Aptio Automation Practical Exercise

Aptio Automation
Sample Processing
Approximate Time Required: 30 minutes Technical References: Aptio Automation Operators Guide Educational References: Aptio Automation Sample Processing Online Training Objectives: Upon completion of this exercise you will be able to perform the following tasks: Identify sample requirements Load and unload samples from the Input/Output Module Describe the sample processing sequence Search for samples in the database Deliver a sample to the Input/Output Module

Supplies Needed: Personal Protective Equipment as determined by your local regulations 15- 20 sample tubes with barcodes and work orders downloaded to Aptio Automation. Use both pre-spun and unspun sample tubes to complete this exercise. If your system is configured to load uncapped samples, use some of these as well. Helpful Hints: To avoid the disruption of laboratory workflow, consult with the Aptio Automation primary operator before starting this practical exercise. Complete the Aptio Automation Sample Processing Online Training before starting this Practical Exercise. If your Aptio Automation does not have a Centrifuge or Decapper module, use the appropriate sample tube type (Ex. spun, uncapped) for your system. Ensure that output racks of the appropriate type are available in the Input/Output Module before beginning the exercise. An answer key is provided at the end of this exercise. Please give these pages to your mentor prior to starting the practical exercise.

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Exercise Activity and Questions Identify sample requirements Aptio Automation is configured based on the laboratorys needs. For a list of approved sample tube types, refer to the Aptio Automation Operators Guide. 1. Below are examples of sample containers found in the laboratory. For each one, state if they are acceptable for processing on your system and if not, the reason why. a. Glass tubes

b. 13X100 round bottom plastic tubes

c. Flat bottom plastic tubes

d. Sample cup nested inside of a 13X100 plastic tube

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Load and unload samples from the Input/Output Module Note: Consult with the primary operator before performing these exercises so as not to disrupt laboratory operations. Input/Output Module lane configurations vary based on the laboratorys needs. Not all of the LED icons described below may be configured on your system. These exercises are designed for a system with both a Centrifuge and Decapper Module. If your system does not have these modules, load the appropriate sample tube types for your system. 2. When loading samples, it is important that the samples introduced into an input lane have the appropriate centrifugation and cap status designated for that lane. Next to each icon below, write the workorder status, centrifugation, and cap status of samples that are loaded into that lane.

LED Icon

Priority or Routine

Spun or Unspun

Capped or Uncapped

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3. When operating Aptio Automation it is important to have output racks in the Input/Output Module to allow the Input/Output Robot to unload samples. In the table below, enter the letter of the description for the icon shown. Answer LED Icon Description A. All sample tube routing is complete.

B. Parked temporarily: sample tube routing is not complete

C. Samples with errors requiring operator intervention

D. Sorted according to rules

4. Determine which lanes on your Input/Output Module are designated for output racks and load empty racks into them. a. What action does the robot take after the rack has been inserted?

b. What color is the LED button above the output lane when an output rack is present?

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5. Ensure that all modules and instruments required to process your samples are online. a. Which screen in the User Interface displays all the module and instrument statuses?

b. In this screen, what color indicates that a module or instrument is available to process samples?

6. Select 5 Routine (capped, unspun) samples and record the sample IDs. Load them into a 48 tube sample rack. Note: If your system does not have a Centrifuge or Decapper Module, load the appropriate sample tube type for your system. a. Into which column and row do you place the first sample?

b. What pattern should you follow to load the rest of the samples? (columns and rows)

c. Why is it important to avoid empty spaces between samples in each column of the rack?

d. Should spun and unspun sample tubes be loaded into the same rack? If not, why?

7. Identify the appropriate Input/Output Module lane for loading these samples. a. What is the lane type into which this rack should be loaded?

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8. Load the sample rack into a lane and observe the IOM Robot loading the samples. a. From which column and row does the robot start loading samples?

b. Which additional sample rack positions are checked after all sample tubes have been loaded onto the track?

9. Select at least 4 Routine and 2 Priority samples and record the sample IDs. Load the Routine samples into one rack and the Priority samples into another rack. Position both racks in front of the appropriate lanes. DO NOT INSERT. When ready, insert the Routine rack first. Once the robot has started loading the samples, insert the Priority samples into the Priority Input Lane.

a. What action does the IOM Robot take once the rack is inserted into the Priority Input lane?

b. What action does the IOM Robot take once all the samples are loaded from the Priority Input lane?

10. If available, load pre-spun samples onto your system. a. Which lane statuses are appropriate for samples that are spun and capped?

b. Which lane statuses are appropriate for samples that are spun and uncapped?

