For life science research only. Not for use in diagnostic procedures.

FOR IN VITRO USE ONLY.

DNA Molecular Weight Marker XIV (1001500 bp)
100 base pair ladder
Cat. No. 11 721 933 001 Version Sept. 2005
50 g c 1 A
260
unit
Store at 15 to 25C
50 g (200 l) for 50 gel lanes
Product overview
Formulation Solution in 10 mM Tris-HCl, 1 mM EDTA, pH 8
Concentration 250 g/ml
Size distribution Fragment mixture prepared by cleavage of a specially
constructed plasmid with restriction endonucleases.
The mixture contains 15 double stranded DNA frag-
ments with the following base pair lengths:
100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100,
1200, 1300, 1400, 1500 and an additional band of 2642
bp. The 250 bp and 500 bp banding pattern is two to
three times brighter. The 500 bp and 1000 bp banding
pattern is two to three times brighter
Application The DNA Molecular Weight Marker XIV allows accurate
sizing of DNA fragments generated by PCR or restric-
tion digest separated on agarose gels. The DNA molec-
ular weight marker can be used in conjunction with
DNA Molecular Weight Marker XIII (50 bp ladder)* for
precise size determination.
Electrophoretic separation of this molecular weight
marker results in a regular pattern. The fragments have
5'-protruding ends which can be labeled by filling in
reactions using e.g. [
32
P]-dTTP and [
32
P]-dGTP, or using
DIG-11-ddUTP* and Terminal Transferase*.
Typical analysis The DNA fragment mixture shows the ladder with 15
bands and an additional band of 2642 bp in agarose gel
electrophoresis as shown in the figure.
Separation
conditions
Apply 1 g DNA per lane on a 2% Agarose MP* gel.
Storage/stability The unopened vial is stable at 15 to 25C until the
expiration date printed on the label
Note: This product is shipped in dry ice and should be
stored at 15 to 25C until use.
Once thawed we recommend further storage at +2 to
+ 8C. Repeated freezing and thawing should be
avoided.

* available from Roche Applied Science
Fig.: Separation of 1 g DNA molecular weight marker XIV on a 2%
agarose gel. Ethidiumbromide stain..
Printed Materials
Contact and Support
To ask questions, solve problems, suggest enhancements or report new
applications, please visit our Online Technical Support Site at:
www.roche-applied-science.com/support
To call, write, fax, or email us, visit the Roche Applied Science home page,
www.roche-applied-science.com, and select your home country. Country-
specific contact information will be displayed. Use the Product Search func-
tion to find Pack Inserts and Material Safety Data Sheets.
LabFAQs "Find a Quick Solution"
Molecular Weight Markers for Nucleic Acids
or available at
http://www.roche-applied-science.com/
prod_inf/applic/mwm.pdf
Restriction Enzyme Ordering Guide
Restriction Enzyme Poster
0905.11727290
Roche Diagnostics GmbH
Roche Applied Science
68298 Mannheim
Germany