0ui stianu of BNA:

!" $ % & ' % ("

(" % $ ' & $ !"

If we want to get oui RNA sequence, we must tiansciibe the BNA. Remembei, the
cential uogma is

)*$ ->tiansciiption-> +*$ ->tianslation-> ,-./012

Buiing tiansciiption, only one of the BNA stianus is actually useu by the RNA
polymeiase as a template. The othei stianu, the so-calleu "couing" stianu, is not
useu uuiing tiansciiption. Because it is the ieveise complement of the template
stianu, the 3.4125 6/-724 will have /80 0973/ 67:0 60;<0230 724
41-03/1.27=1/> 76 /80 +*$ /-7263-1?/@

What uoes this look like uuiing tiansciiption. Let's go back to oui stianu of BNA.

!" $ % & ' % (" AB 3.4125
(" % $ ' & $ !" AB /0:?=7/0

In this example, we will tieat the top stianu as the couing stianu anu the bottom
stianu as the template stianu. To tiansciibe oui RNA, we can use eithei stianu:

0sing the C0BINu stianu:

!" $ % & ' % (" AB 3.4125

Beie oui RNA is the same sequence anu uiiectionality, only with T's ieplaceu with
0's, iesulting in the following RNA:

!" $ % & ' % ("

If we ueciue to use the TENPLATE stianu insteau:

The /0:?=7/0 6/-724 is the -0C0-60 3.:?=0:02/ D-0C0-60
41-03/1.27=1/>E 3.:?=0:02/ .F /80 G7606H .F /80 +*$ /-7263-1?/@

(" % $ ' & $ !" AB /0:?=7/0


0ui RNA shoulu look like this:

!" $ % & ' % ("

As you can see, iegaiuless of which stianu you use, as long as you follow the coiiect
iules you shoulu get the same stianu of RNA eithei way. Foi assignments anu
exams, $IJ$KL %+M$% %NM L%+$*) J+O%%M* !" BP (" $L %NM &Q)O*'
L%+$*)@

To ieview:

Couing stianu = same uiiection, same sequence as RNA
Template stianu = ieveise uiiection, complement sequence of RNA

A seconu piece of BNA:

!" $ % ' & $ % % $ ' ("
(" % $ & ' % $ $ % & !"

Answei the following questions:

1. Which stianu is the couing stianu. Which is the template stianu.

2. Tiansciibe the RNA using the couing stianu. Tiansciibe the RNA
using the template stianu. Is youi answei the same.

S. Tianslate youi RNA.

4. What will happen if we take the ieveise complement of oui couing
stianu. What is oui piotein sequence insteau. Is it uiffeient fiom youi
answei foi #S.

1 ) T o p s t i a n u i s c o u i n g , b o t t o m s t i a n u i s t e m p l a t e . 2 ) R N A s e q u e n c e s h o u l u b e S ' A 0 u C A 0 0 A u S ' . S ) N E T - B I S - S T 0 P . 4 )
R N A s e q u e n c e i s S ' C 0 A A 0 u C A 0 S ' , p i o t e i n i s L E 0 - N E T - B I S . T h e s e q u e n c e s a i e u i f f e i e n t .