Glucose GOD

Diagnostic reagent for quantitative in vitro determination of glucose in serum or plasma on photometric systems Order Information
Catalog No D1G25 125 D1G25 150 D1G25 300 D1G25 500 D1G25 600 D1G25 LT1 Kit size 5 x 25 ml 6 x 25 ml 12 x 25 ml 1 x 500 ml 6 x 100 ml 1000 ml [1,2] #aste Management $lease refer to local legal re)uirements. Reagent Preparation Beagent and standard are ready#to#use. Materials required !ut not provided Ca<l solution A g6l. !eneral laboratory e)uipment.

Summary

Measurement of glucose concentration in serum or plasma is mainly used in diagnosis and monitoring of treatment in diabetes mellitus. Other applications are the detection of neonatal hypoglycemia, the exclusion of pancreatic islet cell carcinoma as well as the evaluation of carbohydrate metabolism in various diseases.

Specimen
Derum, heparin plasma or E"(% plasma. Deparate at the latest 1h after blood collection from cellular contents. Dtability after addition of a glycolytic inhibitor +CaF, GF-& 1 day at 25924 ;< 3 days at *9: ;< "iscard contaminated specimens.

Method
!O"#$%$ & en'ymatic photometric test

Principle
"etermination of glucose after en'ymatic oxidation by glucose oxidase. (he colorimetric indicator is )uinoneimine, which is generated from *#aminoantipyrine and phenol by hydrogen peroxide under the catalytic action of peroxidase +(rinder,s reaction- [.].
!lucose / O2 !O" 0 !luconic acid / $henol / $O" 12O2 0 2uinoneimine / * 12O

$ssay Procedure
Application sheets for automated systems available on request. Havelength 455 nm, 1g 4*@ nm Optical path 1 cm (emperature 25924 ;< 6 .3 ;< Measurement %gainst reagent blan7 are

2 12O2 / *#%minoantipyrine

Reagents
Components and Concentrations in the Test Reagent $hosphate buffer p1 3.4 245 mmol6l $henol 4 mmol6l *#%minoantipyrine 5.4 mmol6l !lucose oxidase +!O" 15 786l $eroxidase +$O" 1 786l Standard 155 mg6dl +4.44 mmol6lStorage Instructions and Reagent Sta!ility (he reagent is stable up to the end of the indicated month of expiry, if stored at 29: ;<, protected from light and contamination is avoided. "o not free'e the reagents= "ote >t has to be mentioned, that the measurement is not influenced by occasionally occurring color changes, as long as the absorbance of the reagent is ? 5.. at 4*@ nm. (he standard is stable up to the end of the indicated month of expiry, if stored at 2924 ;<. #arnings and Precautions 1. (he reagent contains sodium a'ide +5.A4 g6l- as preservative. "o not swallow= %void contact with s7in and mucous membranes. 2. (a7e the necessary precautions for the use of laboratory reagents.

%lan& Sample or standard Sample or standard # 15 Il Dist' (ater 15 Il # Reagent 1555 Il 1555 Il Mix, incubate 25 min. at 25#24 ;< or 15 min. at .3 ;<. Bead absorbance against the blan7 within @5 min.

Calculation
Hith standard or calibrator.

!lucos e [mg 6 dl] =

% Dample <onc. Dtd 6 <al [m % Dtd 6 <al

Conversion factor !lucose [mg6dl] x 5.54441J !lucose [mmol6l]

Cali!rators and Controls

For the calibration of automated photometric systems the "iasis (ru<al 8 calibrator is recommended. For internal )uality control "iasis (ruKab C and $ controls should be assayed with each batch of samples.
""D <alibrator <ontrol C <ontrol % <at. Co. "1<14#5@5 "1<14#55A "1<35#155 "1<35#524 "1<34#155 "1<34#524 25 . 25 4 25 4 Git si'e x . x . x 4 x 4 x 4 x 4 ml ml ml ml ml ml

255*654 Bev & 55

!lucose !O" 9 $age 1

Performance Characteristics
Measuring range (he test has been developed to determine glucose concentrations within a measuring range from 1 9 *55 mg6dl. Hhen values exceed this range samples should be diluted 1 / * with Ca<l solution +A g6l- and the result multiplied by 4. Specificity ) Interferences %scorbic acid up to 14 mg6dl, bilirubin up to *5 mg6dl, hemoglobin up to 255 mg6dl and lipemia up to 2,555 mg6dl triglycerides observed no interference. Sensitivity ) *imit of Detection (he lower limit of detection is 1 mg6dl. Precision +at ,-.C/ >ntra#assay precision n J 25 Dample 1 Dample 2 Dample . >nter#assay precision n J 25 Dample 1 Dample 2 Dample . Mean [mg6dl] @*.2 122 2A@ Mean [mg6dl] A2.4 121 2A2 D" [mg6dl] 1.12 1.43 *.*1 D" [mg6dl] 1.15 1.52 2.51 <L [M] 1.3* 1.2: 1.*A <L [M] 1.1A 5.:* 5.@A

Reference Range
Cewborns& <ord blood 1h 2h 4 9 1* h 15 9 2: h ** 9 42 h <hildren +fasting-& 1 9 @ years 3 9 1A years %dults +fasting-& Lenous plasma

[1]

[mg6dl] @. # 14: .@ # AA .@ 9 :A .* 9 33 *@ 9 :1 *: 9 3A 3* 9 123 35 9 15@ 35 9 114

[mmol6l] ..4 # :.: 2.5 9 4.4 2.2 9 *.A 1.A 9 *.. 2.@ 9 *.4 2.3 9 *.* *.1 9 3.5 ..A 9 4.A ..A 9 @.*

*iterature
1. 2. (homas K. <linical Kaboratory "iagnostics. 1st ed. Fran7furt& (1#Ooo7s LerlagsgesellschaftN 1AA:. p. 1.1#3. Dac7s "O. <arbohydrates. >n& Ourtis <%, %shwood EB, editors. (iet' (extboo7 of <linical <hemistry. . rd ed. $hiladelphia& H.O Daunders <ompanyN 1AAA. p. 345# :5:. Oarham ", (rinder $. %n improved color reagent for the determination of blood glucose by the oxidase system. %nalyst 1A32NA3&1*2#4.

..

Method Comparison % comparison between "iasis !lucose FD +y- and a commercially available test +x- using 3: samples gave following results& y J 1.55 x / 1.55 mg6dlN rJ 5.AA@.

Manufacturer
"iasis "iagnosti7 Distemler (ic. Le Dan. %.P. Mer7e' Mah.Ourcu D7. Co&@6* Mart $la'a .**5@ Gagithane 9 >stanbul (8BGEQ (el &/A5+212- 2A* 25 55 Fax&/A5+212- 2A* 25 25

255*654 Bev & 55

!lucose !O" 9 $age 2