B2."f: #ecogni$e and describe that both li!ing and nonli!ing things are composed of compounds, %hich are themsel!es made up of elements joined by energy&containing bonds, such as those in '(). '2*7 iving (rganism Composition All living or once8living organisms are composed o) car!ohydrates, lipids, proteins, and n&cleic acids* Car!ohydrates and lipids contain many car!on8hydrogen !onds that also store energy '2*7x Energy +rans)er All living or once living organisms are composed o) car!ohydrates, lipids, proteins, and n&cleic acids* Car!ohydrates and lipids contain many car!on8hydrogen !onds that also store energy* Ho,ever, that energy m&st !e trans)erred to A+# 3adenosine triphosphate4 to !e &sa!le !y the cell* '2*7A: 5ecognize and explain that macromolec&les s&ch as lipids contain high energy !onds* 5et&rn to 9nit : +a!le o) Contents
5et&rn to 9nit : +a!le o) Contents +ipids are made by the process of dehydration synthesis Di))erent -inds o) )ats: Single !onds D sat&rated )ats 3&s&ally solids )rom animals s&ch as !&tter4 Do&!le or triple !onds D &nsat&rated )ats 3&s&ally li2&ids )rom plants s&ch as corn and vegeta!le oils4 Atherosclerosis is a condition ca&sed !y too m&ch sat&rated )at in yo&r diet ca&sing lipid deposits 3pla2&e4 to acc&m&late in yo&r !lood vessels, red&cing !lood )lo,* (rans fats are unsaturated fats con!erted to saturated by adding hydrogens. Example: &s&ally a so)tened solid s&ch as margarine* +rans )ats &sed to !e called hydrogenated )ats* #hospholipids, ,axes, and steroids are lipids ,ith a variety o) )&nctions #hospholipids 8 str&ct&rally similar to )ats !&t contain phosphor&s ? 2 )atty acids 3not "4 are &sed mainly in mem!ranes o) the cell* ;axes , one )atty acid lin-ed to an alcohol 3coating )or )r&its E insects have ,axy coats to prevent dehydration4* Steroids D car!ons )orm rings s&ch as in the case o) cholesterol 3also )o&nd in cell mem!ranes4* )roteins #roteins are essential to the str&ct&res and activities o) li)e* #roteins are !iological polymers constr&cted )rom amino acid monomers* +here are seven ma/or classes o) proteins: :* Str&ct&ral proteins 8 incl&de hair, sil- o) spiders, )i!ers o) ligamentsEtendons, etc* 2* Contractile proteins D the type involved in m&scle movement "* Storage proteins D oval!&min 3egg ,hites4 )or developing em!ryos $* De)ensive proteins D anti!odies to )ight in)ection 7* +ransport proteins D hemoglo!in that transports oxygen in !lood stream F* Signal proteins D hormones that serve as messengers )rom one cell to another G* Enzyme proteins 8 serve as chemical catalysts in reg&lating almost all chemical activity 3reactions4 in the !ody -ore information about en$ymes Enzymes are large proteins that act as catalysts* .hat is a catalyst/ Catalysts either jump start or speed up the rate of a chemical reaction. Catalysts are recycled, they are not &sed &p in the reaction* Catalysts lo,er the amo&nt o) energy needed to t&rn reactants into prod&cts* 5emem!er: 5et&rn to 9nit : +a!le o) Contents
#rod&cts
A speci)ic enzyme catalyzes each cell&lar chemical reaction li-e each loc- )its only one -ey* Each enzyme has a part called the active site ,here the speci)ic s&!strate !inds* +he reactant is called the s&!strate* o 6n the hydrolysis o) s&crose, s&crose is the s&!strate 3reactant4* o S&crase is the name o) the enzyme that catalyzes this reaction* o +he enzyme allo,s hydrolysis 3adding a ,ater molec&le4 to happen* o +he molec&le o) s&crose is then !ro-en apart into a molec&le o) gl&cose and a molec&le o) )r&ctose* o +he enzyme s&crase goes on to )ind other s&crose molec&les and repeat the process* At the end o) the reaction, the s&!strate changes into the prod&ct and the enzyme is released* .hat are proteins made of/ )roteins are made from just 21 *inds of amino acids. All amino acids have the same !asic str&ct&re* +he 5 gro&p is ,hat varies in the 21 di))erent -inds o) amino acids* 0o% are proteins made/ )roteins are made by the process of dehydration synthesis.
