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FHSB1214 BIOLOGY I FOUNDATION IN SCIENCE Name : ANGELENE A/P MUTHU SELWANATHAN Partner’s name : SOPHIA

FHSB1214 BIOLOGY I

FOUNDATION IN SCIENCE

FHSB1214 BIOLOGY I FOUNDATION IN SCIENCE Name : ANGELENE A/P MUTHU SELWANATHAN Partner’s name : SOPHIA
Name : ANGELENE A/P MUTHU SELWANATHAN Partner’s name : SOPHIA NOVALINA R GUK GUK Class: S
Name :
ANGELENE A/P MUTHU SELWANATHAN
Partner’s name :
SOPHIA NOVALINA R GUK GUK
Class:
S stream
Date of lab class :
Program :
Unit code :
Tuesday , 25 th February 2014
Foundation in science.
FHSC 1214
Unit description :
Fundamentals of Cell Biology
Year and trimester
of study:
2014, trimester 1
Title of lab report:
Practical 4 Investigation of the Enzymatic Effects of
Materials on Hydrogen Peroxide
Lecturer’s name:
Miss.Nicole Ngai Suet Loo

Marking Scheme

Investigation of the Enzymatic Effects of Materials on Hydrogen Peroxide Solution

Objective: To investigate the enzymatic effect of various materials in the hydrogen peroxide solution

Final score (rounded to nearest integer): 6/6

The final score for this assignment is based on the average of scores ranked as follows:

1: poor; 2: lacking: 3: good; 4: very good; 5: excellent; 6: perfect

Comments are indicated by „

>>>
>>>

‟ (in detail), „

???
???

‟ (unclear) or „

xxx ‟ (wrong concept)

xxx‟ (wrong concept)

FHSB1214 Biology I (201301)-Lab Report Marking Scheme (Practical 6)

 

Item

Description

Marks

Format

Correct file format (.doc/.docx), correct file

/1

name (student‟s name)

 

Result

Observation obtained using wooden splint for each test tube (effervescence/ bubbles formed / wooden splint / pop sound)

/6

Discussion

Answers based on marking scheme

/11

Total

 

/18

Title: Investigation of the Enzymatic Effects of Materials on Hydrogen Peroxide

Solution.

Objective: To investigates the enzymatic effect of various materials in the hydrogen peroxide solution.

Results:

Test

Contents with 5 cm3

Observation before and after using wooden splinter

Tube

of hydrogen peroxide

Before

 

After

       

Reaction on

Sound that is

Presence of

 

wooden

produced

white fumes

splint

 

Fresh liver

  • 1. High amount

Wooden

None

Present

of bubbles is

splint ignites

produced.

with an

   

orange

flame.

 

Boiled liver (cooled)

  • 2. Bubbles are

 

Wooden

None

Present

produced.

splint

remains the

same.

 
  • 3. Effervescence

Pulped liver

occurs.

Wooden splint ignites with a yellow flame.

None

Present

 
  • 4. Bubbles are

Potato cubes

 

Wooden

None

Present

produced.

splint

remains the

same.

 
  • 5. Effervescence

Manganese dioxide

 

Wooden

 

Present

(untreated)

occurs.

splint ignites

“pop” sound

with bright

is produced

yellow flame.

 
  • 6. Effervescence

Boiled Manganese

 

Wooden

 

Present

dioxide (cooled after

occurs.

splint ignites

“pop” sound

heating )

with a bright red flame.

is produced

Discussion:-

Hydrogen peroxide is a metabolic by-product of chemical reaction that‟s produced in liver. Hydrogen peroxide can be deadly if it has been accumulated too much in body, so it has to be decomposed. One of the decomposing factors is the enzyme catalase. Catalase breaks hydrogen peroxide into water and oxygen. The equation that was formed based on the observations are such that:

2(H2O2)

2(H2O2) 2H2O +O2.When involving manganese dioxide the equation formed is 2H2O2

2H2O +O2.When involving manganese dioxide the equation formed is 2H2O2

+ MNO2

2H2O + O2, hence the product formed is water and oxygen cause for both

reaction because manganese does not take part in the reaction. It only acts as a catalyst which speeds up the reaction. The plant and animal organelle that was involved is peroxisome, an organelle which contains the enzyme catalase which decomposes hydrogen peroxide.

When the liver has been pulped, the reaction is more vigorous due to the increased surface

area for the enzyme to act on. Hence, larger the surface area, more exposed it is to the reactant. Meanwhile, the boiling of the liver produces less result because enzyme has been denatured due to high temperature. This because enzyme is protein and it is held by weak

bonds and the molecular motion changes. This different molecular motion alters the enzyme‟s

shape and makes it to lose its ability to bind with other substrate due to the enzyme is not

complement to its active site. To compare the difference of reaction between fresh liver and

potato, the fresh liver has produced more bubbles compared to potato which indicates that the liver has more catalase activity than in potato. This is due to the different function of liver and potato in nature. Catalase in liver aids for detoxifying chemicals whereas catalase in potato prevents the oxidation of starch. The boiling of liver and heating the manganese oxide produces different kind of results though in both the tubes, heat were added to it. When boiling a liver, the enzyme is denatured due to high temperature as enzyme is protein and has proteins‟ characteristic. Protein characteristic which involved here is the high sensitivity towards heat because high temperature denatures the proteins‟ structure and makes it to lose its ability to bind to the active site of the product because it‟s no longer complementary.

Whereas, boiling the manganese oxide eventually activates it because it is an inorganic catalyst, thus it does not denature on heating .Presence of white fumes, the pop sound and

wooden splinter igniting indicates strongly that there‟s presence of oxygen gas based on the

observation.