Code No: RR312302 Set No.

1
III B.Tech I Semester Supplementary Examinations, February 2007
GENETIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. What is attenuation? How does it help in controlling gene expression? [4+12]

2. Explain the expression of hormone regulated genes in eukaryotes [6+10]

3. Explain the structure of PBR322. [4+12]

4. Write notes on any two:

(a) Ploylinker DNA

(b) Staggered cuts
(c) λ - Phage as vector [8+8]

5. What are probes? Discuss their importance in cloned gene detection. [4+12]

6. What is the basic principle of PCR? Write about the different types of PCR. [6+10]

7. Comment on the applications of molecular markers in various fields of biotechnol-

ogy. [4+12]

8. Explain the detailed structure of a Ti plasmid in Agrobacterium. [6+10]

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Code No: RR312302 Set No. 2
III B.Tech I Semester Supplementary Examinations, February 2007
GENETIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Write notes on any two

(a) CAP- binding site

(b) Translation regulation
(c) Regulator [8+8]

2. Write notes on any two:

(a) Steriod hormonal regulation

(b) Short repeats in DNA
(c) DNA binding motifs. [8+8]

3. What are the characters of ideal plasmid? Explain with suitable example. [8+8]

4. Write notes on any two:

(a) Restriction digestion

(b) Homopolymer tailing
(c) Ligases [8+8]

5. Write the steps involved in construction of a genomic library. [4+12]

6. Explain the role of PCR in Forensic Science. [4+12]

7. Comment on the applications of molecular markers in various fields of biotechnol-

ogy. [4+12]

8. Describe the construction of plant vectors based on Agrobacterium Ti plasmid.

[6+10]

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Code No: RR312302 Set No. 3
III B.Tech I Semester Supplementary Examinations, February 2007
GENETIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Write about Arabinose operon regulation in Prokaryotes. [4+12]

2. Write notes on any two:

(a) Tissue specific enhancers

(b) Phosphorylated proteins
(c) TATA box [8+8]

3. Write notes on any two:

(a) Relaxed plasmid

(b) PUC8
(c) α - Complementation. [8+8]

4. Write detailed account on restriction mapping and its importance. [4+12]

5. Explain nucleic acid hybridization and its uses as a screening procedure. [6+10]

6. What is the basic principle of PCR? Write about the different types of PCR. [6+10]

7. What is gene mapping? Explain the role of restriction enzymes in it. [6+10]

8. Elaborate on the importance of transgenic animals citing suitable examples. [8+8]

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Code No: RR312302 Set No. 4
III B.Tech I Semester Supplementary Examinations, February 2007
GENETIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Differentiate between positive and negative control in lac operon. [8+8]

2. Write notes on any two:

(a) Silencers
(b) Transacting regulatory proteins
(c) Britton and Davidson model [8+8]

3. Write notes on any two:

(a) Mechanism of transpositions

(b) Isolation of plasmids
(c) Ty elements in yeast. [8+8]

4. Give the methods of isolation and purification of DNA from plant systems. [6+10]

5. Give a brief definition of a gene library. What is the essential difference between
a genomic library and a cDNA library? List the major advantages/limitations on
the use of each. [8+8]

6. Discuss about Multiplex PCR. [4+12]

7. Write short notes on any two:

(a) Satellite markers

(b) Restriction enzymes
(c) Gel electrophoresis. [8+8]

8. Discuss about the viral methods used in doing In vivo gene therapy. [4+12]

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