2ucleic 'cids 0&cleic acids are in)ormation8rich polymers o) n&cleotides 0&cleic acids are the !l&eprints )or proteins +here are t,o types o) n&cleic acids: D0A D deoxyri!on&cleic acid 50A D ri!on&cleic acid Cenetic material consists o) D0A, and ,ithin the D0A are gene* Cenes are speci)ic stretches o) the molec&le that program the amino acid se2&ence o) proteins* .onomers that ma-e &p n&cleic acids are called n&cleotides* 50A is a single polyn&cleotide strand and D0A is a do&!le polyn&cleotide strand that t,ists to )orm a do&!le helix. 5et&rn to 9nit : +a!le o) Contents
)ictures in 3nstructional Bac*ground 3nformation: Camp!ell, 0eil* 32HHH4 Biology: Concepts and Connections* San Francisco, Addison ;esley ongman, 6nc*
Instructional Resources"
+his ,e!site has many hands on !iology activities* http:EEserendip*!rynma,r*ed&EsciIed&E,aldron +his ,e!site has lin-s to !iology la!s and lect&res* ,,,*explore!iology*com
5afety Concerns: St&dents sho&ld at least ,ear gloves ,hile per)orming tests )or car!ohydrate and proteins1 goggles are also recommended* >o& may also ,ant to -eep the 'i&ret reagent and iodine sol&tion at yo&r des- and have st&dents come to pic- it &p ,hen they need it* #eal&.orld Connections: St&dents relate st&dy o) macromolec&les to the n&trients in the )ood they eat* (eacher 2otes: +his activity rein)orces st&dent &nderstanding o) di))erent types o) organic compo&nds and several aspects o) scienti)ic method* 'e)ore yo& !egin this activity, yo&r st&dents sho&ld !e )amiliar ,ith the !asic chemical str&ct&res and general properties o) car!ohydrates, proteins and lipids* Help)&l teacher notes can !e )o&nd online at http:EEserendip*!rynma,r*ed&EexchangeE,aldronEorganic >o& ,ill need to ma-e evidence samples )or Day 2* ;e s&ggest that yo& ma-e )o&r di))erent pairs o) dry and li2&id evidence samples so di))erent st&dent gro&ps ,ill get di))erent res<s* .or*er in brea* room Jose Ashley 'r&ce Liara +unch75nac* 'ean !&rrito ,ith cheese Fat8)ree yog&rt +oast ,ith !&tter and /elly #retzels 5olid E!idence A ' C D 8lucose 8 ? ? 8 5tarch )rotein ? 8 ? ? ? ? 8 8 +i9uid E!idence A ' A ' +ipid ? 3oil4 8 3,ater4 ? 3oil4 8 3,ater4
)rocedure7Description of +esson: 6n the )irst class period, st&dents learn ho, to &se chemical indicators to test )or di))erent types o) organic compo&nds, and in the second class period each st&dent gro&p ,ill test one or t,o types o) )ood or a sample o) evidence to )ig&re o&t ,ho too- JerellMs i#od* >o& may ,ant to assign each type o) )ood or evidence to t,o st&dent gro&ps in order to assess relia!ility o) res<s* 5ource: Dr* Jenni)er Doherty, Dr* 6ngrid ;aldron and Dr* ori Spindler, Department o) 'iology,9niversity o) #ennsylvania, copyright 2HHN:, serendip*com, Adapted )rom A6dentity o) (rganic
Compo&ndsB )rom 'iology a!oratory .an&al A )rom #rentice8Hall1 Also inspired !y ACrime Scene ActivityB !y Lathy #aris, 'ethel High School
.ho too* :erell6s i)od/ && An organic compo&nd mystery Jerell is a :Hth grade st&dent at City High School ,ho ,or-s at .cDonald=s on the ,ee-ends* ;hile on !rea-, Jerell ,as st&dying )or his !iology test and listening to his ne, i#od* +here ,ere )o&r other ,or-ers ta-ing a !rea- at the same time, each having something di))erent )or l&nch* Jerell=s girl)riend stopped !y near the end o) his !rea-, and he r&shed o&t to see her and )orgot his i#od and !iology !oo- in the !rea- room* ;hen he realized he had )orgotten it, he h&rried !ac- and )o&nd only his !iology !oo- and some )ood cr&m!s* His i#od ,as goneO First, Jerell )rea-ed o&t, !&t he calmed do,n ,hen he realized he co&ld &se his -no,ledge o) organic compo&nds to )ig&re o&t ,hich o) his co,or-ers le)t the cr&m!s on his text!oo- and ,ho too- his i#od*
