BIY201 Version No.: 1.

10

Molecular Biology

L T PC 3 0 4 5

Course Prerequisites : BIY102 Fundamental Biochemistry Objectives: to prepare the student to gain knowledge on molecular mechanism of propagation and gene expression. Expected Outcome: Students should be able to understand the principles of molecular biology and apply in the field of genetic engineering, medicine, and understanding of life processes. Genetic Material Unit No. 1 9 hours Concept and organization of genetic material. Physical and chemical structure of DNA, denaturation and re-naturation kinetics of DNA. Genome organization in prokaryotes and in eukaryotes. DNA packaging in nucleosome, chromatin and chromosome. Identification of dna as genetic material. Central dogma concept. Unit No. 2 DNA replication 9 hours Semi-conservative replication. Enzymes in DNA replication-prokaryotic dna polymerases and eukaryotic DNA polymerases, fidelity and precessivity of polymerases. Replication in prokaryotes-origin of replication, fork formation, complex replication apparatus, replication of leading and lagging strand. Okazaki fragments. Elongation, termination of replication. Eukaryotic DNA replication. Inhibitors of replication. Unit No. 3 Transcription 9 hours RNA structure, types of RNA, Gene Structure, RNA polymerases, transcription in prokaryotesinitiation and elongation, promoters, termination of transcription. Eukaryotic promoters. Distinction between pro and eukaryotic transcription. Posttranscriptional processing and modifications of rna-mRNA, t-RNA and r-RNA, rverse transcription. Unit No. 4 Translation 9 hours Protien structure-folding of polypeptide chain, alpha helix and beta secondary structures. Genetic code and its feature, wobble hypothesis, uniniversality of genetic code. Translation initiation, elongation and termination in prokaryotes. Translation in eukaryotes. Post translational modifications. Antibiotics-inhibitors of protein synthesis. Unit No. 5 Regulation of gene expression 9 hours Principles of regulation. Cis acting sites and trans acting molecules, transcriptional regulation, Transcription factor, DNA response element, feedback inhibition and allosteric control. the lac operon, the trp operon, regulation of m-RNA stability, regulation of translation, eukaryotic regulation. Role played by HATs, HDACs, methylases and demethylases in regulation of gene expression. Textbooks 1. Freifelders essentials of molecular biology by George M. Malacinski, Narosa Pub 2005. 2. Fundamentals of Molecular Biology by Jayant K Pal and Saroj S Ghaskadbi, 2009, Oxford Press, India.

Proceedings of the 29th Academic Council [26.4.2013]

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Reference Book: 1. Molecular biology of gene by Watson et. 2. Genes VIII by Benjamin Lewin, Oxford univ. Press. Mode of Evaluation: Continuous Assessment (Quizzes, CATs, Assignments, etc.) and TEE

Experiments 1. Preparation of common buffers used in molecular biology. 2. DNA isolation from Bacteria, animal and plant tissue. 3. Analysis of isolated DNA on agarose gel electrophoresis 4. RNA isolation from Bacteria, animal and plant tissue. 5. Analysis of isolated RNA on MOPS agarose gel electrophoresis 6. Protein isolation from Bacteria, animal and plant tissue. 7. Protein estimation 8. Separation of proteins by SDS PAGE 9. Western blotting

Mode of Evaluation:

Laboratory Examinations, Viva - Voce

Proceedings of the 29th Academic Council [26.4.2013]

656