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    Cloning the maize heat shock protein into the vector pGAL. You need to choose the restriction enzymes that you will incorporate into your PCR primers and that you will use to cut the vector DNA.

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    Cloning the maize heat shock protein into the vector pGAL. You need to choose the restriction enzymes that you will incorporate into your PCR primers and that you will use to cut the vector DNA.

    Hak Cipta:

    Attribution Non-Commercial (BY-NC)
    Unduh sebagai PDF, TXT atau baca online dari Scribd
    Tandai sebagai konten tidak pantas
    251 tayangan1 halaman

    08Q PDF

    Diunggah oleh

    FCiências
    Cloning the maize heat shock protein into the vector pGAL. You need to choose the restriction enzymes that you will incorporate into your PCR primers and that you will use to cut the vector DNA.

    Hak Cipta:

    Attribution Non-Commercial (BY-NC)
    Unduh sebagai PDF, TXT atau baca online dari Scribd
    Tandai sebagai konten tidak pantas
    dari 1

    Question 8

    You are interested in cloning the maize (corn) heat shock protein into the vector pGAL. You need to choose the restriction enzymes that you will incorporate into your PCR primers and that you will use to cut the vector (pGAL) DNA. The following are maps of the multiple cloning site of pGAL and the DNA sequence that encodes the maize heat shock protein.

    Another student in the lab suggests that you clone the maize gene by including an XbaI site in your forward primer and an SspI site in your reverse primer. Do you agree or disagree with this strategy? Provide two reasons for your answer.

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