ISSN 0975-2722

Vol. XXX, No. 1-2 Hkkx&30] vad 1&2

January-June, 2009 tuojh&twu] 2009




Journal of Drug Research in Ayurveda and Siddha
=.| || '== =..'. ===..= |'=.







Published by
CENTRAL COUNCIL FOR RESEARCH IN AYURVEDA AND SIDDHA
NEW DELHI

Editorial and Business Office:
Jawahar Lal Nehru Bhartiya Chikitsa Evam
Homoeopathy Anusandhan Bhawan
Institutional Area, No. 61-65
Opp. D-Block, Janakpuri
New Delhi 110058

Annual Subscription In India Rs. 120/-; Abroad US $ 40

Journal of Drug Research in Ayurveda and Siddha


Vol. XXX, No.1-2
January-June, 2009




PATRON
Union Minister for Health & Family Welfare
(Government of India)




Editor-in-Chief
Prof. G.S. Lavekar



Editor
Dr. M.M. Padhi



Editorial Board
Dr. S.N. Gaidhani
Dr. G.V.R. Joseph
Dr. B.P. Dhar
Dr. Prameela Pant
Dr. Neera Vyas
Dr. M.M. Sharma

JOURNAL OF DRUG RESEARCH IN AYURVEDA AND SIDDHA

EDITORIAL ADVISORY BOARD

Dr. Lohar Dr. (Mrs.) Veena Gupta
Dr. Raman Singh Dr. S.H. Parihar
Dr. Shamshad Ahmed Dr. B.M. Singh
Dr. Y.K.S. Rathore Dr. S. Rajasekharan
Dr. K.S. Nagaraja Prof. P. Das
Dr. S. Srivastava Dr. Manisha Sarkar
Dr. Bikram Singh Dr. V.K. Lal
Dr. Naveen Khare Dr. (Mrs.) Shanta Mehrotra
Dr. Suresh Walia Dr. Rajeev Sharma
Prof. V.K. Kapoor Dr. Roma Mitra
Dr. H.B. Singh Dr. S.D. Kamat
Dr. V.K. Singh Dr. S.J.S. Flora
Dr. K.C. Bhatt Dr. Y.P. Sahni
Dr. K.S. Negi Dr. K.K. Srivastava


Editorial Support
Mr. N.K. Gupta Mr. Upendra Singh Mr. Syed Fahar Ali

JOURNAL OF DRUG RESEARCH Vol. XXX, No.1-2
IN AYURVEDA AND SIDDHA January-June, 2009



CONTENTS

S.No. Particular

Page No.
1. Evaluation of in vivo anthelmintic efficacy of Swertia chirata
(Chirayata) against Gastrointestinal Nematodiasis in Goats
Manpreet Arora, Y.P. Sahni, Vidhi Gautam and Alok Singh

1-8
2. Ethno-Medicinal studies on plants used in the treatment
of Urino-genital Disease by Bhil Tribe of Ratlam District (M.P.)
Dinesh Jadhav

9-18
3. Hepatoprotective Activity of Withania somnifera Dunal
(Ashwagandha) on Paracetamol induced liver damage in
Albino Rats
Y.P. Sahani and Vidhi Gautam

19-26
4. Pharmacognostical study of roots of Merremia emarginata
(Burm. F.) Hall. F. and Merremia aegyptia (Linn.) Urban
Suman Kumari, V.J. Shukla and Alka Agarwal

27-38
5. Effect of Decoction of Butea monosperma (Linn.) Kuntze
and Daucus carota (Linn.) Var. sativa seeds on oestrous cycles
of Female Albino Rats
Shilpa Donga, Vinay J. Shukla, Sarada Ota, B. Ravishankar
And I.U. Mistry

39-48
6. Development of standard operating Procedure
and Pharmacopoeial standards for Raca Kanti Meluka (RKM)
A Siddha Formulation
A. Saraswathy, Arunmozhi Devi and Nandhini Devi
49-58

EVALUATION OF I N VI VO ANTHELMINTIC
EFFICACY OF SWERTI A CHI RATA
(CHIRAYATA AGAINST GASTROINTESTINAL
NEMATODIASIS IN GOATS

Manpreet Arora
1
, Y.P. Sahni
2
, Vidhi Gautam
3
and Alok Singh
4


(Received on 14.12.2008)

