Code: 9A23301

1
BIOCHEMISTRY
(Biotechnology)

B.Tech II Year I Semester (R09) Regular & Supplementary Examinations December/January 2013/14

Time: 3 hours Answer any FIVE questions All questions carry equal marks *****
1 (a) (b) (c) (d) (e) 2 3 4 5 6 7 8 (a) (b) Draw the Fischer projections of following sugars: D-fructose. D-ribose. D-mannose. D-glucose. D-galactose.

Max Marks: 70

Compare the induced fir model of enzyme activity with the lock and key model. Explain the various steps involved in glyconegenesis and glucognesis. Discuss about the electron carrier complexes in electron transport chain. What is reductive amination and transmination? Give two examples. How the aromatic amino acids synthesized? Write an account on structure and functions of fatty acids. What are the differences between DNA and RNA? Write an account on phospholipids and its functions. *****

Code: 9A23301

2
BIOCHEMISTRY
(Biotechnology)

B.Tech II Year I Semester (R09) Regular & Supplementary Examinations December/January 2013/14

Time: 3 hours Answer any FIVE questions All questions carry equal marks *****
1 2 3 4 5 6 Explain the effect of pH on enzyme activity. Describe the structure of triglycerides. Add a note on fates of pyruvate and give the energetic of glycolysis. Explain various reactions involved in anaerobic respiration. Classify amino acids and give their structures.

Max Marks: 70

Give a comprehensive account of the chemistry, biosynthesis and biochemical importance of cholesterol. What are: Purines. Pyrimidines. Nucleosides. Nucleotides. Detail the various physio- chemical properties of proteins.

7 (a) (b) (c) (d) 8

*****

Code: 9A23301

3
BIOCHEMISTRY
(Biotechnology)

B.Tech II Year I Semester (R09) Regular & Supplementary Examinations December/January 2013/14

Time: 3 hours Answer any FIVE questions All questions carry equal marks *****
1

Max Marks: 70

Give an account on structure and properties of polysaccharides and complex carbohydrates. Define the following terms: Glycolysis. Pentose phosphate pathway. Gluconeogenesis. Glycogenolysis. Derive the Michaelis-Menton equation and outline enzyme-substrate specificity. Explain various reactions involved in aerobic respiration. Describe urea cycle and give its metabolic significance. List out the reactions involved in glutamate pathway. What are essential fatty acids? Add a note on their significance. What are nucleic acids and how they are synthesized and degraded?

2 (a) (b) (c) (d) 3 4 5 6 7 8

*****

Code: 9A23301

4
BIOCHEMISTRY
(Biotechnology)

B.Tech II Year I Semester (R09) Regular & Supplementary Examinations December/January 2013/14

Time: 3 hours Answer any FIVE questions All questions carry equal marks *****
1 2 3 4 Describe the different functions of glycogen in liver and muscle. Define enzyme and outline how enzymes catalyse reactions. Explain the various steps involved in glycolytic pathway.

Max Marks: 70

State where and how the electron transport chain creates a proton gradient and explain how ATP is produced by chemiosmosis. Explain the structural organization of proteins and list four functions of proteins, giving an example of each. List out the reactions involved in serine pathway. Describe the pathway of synthesis of three ether linked lipids. (a) (b) Write down the differences between purines and pyrimidines. Draw and label a simple diagram to show how DNA is constructed from sugars, phosphates and bases. *****

6 7 8