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11. When the samples have completed processing, determine which output racks are available for removal. Check for sample errors before removing the rack. Note: Systems with a Refrigerated Storage Module may not send completed samples to the Input/Output Module. a. List the steps to check for sample errors in the User Interface.

b. What action should you take before removing an unload rack?

c. Perform the action from the last step. List the steps below.

d. Unlock and remove the rack. List the steps below.

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Describe the sample processing sequence 12. Load a few Routine capped, unspun sample tubes into a rack. Insert the rack into Input/Output Module and observe the samples as they travel around the track. a. List the sequence of the components encountered in order. (For example: Centrifuge Module, Decapper Module, analyzer, Sealer Module and Input/Output Module)

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Search for samples in the database 13. Select a sample ID recorded earlier and search for that sample in the database. a. List the steps to locate sample information.

b. What is the current location of the sample?

c. What information is displayed when you select the checkmark at the bottom?

Deliver a sample to the Input/Output Module 14. Obtain the sample ID for a sample on the system. (Note: you may need to load an additional sample) Request the sample to be delivered. a. Which button do you select from the Overview screen in order to initiate delivery of a sample?

b. List the steps you took in the software to perform the process.

c. Into which type of lane was the sample delivered?

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Exercise Checklist Completed Task Identify sample requirements Load and unload samples from the Input/Output Module Describe the sample processing sequence Search for samples in the database Deliver a sample to the Input/Output Module

Exercise Completed: Name: ____________________________ Signature: _________________________ Date: _____________________________ Mentor: ______________________________ Title: ________________________________ Signature: ____________________________

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Exercise Activity Answers NOTE: The following pages are the answers to the Practical Exercise questions and activities Please give these pages to your mentor prior to starting the practical exercise

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Exercise Activity Answers Identify sample requirements 1.a. Not acceptable if a Sealer or Bulk Input Module is present on the system. b. Acceptable c. Not acceptable, sample carrier is not designed for flat bottomed tubes d. Not acceptable, the IOM Robot is not designed to pick up nested sample cups

Load and unload samples from the Input/Output Module 2. LED Icon Priority or Routine Priority Spun or Unspun Unspun Capped or Uncapped Capped

Priority

Spun

Uncapped

Priority

Spun

Capped

Routine

Unspun

Capped

Routine

Spun

Uncapped

Routine

Spun

Capped

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3. Answer D B. Parked temporarily: sample tube routing is not complete C C. Samples with errors requiring operator intervention A D. Sorted according to rules B LED Icon Description A. All sample tube routing is complete.

4.a. Checks for sample tubes in positions A1, B1, C1, D1 b. Green or dark green 5.a. Overview screen b. Green 6. a. A1 b. A2 - A12, B1-B12, C1-C12, D1-D12 c. IOM Robot will stop searching for samples in the column once an empty space is detected. d. No, each lane is designated for either spun or unspun sample tubes so the system can determine which samples require centrifugation. 7.a. Routine Input 8.a. A1 b. B1,C1.D1

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9. a. IOM Robot stops loading samples from the Routine Input lane and loads samples from the Priority Input lane. b. The IOM Robot resumes loading samples from the Routine Input lane. 10. a. Centrifuged Priority Input and Centrifuged Routine Input b. Uncapped Priority Input and Uncapped Routine Input 11. a. In the User Interface > Select Overview > Select the lane number > Review the samples for any errors b. Print a sample list for the rack c. In the User Interface > Select Overview > Select the lane number > Select Print d. Push the corresponding lane button on the LED bar. Using the handle, push the rack in gently to release the latch. Pull the rack out completely.

Describe the sample processing sequence 12. a. Varies by site and system configuration. Basic sample processing sequence follows: 1. IOM Robot picks up a sample from and Input rack and places it in a carrier on the track. Sample may also be loaded by the Rack Input or Bulk Input Modules. 2. Barcode is read and sample ID is paired with the carrier. 3. Sample arrives at Centrifuge Module where CM Robot picks up the sample and places it into a bucket insert. Sample is weighed, balanced and placed in the centrifuge. 4. After centrifugation is completed, CM Robot places sample into a carrier on the track. 5. Barcode is read and sample ID is paired with the carrier. 6. Sample arrives at the Decapper Module. Cap is removed. 7. Sample arrives at an instrument for analysis. The instrument receives the workorder from the middleware and processes the sample. 8. Sample travels to all instruments required to complete the workorder test requests. 9. Sample arrives at the Sealer Module and is sealed. 10.Sample arrives at the Input/Output, Rack Output or Refrigerated Storage module.

Search for samples in the database 13.a. Select Overview > Samples> Enter the Sample ID > Select Checkmark (confirm button) b. Site specific c. Test, analyzer, sender, status Deliver a sample to the Input/Output Module 14.a. Samples b. Select Samples> Enter the Sample ID > Select the Checkmark > Select Deliver > Select Selected Sample. c. Priority Output

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