Code: 9A23505

B.Tech III Year I Semester (R09) Regular & Supplementary Examinations December/January 2013/14

PLANT BIOTECHNOLOGY
(Biotechnology)
Time: 3 hours Answer any FIVE questions All questions carry equal marks ***** 1 Discuss in detail about the roles of macro, micro nutrients, trace elements, amino acids in a plant tissue culture media. Discuss the need for germplasm conservation. List out various methods of cryopreservation for plant propagules. (tissues/ seeds/ embryos etc) Compare and contrast continuous culturing of plant cells with batch culturing with its merits and demerits. (a) Discuss in detail about co integrative vector strategy with suitable diagram. (b) Illustrate binary vector strategy with a commercial vector system to carry out plant transformation. Comment on -endotoxins. Discuss in detail about the methods to generate a Bt- resistant plant (brinjal). Comment on the acceptance pattern of the same in India and a developed country. How a generation of free radicals cause stress to plants. Discuss in detail about the roles of five important anti-oxidants that may be useful in reducing the effects of free radicals. Discuss in detail about producing a transgenic canola in improving the unsaturated fatty acid content by genetic modification. Discuss a case study using tissue culture to mass produce a commercially important drug (Ginseng). What strategies can be followed to increase its yield? Max. Marks: 70

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Code: 9A23505

B.Tech III Year I Semester (R09) Regular & Supplementary Examinations December/January 2013/14

PLANT BIOTECHNOLOGY
(Biotechnology)
Time: 3 hours Answer any FIVE questions All questions carry equal marks ***** 1 Describe in detail a case study separately for direct and indirect somatic embryogenesis, with suitable diagrams. Mention the advantages of using somatic embryos in tissue culture. Discuss in detail about anther/pollen culture for the production of di-haploid plants. What are the prospects and consequences of di-haploids? Examine in detail a case study in tissue culture, where you are asked to mass produce a plant secondary metabolite using root culturing. Discuss laboratory about direct gene transfer methods to other transgenic plants with its merits and demerits (at least 5 methods) Discuss in detail about the constructs, methods used to produce a genetically modified heritricide (glyphosate) tolerant cotton plants. Discuss the pros and cons of this transgenic strategy (Agro bacterium mediated method) Annual decrease in rainfall, Annual increase in temperature Justify the importance of drought tolerant plant with relevant to transgenic technology. Discuss a strategy to produce a stable transgenic plant (rice). What are PHBs? Explain in detail how commercially PHBs can be utilized from transgenic technology from plants. What are plant primary metabolites? How they differ from secondary metabolites? Discuss about any five important anti cancerous secondary metabolites. Max. Marks: 70

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Code: 9A23505

B.Tech III Year I Semester (R09) Regular & Supplementary Examinations December/January 2013/14

PLANT BIOTECHNOLOGY
(Biotechnology)
Time: 3 hours Answer any FIVE questions All questions carry equal marks ***** 1 (a) Discuss laboratory about protoplast culture. (b) Discuss about the practical importance of protoplast fusion with proven examples. How will you generate a mapping population in lesser time with a negatively higher homozygosity using a tissue culture technique? Discuss a commercial model bioreactor which can be used with case to produce vital drug/chemical from plant cells. (a) Discuss in details about CaMv, gemine virus, begamo viruses with its genome construct details and how it can be modified and used as plant transformation vectors. (b) What is agro infection? Discuss the advantages and disadvantages in using viral vectors for plant transformation. (a) Discuss in detail about PR proteins and its classification. (b) Discuss a case study of gene pyramiding (or) gene cascading as a method in plant transformation, making a plant to resist multiple diseases. Discuss in detail about the strategies and methods to develop a genetically modified plant to have multiple plant to have multiple a biotic stress tolerance. Design a gene construct, assume necessary conditions and illustrate the same. How plants can be engineered to produce therapeutically important proteins. Discuss with an example. Altering/modifying a metabolic pathway can influence other pathways drastically with the above condition, how one can design metabolic engineering to maximize the yield of secondary metabolite in plants. Max. Marks: 70

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Code: 9A23505

B.Tech III Year I Semester (R09) Regular & Supplementary Examinations December/January 2013/14

PLANT BIOTECHNOLOGY
(Biotechnology)
Time: 3 hours Answer any FIVE questions All questions carry equal marks ***** 1 (a) What is a callus? Describe in detail about callus culture, its types, how one can make a compact callus friable? (b) Describe in detail about suspension culture. How suspension culture is superior to other types of cultures? Describe in detail about the causes for soma clonal variations. How one can avoid producing soma clonal variants in plant tissue culture. Discuss the demerits of somaclonal variations. Discuss a case study where a specific processes are involved in eliciting/enhancing maximum production of pharmaceutically important secondary metabolite. Discuss in detail about the roles of various proteins and the steps involved in transfer and integration of the gene of interest inside a plant cell by Agrobacterium mediated transformation. Discuss in detail about the usage of cross protection in plants to generate a virus resistance. With a specific case study and other examples. (a) Describe the mechanism involved in preventing protein denaturation due to the presence of osmoprotectants in higher salinity condition. (b) Discuss the methods and strategies to produce a saline tolerant rice by genetic modification with construct details. What are plant bodies? Discuss how plants can be used as a bioreactors to produce edible vaccines. Discuss the merits and demerits of plant vaccines with examples. Discuss the case study about the alteration of metabolic pathway (isoprenoid pathway) to produce dwarfism in tomato plants with higher carotenoid content. Max. Marks: 70

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