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.hat are organic compounds/ Almost all o) the )ood ,e eat comes )rom plants and animals* #lants and animals contain mainly ,ater and organic compo&nds, ,hich are molec&les made !y living organisms s&ch as plants or animals* +he ta!le !elo, lists the most common types o) organic compo&nds )o&nd in living organisms* For each type o) organic compo&nd, give one or t,o examples and descri!e one characteristic, e*g* ,hether it is greasy, ,hether it contains genetic material, ,hether there is lots o) this type o) organic compo&nd in meat or lots in pretzels and potatoes* DA+A +A' E :: (ype of ;rganic E amples of <ood (hat 0as Characteristic of <ood (hat Compound +ots of (his (ype of ;rganic 0as +ots of (his (ype of Compound ;rganic Compound Car!ohydrates ipids 0&cleic acids #roteins +oday yo& ,ill !e testing the s&!stances listed in the )ollo,ing ta!le* #redict ,hether each s&!stance is an organic compo&nd and i) so, ,hat type* DA+A +A' E 2: 5ubstance Do you thin* this substance is a carbohydrate, lipid, protein, or none of these/ %egeta!le oil Cl&cose Starch )rom corn or potatoes #o,dered egg ,hites ;ater .hat are indicators/ An indicator is a s&!stance that changes color in the presence o) a partic&lar type o) molec&le* +oday yo& ,ill learn ho, to &se several indicators to test )or the presence o) car!ohydrates and proteins* >o& ,ill also &se a di))erent type o) test )or lipids* +omorro,, yo& ,ill &se these tests to analyze several types o) )ood and the evidence le)t at the scene o) the crime to )ind o&t ,ho le)t the cr&m!s on Jerell=s text!oo-* (esting for lipids :* 6) a )ood that contains lipids is p&t on !ro,n paper, it ,ill leave a spot that lets light thro&gh* +o test )or lipids, divide a piece o) a !ro,n paper !ag into 7 sections* a!el the sections Pvegeta!le oilP, Pgl&coseP, PstarchP, Pegg ,hitesP, and A,aterB* 2* 6n each section, r&! a small amo&nt o) the s&!stance onto the !ro,n paper* 5et&rn to 9nit : +a!le o) Contents
;ith a paper to,el, r&! o)) any excess that may stic- to the paper* Set the paper aside &ntil the spots appear dryQa!o&t :H to :7 min&tes* "* ;hich section do yo& expect to test positive )or lipids< $* ;hich sections do yo& expect to test negative )or lipids< 7* Contin&e on ,ith the rest o) the tests* A)ter all the sections o) the !ro,n paper are dry, hold it &p to a !right light or ,indo,* >o& ,ill notice that at least one sample has le)t a spot that lets light thro&gh on the !ro,n paper* +he spot indicates the presence o) lipids* F* Complete the last col&mn o) data ta!le "* #&t a pl&s )or any samples ,hich tested positive )or lipids and a min&s )or the samples ,hich tested negative* DA+A +A' E ": Carbohydrate (ests 5ample +est strip color Cl&cose present 6odine test color Starch present )rotein (est 'i&ret test color #rotein present +ipid (est ipid present
%egeta!le oil Cl&cose Starch )rom corn or potatoes #o,dered egg ,hites ;ater (esting for Carbohydrates :* >o& m&st %ear glo!es to protect yourself* 2* >o& ,ill &se indicators to test )or t,o common types o) car!ohydrates: gl&cose 3a speci)ic type o) s&gar4 and starch* (!tain 7 containers and &se mas-ing tape to ma-e la!els )or each container* a!el the containers Pvegeta!le oilP, Pgl&coseP, PstarchP, Pegg ,hitesP, and A,aterB* "* For each container, add a small amo&nt o) the s&!stance indicated on the mas-ing8tape la!el* 0o, add a!o&t 2 ml o) ,ater to each container* Stir the contents o) each container to mix the sample and ,ater* 5et&rn to 9nit : +a!le o) Contents