Investigations were conducted to evaluate in vivo anthelmintic efficacy of aqueous extract of
seeds of Swertia chirata (Chirayata) against some of the most common pathogenic gastrointestinal
nematodes such as Haemonchus, Bunostomum and Oesophagostomum spp. in naturally infected
goats. The aqueous extract was administered at the dose of 550 mg/kg body weight, orally for 10
consecutive days in adult Goats naturally infected with gastrointestinal nematodes. The
anthelmintic activity of S. chirata was compared with Albendazole (5 mg/kg, oral), a standard
anthelmintic drug used in clinical practice. The anthelmintic efficacy of aqueous extract was
assessed by determining the mean EPG count on day 0, day 3, day 6, day 9 and day 12
respectively. Examination of the fecal samples revealed significant and progressive reduction in
the number of eggs in feces (EPG) from second post treatment day onwards and till day 12. The
anthelmintic efficacy based on fecal scores in Goats treated with S. chirata and Albendazole on
day 12 of the experiment was found to be 62.50% and 100%. The findings indicated that S. chirata
exhibited significant in vivo anthelmintic efficacy against gastrointestinal nematodes in Goats.



1

& 4
M.V. Sc. Student
2
Associate Professor
3
Asst. Professor Dept. of Pharmacology & Toxicology, College of Veterinary Science & Animal Husbandry to
Jabalpur

=...
'=.+. = ===| =. ='. = =.+='= |
|=.|. =..= ='=+. =. ==
==|.+ =.., |. |.. =.r=., '|'. .+= || =.=.= '=r

'=.+. = .=. = ===| =. |... ='. = =....+ |. =.= |.= =.+='= ==r.=.-==,
=.-.== +.. ;=.=.-. == = '|.. = |=.|. =..= ='=+. =. =.== = '=| |.='+= =| = |..'|+
='. = '=. . r '=.+. = ===| =. ssc '=..=.'==..=.=.=| =. = = '= += =.+.
'. . ===| =. |=.|. =..= .=+. Mean EPG = .. =.. '=, +.= '=, =.| '= | .r| '=
| '=..'+ =. ; ='. = == = |... .. r '== r=. '= '=+. = ===| =. = = = '=
= r. |=.|. = =. =. =. = ==. =.=. .= r. ; '=+. = ===| =. |=.|. =..= .=+. =. ==
|... -=. ='. = .r| '= = '='==. .|.+ ez.sc |'+.+ r. ='= ==-.=.= =. (s '=.
.=.'==..=.=.=|) |=.|. =..= .=+. =. == |... -=. cc |'+.+ r.
ETHNO-MEDICINAL STUDIES ON PLANTS USED
IN THE TREATMENT OF URINO-GENITAL
DISEASE BY BHIL TRIBE OF
RATLAM DISTRICT (M.P.)

Dinesh Jadhav
1


(Received on 17.12.2008)

Frequent ethnomedicinal surveys were conducted during August 2004 to March 2007 in
different tribal inhabited villages of Ratlam District (M.P.). Result of these surveys indicated that 34
plant species are interestingly used for the treatment of urinogenital diseases prevalent in the Bhil
tribe of the district. Botanical names followed by family, local names and the ethnomedicinal uses
have been provided.

1
30, Rajiv (Kasturba) Nagar, Ratlam 457001 (M.P.) INDIA
=...
+=.= ==| =. ..= ===.'+ .. =|= = || ==='- =..
.. = .|=. = =. =.= |.= |.|. =. =.=|=.... ==

'=. =..|

+=.= ==| = '|'.-= . |. =. =-+ zcc+ = =.= zccz = = |= =.= |=.... =|.. '=.
. ;== |'..=-|=| -.+ r=. '= ;= ==| = '=|.= == |.=. ..= ===.'+ ++ |.| |=.'+. =.
=|=. = || ==='- =.. '|'.-= .'.. =. '='==. = .== +.= = |. =+. r ;= || = ;=
++ |.| |=.'+. = |.=-|'+= =.= = =.. r. == = =.=, -..=. =.= | =.=.... .|. ' r
HEPATOPROTECTIVE ACTIVITY OF WI THANI A
SOMNI FERA DUNAL (ASHWAGANDHA) ON
PARACETAMOL INDUCED LIVER
DAMAGE IN ALBINO RATS

Y.P. Sahani
1
and Vidhi Gautam
2


(Received on 24.12.2008)

In the present study, aqueous and alcoholic extracts of Withania somnifera were screened for
their hepatoprotective activity on paracetamol induced liver damage in albino rats. The percent
hepatoprotective activity of Withania somnifera, in terms of three major enzymes of liver viz.
SGOT, SGPT and ALP was found to be 91.30%, 77.77% and 65.4% for alcoholic extract and
74.08%, 68.92% and 53.75% for aqueous extract, respectively. The study showed that alcoholic
extract of Withania somnifera was more effective than alcoholic extract but its efficacy was found
to be lesser than standard hepatoprotective drug LIV-52.

