClGlucose Start Conc. L 2.00 8.00 2.00 8.00 10.00 0.00 2.00 Start Conc. R 0.00 0.00 0.00 0.00 0.00 2.00 0.00 Carriers 500 500 700 700 100 700 700 Rate 0.0008 0.0023 0.0010 0.0031 0.0017# 0.0042 0.0010

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Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. What variable(s) increased the rate of facilitated diffusion of glucose? You correctly answered: d. both a and b 2. For facilitated diffusion, increasing the concentration of glucose on one side of the membrane is the same as You correctly answered: b. making the concentration gradient steeper 3. When all of the membrane carriers are engaged, or busy, we say they are You correctly answered: a. saturated. 4. Na+ Cl- had no effect on glucose transport because You correctly answered: b. sodium is not required for glucose transport in the simulation.

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Review Sheet Results 1. Explain one way in which facilitated diffusion is the same as simple diffusion and one way in which it is different from simple diffusion. Your answer: 1.) Same as simple diffusion - They are both going from a high concentration to a low concentration gradient. (Downward movement) 2.) Different from simple diffusion - Facilitated diffusion is helped by an integral protein that form a molecule-specific channel. Simple diffusion does not need a protein. 2. The larger value obtained when more glucose carriers were present corresponds to an increase in the rate of glucose transport. Explain why the rate increased. How well did the results compare with your prediction? Your answer: The number of carrier proteins increased the rate of glucose transport because more proteins help get the diffusion process speed up. My prediction was correct. 3. Explain your prediction for the effect Na+ Cl- might have on glucose transport. In other words, explain why you picked the choice that you did. How well did the results compare with your prediction? Your answer: NaCl had no effect on the rate of glucose transport because it has no effect or has no need of the carrier protein. My results reflect my prediction.

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