+o test )or gl&cose yo& ,ill &se a test strip ,ith an indicator pad that changes color in the presence o) gl&cose* #repare a piece o) paper ,ith the name o) each s&!stance and a place to p&t the gl&cose test strip &sed to test that s&!stance* Dip one test strip into each sample )or :82 seconds* 5emove the strip, p&t it in the appropriate spot on yo&r la!eled paper, and ,ait " min&tes*
$* ;hich s&!stance do yo& expect to test positive )or gl&cose< 7* ;hich s&!stances do yo& expect to test negative )or gl&cose< F* A)ter " min&tes, record the color )or each gl&cose test strip in the data ta!le "* #&t a pl&s next to those samples testing positive )or gl&cose and a min&s )or those testing negative* G* +o test )or starch yo& ,ill &se iodine as an indicator* 6n the presence o) starch, iodine ,ill change color )rom yello,8!ro,n to !l&e8!lac-* Add 7 drops o) iodine sol&tion to each container* Stir the contents o) each container* C'=(3;2: Be careful when handling iodine; it can stain hands and clothing. R* 6n data ta!le ", record the color o) the iodine sol&tions* #&t a pl&s next to those samples testing positive )or starch and a min&s )or those testing negative* (esting for )roteins :* a!el )ive clean containers Pvegeta!le oilP, Pgl&coseP, PstarchP, Pegg ,hitesP, and A,aterB* Add a small amo&nt o) the s&!stance indicated on the la!el to each container* Add a!o&t 2 ml o) ,ater to each container* Stir the contents o) each container to mix the )ood and ,ater* 2* +o test )or protein yo& ,ill &se 'i&ret reagent as an indicator* 'i&ret reagent t&rns )rom !l&e to p&rple in the presence o) protein* Add 2H drops o) !i&ret reagent to each container* Stir the contents o) each container* C'=(3;2: Biuret reagent contains sodium hydroxide, a strong base. Be very careful not to splash or spill any. If you splash any reagent on yourself, wash it off immediately with water. Call your teacher for assistance. "* 5ecord the color o) each 'i&ret sol&tion in data ta!le "* #&t a pl&s next to those samples testing positive )or protein and a min&s )or those testing negative* $* 5inse all ten containers thoro&ghly* 4uestions :* Compare yo&r predictions )rom data ta!le 2 ,ith yo&r test res<s in data ta!le "* ;ere there any di))erences !et,een yo&r test res<s and yo&r predictions )or ,hat type o) organic compo&nd each test s&!stance is<
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6) yo& )o&nd any di))erences !et,een yo&r predictions and yo&r res<s, ,hat do yo& thin- is the reason )or these di))erences< >o& may ,ant to chec- ,ith yo&r teacher, yo&r text!oo-, or the n&trition in)ormation in the la!el on each )ood pac-age to help yo& interpret yo&r res<s* Did yo&r test )or gl&cose indicate there ,as gl&cose in the starch sample< Does that mean that there is no gl&cose in starch< 3Hint: Chec- yo&r text!oo- or other relia!le so&rce i) yo& do not already -no, the chemical str&ct&re o) starch*4 +his res< sho,s that the gl&cose indicator is 2&ite speci)ic* 6t reacts ,ith gl&cose dissolved in ,ater, !