Test: ELISA | Quizlet

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ELISA

NAME: ________________________

F
9 Multiple Choice Questions
1. Competitive/Inhibition ELISA

0%

(No Answer)
a. - coating bu!er - prevents interaction between plastic and antibodies b. - horseradish peroxidase - secondary antibody - amplier c. CORRECT: - can use any of previous 3 setups - Quantitative - 1st Case sample is added, 2nd primary Ab added, washed, Active Ab added, washed, substrate added - Result: if positive color change -> case sample doesn't bind - Result: if negative color change -> case sample binds d. - contains antigen, antibody/enzyme complex, blocker, and substrate - reverse is uncommon - faster, less error prone - reduced sensitivity 2. PBS

(No Answer)
a. - horseradish peroxidase - secondary antibody - amplier b. - matched Ab's - highly specic - exible (Direct or indirect) - Ag DOES NOT NEED TO BE PURIFIED - indirect not usually done, only di!erence is that detecting antibody does not directly catalyzes substrate c. CORRECT: - coating bu!er - prevents interaction between plastic and antibodies d. - primary antibody

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Test: ELISA | Quizlet

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3. TMB

(No Answer)
a. - matched Ab's - highly specic - exible (Direct or indirect) - Ag DOES NOT NEED TO BE PURIFIED - indirect not usually done, only di!erence is that detecting antibody does not directly catalyzes substrate b. CORRECT: - 3,3',5,5'-tetramethylbezidine - hydrogen peroxide - stabilizing bu!er - blue when reduced - amount of blue is proportional to amount of antibody c. - horseradish peroxidase - secondary antibody - amplier d. - can use any of previous 3 setups - Quantitative - 1st Case sample is added, 2nd primary Ab added, washed, Active Ab added, washed, substrate added - Result: if positive color change -> case sample doesn't bind - Result: if negative color change -> case sample binds 4. Simple direct ELISA

(No Answer)
a. - horseradish peroxidase - secondary antibody - amplier b. - can use any of previous 3 setups - Quantitative - 1st Case sample is added, 2nd primary Ab added, washed, Active Ab added, washed, substrate added - Result: if positive color change -> case sample doesn't bind - Result: if negative color change -> case sample binds c. CORRECT: - contains antigen, antibody/enzyme complex, blocker, and substrate - reverse is uncommon - faster, less error prone - reduced sensitivity d. - antigen - coats the bottom of the ask - searves as an attachment point for primary antibody

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5. Sandwich ELISA

(No Answer)
a. CORRECT: - matched Ab's - highly specic - exible (Direct or indirect) - Ag DOES NOT NEED TO BE PURIFIED - indirect not usually done, only di!erence is that detecting antibody does not directly catalyzes substrate b. - horseradish peroxidase - secondary antibody - amplier c. - coating bu!er - prevents interaction between plastic and antibodies d. - 3,3',5,5'-tetramethylbezidine - hydrogen peroxide - stabilizing bu!er - blue when reduced - amount of blue is proportional to amount of antibody 6. Ovalbumin

(No Answer)
a. - horseradish peroxidase - secondary antibody - amplier b. - coating bu!er - prevents interaction between plastic and antibodies c. CORRECT: - antigen - coats the bottom of the ask - searves as an attachment point for primary antibody d. - can use any of previous 3 setups - Quantitative - 1st Case sample is added, 2nd primary Ab added, washed, Active Ab added, washed, substrate added - Result: if positive color change -> case sample doesn't bind - Result: if negative color change -> case sample binds

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Test: ELISA | Quizlet

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7. Elisa

(No Answer)
a. - can use any of previous 3 setups - Quantitative - 1st Case sample is added, 2nd primary Ab added, washed, Active Ab added, washed, substrate added - Result: if positive color change -> case sample doesn't bind - Result: if negative color change -> case sample binds b. - coating bu!er - prevents interaction between plastic and antibodies c. CORRECT: - enzyme-linked immunosorbant assay - attach either antibody or antigen to bottom of plastic well - detects presence of Ab or Ag; can be gualitative or guantitative - colorimetric method d. - horseradish peroxidase - secondary antibody - amplier 8. Rabbit Anti-Ovalbumin

(No Answer)
a. - horseradish peroxidase - secondary antibody - amplier b. CORRECT: - primary antibody c. - enzyme-linked immunosorbant assay - attach either antibody or antigen to bottom of plastic well - detects presence of Ab or Ag; can be gualitative or guantitative - colorimetric method d. - coating bu!er - prevents interaction between plastic and antibodies 9. HRP

(No Answer)
a. CORRECT: - horseradish peroxidase - secondary antibody - amplier b. - primary antibody c. - can use any of previous 3 setups - Quantitative - 1st Case sample is added, 2nd primary Ab added, washed, Active Ab added, washed, substrate added - Result: if positive color change -> case sample doesn't bind - Result: if negative color change -> case sample binds d. - coating bu!er - prevents interaction between plastic and antibodies

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07/02/14 8:55 PM