MACROMOLECULAR STRUCTURE AND ANALYSIS ( SEMESTER - 1 )

CS/B.Sc(H) (Genetics/BT/Mol.bio,Micro.bio)/SEM-1/MSA-101 /08/(09)

1.
Signature of Invigilator

2.
Signature of the Officer-in-Charge

Reg. No.

Roll No. of the Candidate

CS/B.Sc(H) (Genetics/BT/Mol.bio,Micro.bio)/SEM-1/MSA-101/08/(09) ENGINEERING & MANAGEMENT EXAMINATIONS, DECEMBER 2008

MACROMOLECULAR STRUCTURE AND ANALYSIS ( SEMESTER - 1 )

Time : 3 Hours ] [ Full Marks : 70

INSTRUCTIONS TO THE CANDIDATES :

1. 2. This Booklet is a Question-cum-Answer Booklet. The Booklet consists of 32 pages . The questions of this concerned subject commence from Page No. 3. a) In Group A, Questions are of Multiple Choice type. You have to write the correct choice in the box provided against each question. b) For Groups B & C you have to answer the questions in the space provided marked Answer Sheet. Questions of Group B are Short answer type. Questions of Group C are Long answer type. Write on both sides of the paper. Fill in your Roll No. in the box provided as in your Admit Card before answering the questions. Read the instructions given inside carefully before answering. You should not forget to write the corresponding question numbers while answering. Do not write your name or put any special mark in the booklet that may disclose your identity, which will render you liable to disqualification. Any candidate found copying will be subject to Disciplinary Action under the relevant rules. Use of Mobile Phone and Programmable Calculator is totally prohibited in the examination hall. You should return the booklet to the invigilator at the end of the examination and should not take any page of this booklet with you outside the examination hall, which will lead to disqualification. Rough work, if necessary is to be done in this booklet only and cross it through.

3. 4. 5. 6.

7. 8. 9.

No additional sheets are to be used and no loose paper will be provided

FOR OFFICE USE / EVALUATION ONLY

Marks Obtained
Group A Question Number Marks Obtained Group B Group C Total Marks Examiner's Signature

Head-Examiner/Co-Ordinator/Scrutineer 1101 ( 4/12 )

CS/B.Sc(H) (Geneti cs/BT/Mol.bio,Micro.bio)/SEM-1/MSA-101/08/(09)

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CS/B.Sc(H) (Geneti cs/BT/Mol.bio,Micro.bio)/SEM-1/MSA-101/08/(09)

ENGINEERING & MANAGEMENT EXAMINATIONS, DECEMBER 2008

MACROMOLECULAR STRUCTURE AND ANALYSIS

SEMESTER - 1
Time : 3 Hours ] GROUP A ( Multiple Choice Type Questions ) 1. Choose the correct alternatives for any ten of the following : i) Co-enzyme A contains the vitamin a) c) ii) Riboflavin Pyridoxal b) d) Pantothenic acid Thiamine. 10 ! 1 = 10 [ Full Marks : 70

Amino acids capable of being utilized by human body belogns to a) c) D-Series Both (a) and (b) b) d) L-Series None of these.

iii)

Polymers of more than 100 amino acids are termed as a) c) Protein only Both proteins & polypeptides b) d) Polypeptides only None of these.

iv)

Shine-Dalgarno sequence are responsible for a) c) transcription transformation b) d) translation replication.

v)

Which of the following amino acid is specified by only a simple codon ? a) c) Glutamine Aspargine b) d) Tryptophan None of these.

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vi)

4 An antib iotic that resembles the 3' end of a charged t-RNA molecule is a) c) Streptomycin Sparsomycin b) d) Penicillin Puromycin.

vii)

DNA that is located within genes and is not used to specify a product but is discarded in RNA processing is known as a) c) Intron DNA Spacer DNA b) d) Exon DNA Regulator DNA.

viii)

Which of the cheimcal mutagens are likely to cause frameshift mutations ? a) c) 5-Bromo uracil Acrydine organge b) d) 2-amino purine HNO 2 .

ix)

Anomeric C atom in glucose is a) c) C-1 C-6 b) d) C-2 C-5.

x)

The kind of nucleic acid that is represented by cloverleaf model is a) c) Mitochondrial DNA t-RNAd) b) Chromosomal DNA

m-RNA.

xi)

The macro molecule whose monomer are bound by no covalent forces a) c) Carbohydrates Both (a) & (b) b) d) Lipids None of these.

xii)

Guanosine is a) c) Nitrogenous base Ribonucleotide b) d) Ribonucleoside None of these.

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5 GROUP B ( Short Answer Type Questions ) Answer any three of the following : 2. 3 ! 5 = 15

Write the chemical names of guanine and thymine and write their structures. How do nucleosides differ from nucleotides ? What kind of bond connects adjacent nucleotides on the same polynucleotide strand ? 3 +1+ 1

3.

a) b)

Write down the structure of Glu and His at pH 80. What happens when protein solution is heated ? [(2 +2)+1 ] 2!2 1 2

4.

Write short notes on the following : a) b) Allosteric enzymes. Isozymes.

5. 6. 7.

Design an experiment to prove that the genetic material is DNA, not protein. Describe Watson - Crick Model of DNA. Explain the following mutations : a) b) missense, silent. What is mutagen ? Name two mutagens. GROUP C ( Long Answer Type Questions ) Answer any three of the following.

5 5

3+2

3 ! 15 = 45

8.

What are lipids ? How are they classified ? Name two important phospholipids. What are liposomes ? Write down the b asic structures of biological membrane. What is lipoprotein & its application ? 1 +2+ 2+2+ 3+5

9.

Many proteins absorb UV light at 280 nm yet gelatin not. Why ? Why is phenylalanine poorly soluble in water while serine is highly water solubtle ? Which amino acid can be converted into different amino acids by mild hydrolysis with the liberation of ammonia ? Write down ninhydrin test. What do you mean by motif and domain ? 3 +2+ 1+4+ 5

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10.

6 Why is " factor important in tr anscription in prokaryotes ? Why are transcription and translation coupled in prokaryotic cell ? How does initiator met t-RNA met differ from the same present in between a synthesizing protein sequence ? Write short note on Shine-Dalgarno sequence. 3 +5+ 3+4

11.

a)

Write short notes on any two of the following : i) ii) iii) Lethal mutation Induced mutation Non-sense mutation. ( 2 !5 )+ 5

b) 12.

What are the phenotypic effects of mutation ?

What are the three principal elements in m-RNA sequence in bacterial cells ? What is 5l cap ? How is 5l cap added to eukaryotic pre m-RNA ? How introns are spliced ? 5 +2+ 4+4

13.

a) b) c)

Why is m-RNA polyadenylated ? What is meant by hyperchromic shift of DNA ? Discuss the structure and function of sphingolipid and phospholipid. 3 +4+ (4+4 )

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