&t it does not react ,ith gl&cose molec&les that are com!ined into a large organic compo&nd li-e starch* ,- Suppose t$at for t$e container containing ,ater yo& )o&nd a positive test )or one o) the organic compo&nds* Ho, ,o&ld yo& interpret this res<< (esting Different (ypes of <ood and (esting the E!idence +oday yo& ,ill per)orm all )o&r organic compo&nd tests on one or t,o o) the types o) )ood listed !elo, or on the evidence that Jerell )o&nd at the crime scene 3yo&r teacher ,ill assign yo& samples to test4* 'egin !y predicting ,hich types o) compo&nds yo& expect to )ind in each type o) )ood yo& ,ill !e testing* DA+A +A' E $ <ood Do you e pect this food to contain 8lucose/ 5tarch/ )rotein/ +ipid/ #retzel '&tter Jelly Fat8)ree yog&rt 'eans 5ecord yo&r positive and negative test res<s &sing pl&s and min&s signs in the data ta!le !elo,* +he evidence that Jerell )o&nd has !een separated into a li2&id and a solid in t,o separate !ottles* A)ter yo& per)orm the tests, yo&r teacher ,ill collect yo&r data to share ,ith the rest o) the class* Complete the ta!le !elo, &sing data )rom yo&r classmates* DA+A +A' E 7 +ipid Carbohydrate (ests )rotein (est (est <ood +est strip Cl&cose 6odine Starch 'i&ret #rotein ipid color present test color present test color present present #retzel 3cr&m!le into the container4 '&tter Jelly Fat8)ree yog&rt 'eans 3mash into a paste4 5et&rn to 9nit : +a!le o) Contents
Dry part o) Jerell=s evidence i2&id part o) Jerell=s evidence .ho too* :erell6s i)od/ +he ,or-ers in the !rea- room are listed !elo, ,ith the type o) l&nch they ,ere eating ,hile Jerell ,as st&dying* As preparation )or interpreting the evidence, complete the chart !elo, to indicate ,hat -inds o) organic compo&nds are )o&nd in each type o) )ood and ,hat -inds o) organic compo&nds ,ere )o&nd in the com!ined li2&id ? dry evidence* .or*er in brea* +unch75nac* 8lucose 5tarch )rotein +ipid room Jose 'ean !&rrito ,ith cheese Ashley Fat8Free >og&rt 'r&ce +oast ,ith !&tter and /elly Liara #retzel +hie) Com!ined li2&id ? dry evidence Complete the )ollo,ing ta!le to s&mmarize the evidence and yo&r interpretation o) the evidence* Did he7she .or*er ta*e 0o% do you *no%/ in brea* :erell6s Describe the e!idence that supports your conclusion. room ipod/ Jose
Ashley
'r&ce
Liara
Analysis S&estions: :* ;ho too- Jerell=s i#od< Do yo& have any do&!ts a!o&t yo&r concl&sion< Explain*
2* (&r !odies are made &p o) the same types o) organic compo&nds as all other living organisms* Complete the )ollo,ing sentences !y )illing in each !lan- to indicate the )&nction o) each type o) molec&le in di))erent parts o) o&r !ody* (&r m&scles contain lots o) protein* +his protein ena!les the m&scles to IIIIIIIIIIIII* Cl&cose is carried !y o&r !lood to all the cells in o&r !ody* (&r cells &se the gl&cose )or IIIIIIIIIIIIIII* ipids are )o&nd in )at cells in o&r !odies* +he )at cells store )at molec&les to !e &sed )or IIIIIIIIIIIIII i) a person cannot get eno&gh )ood* (&r !odies do not ma-e starch, !&t ,e o)ten eat plant )oods ,hich contain starch ,hich ,e digest to IIIIIIIIIIIII, the !&ilding !loc- that is &sed to ma-e starch* D0A is a n&cleic acid that is )o&nd in every cell* D0A carries the IIIIIIIIIIII in)ormation* "* +o sho, yo&r &nderstanding o) organic compo&nds, identi)y the type o) organic compo&nd sho,n in each diagram and complete the )irst three col&mns o) the ta!le* $* .any large organic compo&nds are made o) m<iple repeats o) smaller !&ilding !loc- compo&nds* Starch, proteins, and n&cleic acids are examples o) this type o) organic compo&nd* Circle a !&ilding !loc- in the starch, protein, and n&cleic acid )ig&res, and ,rite the name o) the !&ilding !loc- in the )o&rth col&mn* .hich test is used to (ype of 2ame of Diagram of 5tructure of ;rganic <unctions detect this compound ;rganic building Compound or type of Compound bloc* compound/
Staple label here and highlight or underline high fructose corn syrup (HFCS)!
5et&rn to 9nit : +a!le o) Contents Staple label here and highlight or underline glucose/dextrose!
Staple label here that has ! high fructose corn syrup (HFCS)!