1
Professor, College of Veterinary Science & Animal Husbandry, Jabalpur (M.P.) 482001
2
Asstt. Professor, College of Veterinary Science & Animal Husbandry, Jabalpur (M.P.) 482001
=...
|.=.=.= .. =+ =..= |..| =. =|.. .. =+ =.|
='=+. =. =r. | ==

|.. |.. =.r=. || '|'. .+=

|-++ == =, =|.. = ===| | |-=.r= =| =. |.=.=.= .. =+ =.. r.= | =+
=.| =. |... =r. | '=. . r =|.. =. =+ =.| ='=+. =. ==, =+ = |=.;= =
|'+.+ .. '=. . r =+ = +.= |=.;= |=.=..=...., |=.=..|.... +.. |.|=.|.. =. =+
=.| |'+.+ |-=.r= =| = 91.30%, 77.77% +.. 65.46% || ===| = 74.08%, 68.92% +..
53.75% |.. . ;= |.... = r |= r=. '= =|.. =. |-=.r= =|, ;== ===| = ='.=
=+ =.| = |..|=.. r


PHARMACOGNOSTICAL STUDY OF ROOTS OF
MERREMI A EMARGI NATA (BURM. F.) HALL. F.
AND MERREMI A AEGYPTI A (LINN.) URBAN

Suman Kumari
1
, V.J. Shukla
2
and Alka Agarwal
3


(Received on 13.01.2009)

Merremia is one of the important genus of family Convolvulaceae and this family is known
for the presence of glycosidal resin and its purgative property. Merremia aegyptia (Linn.) Urban
and Merremia emarginata (Burm. F.) Hall. F. are the two plants of the same genus belonging to
this family. The microscopic characters of Merremia aegyptia roots showed thick walled cork
cells, small clusters of rosette calcium oxalate crystals and slightly larger xylem vessels than
Merremia emarginata. The characteristic features of Merremia emarginata roots were one of two
layers of suberized cork cells and uni or bi seriate medullary rays. The cells of cortical region were
slightly polygonal to wavy in shape in Merremia aegyptia, while in Merremia emarginata
parenchymatous cells of cortex region were polygonal to spherical in shape.
TLF profile of Merremia aegyptia showed more intense and significant spots in selected
solvent system. Both the plant extracts showed many common spots having similar R
1
and colour
indicating more or less similar constituents but with different composition.

1
Senior Research Fellow (Chemistry), CCRAS, Janakpuri, New Delhi - 110058
2
Research Officer (Chemistry), CCRAS, Janakpuri, New Delhi - 110058
3
Co-opted Lecturer (Phytochemistry), IAMPS, Gujarat Ayurved University, Jamnagar, Gujarat
=...
=''==. ;='==.. (= |=.) r.=. =''==. ;'='=. ('=-=)
== = == =. ..= ='.-.=. ==

=====.., |..=. .+=. || ===. =|.=

=''=. =.-|.-|==. |'|. =. |= =r||. |. r =. r |'|. =.;=.'== '= || ...=
=|'. = '=| =.=. =.+. r =''=. ;'='=. ('=-=) == || =''=. ;='==.. (= |=.) r.=. |=.
;=. |'|. = |= |. = =. .+ r ==.. == = =''=. ;'='=. |.| = == =''=.
;='==.. =. +==. =.= .|. = '.. r; =.= =.'.=.=. =='.= =.+== '='-= = =. ==r ||
.=++ |. |'.+ =+ r |= . . =+r =. =|=.=. =.= =.'.=.| =. |=. || .|'++ ==.
'=. =''=. ;='==.. =. '|. '|..+. r =''==. ;'='=. = |.+-.. . =. =.'.=.| == r=.. =
+.+ =.=. =. r.+. r, .=.=. =.='+ =. r.+. r
=''=. ;'='=. ('=-=) == =. . |==. =| .. = ='.= || =r||. '=..= ..= .. |.
.=. |.|. = == = == ==.= =..|=. || = ==+ ' '= |= r. =.= . =|| =.
|'.+ =+. r
EFFECT OF DECOCTION OF BUTEA MONOSPERMA
(LINN.) KUNTZE AND DAUCUS CAROTA (LINN.)
VAR. SATIVA SEEDS ON OESTROUS CYCLES OF
FEMALE ALBINO RATS