'ssessment 3deas: St&dent &nderstanding can !e assessed thro&gh class disc&ssion at the concl&sion o) the activity as ,ell as completion o) the assignment and article extension* Also, yo& can )ollo, &p this activity !y having st&dents cond&ct research on the prosEcons o) high )r&ctose corn syr&p and cond&ct a de!ate in class* +he de!ate co&ld !e done as a Tsilent de!ate*= +his is a ,riting strategy ,here each st&dent m&st pic- a side* +he )irst st&dent ,rites do,n a statement to s&pport their position, passes the paper to the second st&dent ,ho de!ates that statement ,ith their o,n arg&ment* +his contin&es )or a set amo&nt o) time 3UG min&tes4* (pposing vie,points can !e )o&nd at the )ollo,ing lin-s: .c a&ghlin, isa* A6s High8Fr&ctose Corn Syr&p 5eally Cood )or >o&<B +ime*com* Septem!er :G, 2HHR Verats-y, Latherine* AHigh8)r&ctose corn syr&p seems to !e a common ingredient in many )oods* ;hat are the concerns a!o&t high8)r&ctose corn syr&p<B .ayoclinic*com #rinceton 9niversity* PHigh8Fr&ctose Corn Syr&p #rompts Considera!ly .ore ;eight Cain, 5esearchers Find*P ScienceDaily 22 .arch 2H:H* 27 J&ne 2H:H Whttp:EE,,,*sciencedaily*com EreleasesE2H:HEH"E:HH"22:2:::7*htm 5et&rn to 9nit : +a!le o) Contents 5et&rn to 9nit : +a!le o) Contents
allO +hen the ,hole mess is !oiled )or ho&rs to extract the gelatinY and this part really ma-es a stin-O Finally, the gelatin layer is s-immed o)) the !oiling pot, and dried into a po,der* ;ith added s&gar, )lavorings, and arti)icial color, itMs ready to !ecome a /iggly dessertO And no, that yo& -no, ,hat Jell8(Ms made )rom, ,hy donMt yo& p&t some on the ta!le tonight< >o&r g&ests ,ill !e delighted ,hen yo& share yo&r ne, -no,ledge ,ith them in the middle o) a l&scio&s spoon)&l o) dessertO 'y the ,ay, this ,hole process o) extracting gelatin )rom !one ,as originally developed in :R$7 !y an engineer, #eter Cooper Q the man ,ho Cooper 9nion 3in 0>C4 is named a)ter* Sometime later 3:RN74, #earl '* ;ait, a co&gh syr&p man&)act&rer, !o&ght the patent )rom #eter Cooper and adapted CooperMs gelatin dessert into an entirely prepac-aged )orm, ,hich his ,i)e, .ay David ;ait, named PJell8(*P +he rest is history*** .ade )rom !oneY made )rom proteinY so it m&st !e to&gh st&))O So ,hy can=t yo& p&t )resh pineapple in it< et=s learn a !it a!o&t pineapple* +he pineapple plant 3"nanas co#osus4 is a monocot, or grass8li-e plant, that !elongs to the !romeliad )amily* 6t is tho&ght to have originated in 'razil* 6n the :N7Hs, pineapple !ecame the 9nited State=s second most important )r&it and Ha,aii led the ,orld in !oth 2&antity and 2&ality o) pineapples* Ho,ever, times have changed and no,, all canned pineapple comes )rom overseas, largely )rom the #hilippines* As ,ith some other tropical )r&its, the pineapple )r&it contains an enzyme that !rea-s do,n, or digests, protein* +his protease 3protein8digesting4 enzyme in pineapple is called bromelain, ,hich is extracted and sold in s&ch prod&cts as SchillingMs .eat +enderizer* #apaya, another tropical )r&it, also contains an enzyme, called papain, that digests protein* 6t can !e )o&nd in Accent .eat +enderizer* )rocedure: 6n this la!, yo& ,ill !e given an array o) materials and yo& ,ill !e as-ed to design yo&r o,n experiment to test the e))ect o) pineapple on gelatin* +he goal is to &nderstand ,hat is act&ally going on in the pineapple8gelatin mix at a chemical level as ,ell as &nderstanding ,hat a))ects the )&nction o) enzymes* Design a controlled experiment that sho,s the e))ect o) ra, pineapple on gelatin* .a-e s&re yo&r experiment description incl&des the )ollo,ing: A hypothesis A detailed experimental design ,hich ,ill incl&de: +he e))ect o) )resh pineapple on gelatin* +he e))ect o) )rozen pineapple on gelatin* +he e))ect o) canned pineapple on gelatin* +he e))ect o) )reshly coo-ed pineapple on gelatin* A data ta!le >o& ,ill !e a!le to per)orm yo&r experiment once yo& receive approval o) yo&r experimental design )rom yo&r teacher*
E perimental Design 8uide +eacher ApprovalIIIIIIIII +itle: Hypothesis: 6ndependent %aria!le: .eas&rement o) 6ndependent %aria!le: 0&m!er o) +rials: Dependent %aria!le: .eas&rement o) Dependent %aria!le: Control: (ther Controlled Factors 3At east 74: 4uestions: :* Clearly descri!e the res<s o) yo&r experiment* 6n ,hich test t&!es did the gelatin /ell, ,hich did not* 2* Clearly explain the res<s o) yo&r experiment* ;hy did some test t&!es o) gelatin /ell, ,hy did others not* 'e speci)icO "* ;hat is the enzyme in yo&r experiment< $* ;hat is the s&!strate in yo&r experiment< 7* ;hat is 3are4 the prod&ct3s4 in yo&r experiment< F* ;hat type o) organic molec&le is gelatin< G* ;hat type o) organic molec&le is !romelain< R* ;rite a A,ord e2&ationB to descri!e the chemical reaction that occ&rs ,hen pineapple is mixed ,ith the gelatin* N* 6s the reaction o) !romelain and gelatin dehydration synthesis or hydrolysis< Explain* :H* ;hy ,ere the res<s o) the )reshly coo-ed pineapple di))erent than the res<s o) the )resh, ra, pineapple< 'e speci)icO ::* ;hat is meat tenderizer and ,hat does it do< 'ssessment 3deas: (n the accompanying sheet o) paper, design an experiment to test at ,hat speci)ic temperature the pineapple enzyme denat&res* +itle: Hypothesis: 6ndependent %aria!le: .eas&rement o) 6ndependent %aria!le: 0&m!er o) +rials: Dependent %aria!le: .eas&rement o) Dependent %aria!le: Control: (ther Controlled Factors 3At east 74: 5et&rn to 9nit : +a!le o) Contents
5et&rn to 9nit : +a!le o) Contents A* Ho, does this relate to the n&m!er o) car!on, hydrogen, and oxygen atoms in the chemical )orm&la )or gl&cose< IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII 'cti!ity ': Cet the Cizmo ready: Build a glucose 'e s&re the C5EA+E C 9C(SE ta! is still selected* molecule 3ntroduction: Each element tends to )orm a certain n&m!er o) chemical !onds* +his val&e is the !alence o) the element* For example, a car!on atom has a valence o) )o&r* 8oal: Construct a molecule of glucose. :* 6denti)y: +he str&ct&re o) a ,ater molec&le 3H2(4 can !e ,ritten as H8(8H, ,hich each dash representing a chemical !ond* Co&nt the n&m!er o) !onds the oxygen and hydrogen atoms )orm in a ,ater molec&le* A* ;hat is the valence o) oxygen< IIIIIII IIIIIII '* ;hat is the valence o) hydrogen<
2* '&ild a model: 9se the car!on, oxygen, and hydrogen atoms )rom the 'toms bo to !&ild a gl&cose molec&le on the empty hexagon in the !&ilding region* 9se the chemical str&ct&re in the lo,er right as a g&ide, and pay attention to the valence o) each atom as yo& !&ild* (nce yo& thin- yo& have correctly constr&cted the gl&cose molec&le, clic- Chec** 6) necessary, contin&e to modi)y yo&r molec&le &ntil it is correct* "* .a-e a diagram: Congrat&lations, yo& have completed a molec&le o) gl&coseO Clicthe C(#> SC5EE0 !&tton to ta-e a snapshot o) yo&r completed molec&le* #aste the image into a !lan- doc&ment and la!el the image ACl&cose*B $* Explain: Ho, did the valence o) each element help yo& determine the str&ct&re o) the gl&cose molec&le< 7* .a-e connections: Car!on )orms the !ac-!one o) every ma/or type o) !iological molec&le, incl&ding car!ohydrates, )ats, proteins, and n&cleic acids* Ho, does car!on=s high valence relate to its a!ility to )orm these large and complex !iomolec&les< Cet the Cizmo ready: Select the DEH>D5A+6(0 ta!*