Shilpa Donga
1
, Vinay J. Shukla
2
, Sarada Ota
3
,
B. Ravishankar
4
, And I.U. Mistry
5


(Received on 23.03.2009)

The anti-ovulatory activity of decoction of Butea monosperma (Linn.) Kuntze (Bacaceae)
seeds and Daucus carota (Linn.) Var. sativa (Umbelliferae) seeds were studied in rats. The
decoctions were administered to animals orally for 28 days. The vaginal smears were examined
daily for the number and duration of oestrous and di-oestrous cycles. The oestrous phase was
reduced while the di-oestrous phase was persistent and prolonged in both the treatment groups,
thus revealing anti-fertility potential of these herbs.

1

& 5
Lecturer, Deptt. of Streeroga & Prasutitantra

2
C-opted Lecturer
3
Research Officer (Ayurveda), CCRAS, New Delhi - 110058
4
Head in I.M.P.S., Deptt.of Pharmacology, I.P.G.T. & R.A., Gujarat Ayurved University, Jamnagar, Gujarat

=...
|=...= +|.. || .= .= +|.. =. =.. =r. = ;- = ==
| ==

|=. .. .., |.. =. .+=, |=. =.., .. '| .= || =.;. . '=-.

|-++ ...| = |=.. .= +|.. || .= .= +|.. =. (. '=..=) |-.=.=. == .=
= '= =.. =r. = ;-= == =. == '=. . r |=...= +|.. ezc '=..'==..= .. ..
+.. .= .= +|.. zec '=..'==..= .. .. = z '= += = | ;-= '=. = =r||. ==.
|.; ;, ='= r.;;-= = |'= || -...| |.. . ;= |=. =.=.= =. .= = .'=..= =| =
.|.. r. ==+. r
DEVELOPMENT OF STANDARD OPERATING
PROCEDURE AND PHARMACOPOEIAL STANDARDS
FOR RACA KANTI MELUKA (RKM)
A SIDDHA FORMULATION

A. Saraswathy
1
, Arunmozhi Devi
2
and Nandhini Devi
3


(Received on 29.09.2009)

Siddha medicinal system is an ancient system, practiced for over 5000 years. Siddha
experts indicate that Siddha formulations have been proved to be useful in controlling venereal
disease (VD) including diseases closely resembling to HIV clinically. The drugs like Raca Kanti
Meluka (RKM), Amukkara curanam (AC), Nellikkai ilakam (NI) and in combination of these
drugs were reported to be useful in HIV patients for both clinical improvement and elevation of
CD
4
counts. In the present study, an attempt has been made to develop Standard Operating
Procedure for the preparation and Pharmacopoeial standards for Raca Kanti Meluku. The
developed SOP and analytical standards can be used for fixing standards in Siddha
Pharmacopoeia.

1
Assistant Director In-charge
2

& 3
Senior Research Fellows, Captain Srinivasa Murti Drug Research Institute for Ayurveda and Siddha (CCRAS),
Arumbakkam, Chennai - 600106


=...
= =' ===|= '== =... . = '=| =.== |=.==
=.'|'.. || ..==... =.==. =. '|=.=

|. =-|+., ==.=.=. |. || =-=.|.

'== '='==. |='+ |= |.=.= '='==. |='+ r r '='==. |='+ =. sccc |.. = |. =. =.
r. r '== '|..- r ='=+ =+ r '= |= = =.. '==+ ==+ =.. .. = ='r+ '+= .. =
'=. = '== =... .. =. |. ='.+ |.. . r '== =... = =' === (=.=|=), ==+=.
=.= (|=.), ='-==. ;=+== (|==.;) =. ;== =... =.==. =. |. |= =.; |. .'. = '=|
.|.. |.. . r ;= =.... = .. =.'== =.. || =. . + .=. = ...= r=. |-++ ===..=
=, = =' === =... =. +.. r+ =.== |=.== =.'|'.. =. ..==.'. =.==. =. '|='=+ == =
'=| |.= '=| | r '== ..= =... =.==. = '=... = ;= ===..= = .. '|='=+ |= =.|. ||
'|='.+ =.==. =. .|. '=. =. ==+. r