5et&rn to 9nit : +a!le o) Contents 4uestion: .hat occurs %hen t%o glucose molecules bond/ :* 6n)er: Cl&cose is an example o) a monosaccharide, the simplest type o) car!ohydrate* A disaccharide is made )rom !onding t,o monosaccharides together* A* ;hat do yo& thin- the pre)ixes #ono% and di% mean< &ono%: IIIIIIIIII $i%: IIIIIIIIII 2* #redict: +&rn on 5ho% description* Drag !oth gl&cose molec&les into the !&ilding region* (!serve the highlighted region* ;hat do yo& thin- ,ill happen to the atoms in this region ,hen the gl&cose molec&les !ond< "* 5&n Cizmo: Clic- Continue and ,atch the animation* A*;hat happened< '*;hat ,as removed )rom the gl&cose molec&les ,hen they !onded to )orm maltose< $* 6n)er: 'ased on ,hat yo& have seen, create a !alanced e2&ation )or the dehydration synthesis reaction* 35ecall that the )orm&la )or gl&cose is CFH:2(F*4 >o& ,ill have to determine the )orm&la o) maltose yo&rsel)* 7*+&rn on 5ho% current formula7e9uation to chec- yo&r ans,er* F*S&mmarize: 9se ,hat yo& have o!served to explain ,hat occ&rs d&ring a dehydration synthesis reaction* G*Apply: A trisaccharide is a car!ohydrate made o) three monosaccharides* ;hat do yo& thin- ,o&ld !e the chemical )orm&la o) a trisaccharide made o) three !onded gl&cose molec&les< Cet the Cizmo ready:
Select the Hydrolysis ta!* +&rn on 5ho% description and 5ho% current 0ydrolysis formula7e9uation* 3ntroduction: Car!ohydrates made &p o) three or more !onded monosaccharides are -no,n as polysaccharides* 6n a reaction -no,n as hydrolysis, yo&r !ody !rea-s do,n polysaccharides into individ&al monosaccharides that can !e &sed !y yo&r cells )or energy* :* #redict: Examine the polysaccharide in the !&ilding region and its chemical )orm&la* A* Ho, many monosaccharides can )orm i) this polysaccharide !rea-s &p<
'cti!ity C:
'* 5ecall the )orm&la o) gl&cose is CFH:2(F* Ho, many car!on, oxygen, and hydrogen atoms ,ill yo& need )or three gl&cose molec&les< 5et&rn to 9nit : +a!le o) Contents C* ;hat m&st !e added to the polysaccharide in the Cizmo to get three gl&cose molec&les< 2* (!serve: +&rn o)) 5ho% current formula7e9uation* Drag a ,ater molec&le into the !&ilding region* Clic- Continue* ;hat happened<
"* 6n)er: Create a !alanced e2&ation )or the hydrolysis reaction that /&st occ&rred* +&rn on 5ho% current formula7e9uation to chec- yo&r ans,er* $* 7* (!serve: +&rn o)) 5ho% current formula7e9uation* Drag the second ,ater molec&le into the !&ilding region* Clic- Continue* ;hat happened< S&mmarize: 0o, create a !alanced e2&ation that sho,s the entire hydrolysis reaction* 36n other ,ords, the e2&ation sho&ld sho, ho, the polysaccharide !ro-e &p into three separate gl&cose molec&les*4
+&rn on 5ho% current formula7e9uation to chec- yo&r ans,er* F* Compare: Ho, do hydrolysis reactions compare to dehydration synthesis reactions< G* Apply: Amylose is a polysaccharide made )rom the synthesis o) )o&r gl&cose molec&les* A* Ho, many ,ater molec&les are prod&ced ,hen amylose )orms< '* ;hat do yo& thin- is the chemical )orm&la )or amylose< C* Ho, many ,ater molec&les ,o&ld !e needed to !rea- amylose do,n into )o&r gl&cose molec&les< R* Extend yo&r thin-ing: Hydrolysis o) the car!ohydrates yo& eat !egins in yo&r mo&th as yo& che,* Ho, do yo& thin- this process might !e a))ected i) a person=s salivary glands ,ere &na!le to prod&ce saliva, ,hich is mostly composed o) ,ater< 'ssessment 3deas: 8um Drop Chemistry Have st&dents &se their diagrams o) a gl&cose molec&le )rom activity A o) the St&dent Exploration sheet to !&ild a model o) gl&cose &sing toothpic-s and g&m drops 3or mini marshmallo,s4* .a-e s&re all st&dents &se the same color o) g&m drops to represent each type o) atom* ;rite the color -ey on the !oard as a reminder )or st&dents* A)ter each st&dent !&ilds their g&m drop model, have st&dents )orm pairs* +he st&dents sho&ld model ho, t,o gl&cose molec&les can !ond to )orm a molec&le o) maltose and a 5et&rn to 9nit : +a!le o) Contents
molec&le o) ,ater* 0ext, have st&dents model hydrolysis !y reversing the reaction* +his can also !e done ,ith molec&lar -its* 5et&rn to 9nit : +a!le o) Contents